Ht1080

Mouse embryonic fibroblasts (MEFs) with homozygous deletion of the Lmna gene, which encodes lamins A/C, along with wild-type littermate controls, were generously provided by Dr. Colin Stewart.^{38 (link)} Wild-type MEFs were stably modified with lentiviral vectors to express mNeonGreen-Histone 2B,^{39 (link)} as described previously.⁴⁰ HT1080 cells were purchased from the DSMZ Braunschweig, Germany, and stably modified with lentiviral vectors to express the nuclear rupture reporter NLS-GFP, as described previously.⁴¹ Induced pluripotent stem cells (iPSC) and healthy human skin fibroblasts were generously provided by Elisa di Pasquale and Gianluigi Condorelli (Humanitas Clinical and Research Center, Italy).⁴² MDA-MB-231 cells were obtained from the American Type Culture Collection (ATCC). MEF, HT-1080, MDA-MB-231, and human fibroblast cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 % (v/v) fetal bovine serum and 1 % (v/v) penicillin/streptomycin. iPSCs were maintained on matrigel-coated dishes in mTeSR medium (Stem Cell Technologies), prepared according to manufacturer’s instruction. The dishes were prepared by diluting 50 μl matrigel (BD 354277) in 1 ml of mTeSR and incubating in 35 mm plastic petri dishes overnight at 4°C.

The breast adenocarcinoma cell line MDA-MB-231 (ATCC HTB-26) and the breast ductal carcinoma cell line BT-549 (ATCC HTB-122) were purchased from American Type Culture Collection (ATCC); the fibrosarcoma cell line HT1080 (ACC 315) was a gift from Peter Friedl and Katarina Wolf and originally purchased from the DSMZ Braunschweig, Germany; the hTERT-immortalized retinal epithelial cell line RPE-1 was a gift from Marcus Smolka; the SV40-immortalized human fibroblasts were purchased from the Coriell Institute (GM00637). MDA-MB-231, HT1080, RPE-1 and SV40-immortalized human fibroblast cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS, Seradigm VWR) and 1% (v/v) penicillin and streptomycin (PenStrep, ThermoFisher Scientific). BT-549 cells were grown in RPMI 1640 media supplemented with 10% FBS and 1% PenStrep. All cell lines were cultured under humidified conditions at 37°C and 5% CO₂ and verified using STR profiling services from ATCC.