Prenol

Manufactured by Merck Group

Sourced in Germany

Prenol is a laboratory equipment product manufactured by Merck Group. It is a high-quality device designed for precise and reliable laboratory work. Prenol's core function is to provide a controlled environment for various scientific experiments and analyses.

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Lab products found in correlation

5 protocols using prenol

Characterization of Aroma Compounds

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N-Alkanes (C7–C30) used to calculate the retention indices (RI values) were obtained from Sigma-Aldrich (Milwaukee, WI, USA). All standards (β-pinene, (−)-β-elemene, prenol, 2-methyl-2-butenal, heptanal, octanal, nonanal, 6-methyl-5-hepten-2-one, caryophyllene, and acetic acid) used for qualitative and quantitative analysis of the aroma compounds were purchased from Sigma-Aldrich. Nitrogen (99.9992% purity) and helium (99.999% purity) were purchased from Beijing Haipubeifen Gas Industry Co. Ltd. (Beijing, China). Liquid nitrogen (99.99% purity) was purchased from Beijing Xianheyu Gas Industry Co. Ltd. (Beijing, China).

Hu D., Guo J., Li T., Zhao M., Zou T., Song H, & Alim A. (2019). Comparison and Identification of the Aroma-Active Compounds in the Root of Angelica dahurica. Molecules, 24(23), 4352.

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Synthesis of Vanillyl Ferulate Ester

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VFA was prepared in 52% overall yield after three steps starting from FA. The procedure was modified from Mastihubova and Mastihuba (2013 (link)), offering >95% purity after column chromatography on silica gel based on ¹H NMR and LC-MS. Methyl ferulate (MFA) was purchased from Alfa Aesar (Karlsruhe, Germany), while FA, prenol (99%), n-hexane (<0.02% water), t-butanol (anhydrous, ≥99.5%), MOPS solution 1 M, and other materials were purchased from Sigma-Aldrich (Saint Louis, USA).

Antonopoulou I., Leonov L., Jütten P., Cerullo G., Faraco V., Papadopoulou A., Kletsas D., Ralli M., Rova U, & Christakopoulos P. (2017). Optimized synthesis of novel prenyl ferulate performed by feruloyl esterases from Myceliophthora thermophila in microemulsions. Applied Microbiology and Biotechnology, 101(8), 3213-3226.

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Transesterification of Organic Alcohols

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All organic alcohols (geraniol, prenol) and methyl acetate used for the transesterification had purities above 98% and were purchased from Sigma-Aldrich (Schnelldorf, Germany). The desiccant (Molecular Sieve UOP Type 5 Å, Sigma-Aldrich, Schnelldorf, Germany), used to remove methanol, consisted of circular beads with a pore-width of 5 Å. The desiccants were dried for 20 min at 150 °C prior to use.

Van Hecke W., Debergh P., Khan M.N, & Van Dael M. (2021). Rational Design Method Based on Techno-Economic Principles for Integration of Organic/Organic Pervaporation with Lipase Catalyzed Transesterification. Membranes, 11(6), 407.

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Extraction and Purification of Ferulate Compounds

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VFA (vinyl ferulate) was provided by Taros Chemicals GmbH & Co. KG (Dortmund, Germany). Methyl ferulate (MFA) was purchased from Alfa Aesar (Karlsruhe, Germany), while FA, prenol (99%), n-hexane (<0.02% water), t-butanol (anhydrous, ≥99.5%), MOPS solution 1 M, and other materials were purchased from Sigma-Aldrich (Saint Louis, MO, USA).

Zerva A., Antonopoulou I., Enman J., Iancu L., Jütten P., Rova U, & Christakopoulos P. (2018). Optimization of Transesterification Reactions with CLEA-Immobilized Feruloyl Esterases from Thermothelomyces thermophila and Talaromyces wortmannii. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(9), 2403.

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Feruloyl Esterases Characterization from T. wortmannii

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The feruloyl esterases Fae125, Fae7262 and Fae68 from T. wortmannii, over-expressed individually in low background C1-expression strains, were provided by DuPont Industrial Biosciences (Wageningen, the Netherlands) in the form of lyophilized powder. Methyl ferulate (MFA) was purchased from Alfa Aesar (Karlsruhe, Germany). n-Hexane (<0.02% water), t-butanol (anhydrous, ≥ 99.5%), prenol (99%), L-arabinose (≥99%), FA, MOPS solution 1 M and other chemicals were purchased from Sigma-Aldrich (Saint Louis, USA). VFA was provided by Taros Chemicals GmbH & Co. KG (Dortmund, Germany).

Antonopoulou I., Iancu L., Jütten P., Piechot A., Rova U, & Christakopoulos P. (2018). Optimized Enzymatic Synthesis of Feruloyl Derivatives Catalyzed by Three Novel Feruloyl Esterases from Talaromyces wortmannii in Detergentless Microemulsions. Computational and Structural Biotechnology Journal, 16, 361-369.

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