Human DRGs were fixed in 4% paraformaldehyde overnight and then sections (12 μm) were cut on a cryostat. The sections were first blocked with 2% BSA for 1 h at room temperature, then incubated with peripherin (1:500, rabbit, Sigma) or a mixture of primary polyclonal TRPV1 antibody (1:400, rabbit, Neuromics, Edina, MN), and monoclonal NF200H antibody (1:1000, mouse, Sigma) overnight at 4°C. The sections were then incubated for 2 h at room temperature with cyanine 3 (Cy3)- and FITC-conjugated secondary antibodies (1:400; Jackson ImmunoResearch, West Grove, PA). The DRG sections were then stained with DAPI (1:1000, Sigma, for 5 min) and examined under a Nikon fluorescence microscope. Images were captured with a CCD Spot camera and analyzed with NIH Image J software or Adobe PhotoShop.
Nf200h antibody
Manufactured by Merck Group
The NF200H antibody is a laboratory research tool used to detect the presence of neurofilament heavy polypeptide (NF-H) protein in biological samples. NF-H is a structural component of the neuronal cytoskeleton and is commonly used as a marker for neurons and nerve fibers. The NF200H antibody can be utilized in various immunological techniques, such as Western blotting and immunohistochemistry, to identify and study NF-H expression in cells and tissues.
Automatically generated - may contain errors
2 protocols using nf200h antibody
1
Immunohistochemical Characterization of DRG Neurons
2
Immunohistochemical Visualization of Neuronal Markers
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Human DRGs were fixed in 4% paraformaldehyde overnight and then sections (12 μm) were cut on a cryostat. The sections were first blocked with 2% BSA for 1 h at room temperature, then incubated with peripherin (1:500, rabbit, Sigma) or a mixture of primary polyclonal TRPV1 antibody (1:400, rabbit, Neuromics, Edina, MN) and monoclonal NF200H antibody (1:1000, mouse, Sigma) overnight at 4 °C. The sections were then incubated for 2 h at room temperature with cyanine 3 (Cy3)- and FITC-conjugated secondary antibodies (1:400; Jackson ImmunoResearch, West Grove, PA). The DRG sections were then stained with DAPI (1:1000, Sigma, for 5 min) and examined under a Nikon fluorescence microscope. Images were captured with a CCD Spot camera and analyzed with NIH Image J software or Adobe PhotoShop.
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