Piceatannol

For TCR signaling inhibition, the Syk-Zap70 kinase inhibitor piceatannol (Sigma Cat# P0453) was used. Mice were treated with daily i.p. injections of 500 µg resuspended in sterile corn oil on days −1 and 0 prior to infection. For mTOR inhibition, mice were treated with daily i.p. injections of rapamycin (Sigma Cat# R0395). rapamycin was dissolved in ethanol to a concentration of 2mM and further diluted in sterile PBS for i.p. injection at a dose of 1.5mg/kg per day in 100 µL PBS on day −1, 0 and +1 of Salmonella infection. For glycolysis inhibition, animals were treated with i.p. injections of 400 mg/kg of 2-DG (Sigma Cat# D6134) on day −1, day 0 and day +1 of Salmonella infection. For glycolysis pathway activation, animals were injected with 250 mg/kg Metformin (USP Cat#136309) in 100 µL PBS daily for 3 days and cells analyzed 2–3 hours after last injection. For tamoxifen treatment in inducible Cre-recombinase systems, mice were i.p. injected with 0.8 mg of tamoxifen resuspended in corn oil at time points as specified in figure legends. For LPS exposure experiments, GF or ABX-treated mice were exposed to 100 µg/ml of lipopolysaccharide (LPS) from Salmonella enterica serotype Typhimurium (Sigma Cat# L6511) in drinking water, filtered (0.22µm), for 7 days prior to analysis.