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Transcriptor reverse transcription kit

Manufactured by Roche
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The Transcriptor Reverse Transcription Kit is a laboratory product designed for the synthesis of complementary DNA (cDNA) from RNA templates. The kit contains the necessary reagents and enzymes required to perform reverse transcription, a key step in the process of converting RNA into DNA for further analysis or applications.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (3 mentions) Rneasy micro kit, by Qiagen (3 mentions) Lc480 light cycler, by Roche (1 mentions) 7 8 dihydroxyflavone 7 8 dhf, by Bio-Techne (1 mentions) Brain derived neurotrophic factor (bdnf), by R&D Systems (1 mentions) Trkb fc chimera, by R&D Systems (1 mentions)

4 protocols using transcriptor reverse transcription kit

1

Quantitative RT-PCR Analysis of Mouse Lung

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Mouse lung tissue and frozen sections were prepared under RNase-free conditions. Total RNA was extracted from lung tissue using the RNeasy Mini Kit (Qiagen, Valencia, CA) while total RNA was isolated from LCM caps using the RNeasy micro kit (Qiagen). Using the transcriptor reverse transcription kit (Roche, Indianapolis, IN) complementary (cDNA) was synthesized. Real-time PCR was performed using LC480 LightCycler (ABI, Carlsbad, CA). Each cDNA sample was plated in triplicate and data in each category (Saline/RA, Saline/O2, LPS/RA, and LPS/O2) were pooled for statistical analysis. Message RNA (mRNA) expression was calculated by the normalization of cycle threshold [C(t)] values of target gene to reference gene (ribosomal protein S16). The relative fold change was calculated using the ΔΔCt method. Primers used for qRT-PCR are listed in Table 1.
Faksh A., Britt RD J.r., Vogel E.R., Kuipers I., Thompson M.A., Sieck G.C., Pabelick C.M., Martin R.J, & Prakash Y. (2015). Effects of Antenatal Lipopolysaccharide and Postnatal Hyperoxia on Airway Reactivity and Remodeling in a Neonatal Mouse Model. Pediatric research, 79(3), 391-400.
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2

Quantifying Gene Expression Changes in ASM Cells Exposed to Cigarette Smoke Extract

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ASM cells were treated with 1% CSE for 48 h and total RNA isolated using RNeasy mini kit (Qiagen, Valencia, CA). Complementary DNA (cDNA) was prepared using the Transcriptor reverse transcription kit (Roche, Indianapolis, IN), and was used as a template for RT-PCR optimized for the Roche LC480 Light Cycler, with S16 as internal control. The ΔΔC(t) method as above was used to determine changes in expression of mRNA of interest. Unexposed control was used as the calibrator for quantification. Primers used for RT-PCR are listed in Table 2.
Aravamudan B., Thompson M., Sieck G.C., Vassallo R., Pabelick C.M, & Prakash Y.S. (2016). Functional Effects of Cigarette Smoke-Induced Changes in Airway Smooth Muscle Mitochondrial Morphology. Journal of cellular physiology, 232(5), 1053-1068.
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3

Quantitative RT-PCR Analysis of Mouse Lung

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse lung tissue and frozen sections were prepared under RNase-free conditions. Total RNA was extracted from lung tissue using the RNeasy Mini Kit (Qiagen, Valencia, CA) while total RNA was isolated from LCM caps using the RNeasy micro kit (Qiagen). Using the transcriptor reverse transcription kit (Roche, Indianapolis, IN) complementary (cDNA) was synthesized. Real-time PCR was performed using LC480 LightCycler (ABI, Carlsbad, CA). Each cDNA sample was plated in triplicate and data in each category (Saline/RA, Saline/O2, LPS/RA, and LPS/O2) were pooled for statistical analysis. Message RNA (mRNA) expression was calculated by the normalization of cycle threshold [C(t)] values of target gene to reference gene (ribosomal protein S16). The relative fold change was calculated using the ΔΔCt method. Primers used for qRT-PCR are listed in Table 1.
Faksh A., Britt RD J.r., Vogel E.R., Kuipers I., Thompson M.A., Sieck G.C., Pabelick C.M., Martin R.J, & Prakash Y. (2015). Effects of Antenatal Lipopolysaccharide and Postnatal Hyperoxia on Airway Reactivity and Remodeling in a Neonatal Mouse Model. Pediatric research, 79(3), 391-400.
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4

Gene Expression Analysis of BDNF Signaling

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PAECs were treated, under either normoxia or hypoxia, with 1 nM BDNF (R&D Systems), 1μg/ml TrkB-Fc chimera (R&D Systems), 10μM HIF-1α inhibitor, or 1μM 7,8-Dihydroxyflavone (7,8-DHF; Tocris, Minneapolis, MN), a BDNF agonist. Total RNA was isolated from these cells, using the RNeasy micro kit (Qiagen, Valencia, CA). Complementary DNA (cDNA) was synthesized using Transcriptor reverse transcription kit (Roche, Indianapolis, IN), and was amplified using an LC480 LightCycler (ABI; Carlsbad, CA), and primers listed in Table 1. Real-Time PCR was performed in duplicates per cDNA template, and data for all cDNAs in a category (normoxia, or hypoxia; untreated controls or agonist/inhibitor-treated) were pooled for statistical analysis. All PCR reactions went through 60 amplification cycles. The ratio of fold change in expression of the mRNA of interest for each sample was calculated by normalization of cycle threshold [C(t)] values of the target gene (e.g. BDNF, iNOS etc) to the reference gene (S16) using the comparative C(t) (ΔΔC(t)) method. Data are reported as the ΔC(t) and the average ratio of fold change in mRNA of interest corrected for reference gene. Normoxic, untreated controls were used as calibrator for quantification.
Helan M., Aravamudan B., Hartman W.R., Thompson M.A., Johnson B.D., Pabelick C.M, & Prakash Y.S. (2014). BDNF Secretion by Human Pulmonary Artery Endothelial Cells in Response to Hypoxia. Journal of molecular and cellular cardiology, 68, 89-97.
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