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Phosphoserine10 histone h3

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Phosphoserine10-histone H3 is a lab equipment product that specifically detects the phosphorylation of serine 10 on histone H3. This modification is associated with chromosome condensation during cell division.

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Lab products found in correlation

Histone h3, by Abcam (2 mentions) Odyssey analyzer, by LI COR (2 mentions) β actin, by Santa Cruz Biotechnology (2 mentions) Aurora kinase b, by Merck Group (2 mentions) Molecular imager chemidoc xrs system, by Bio-Rad (1 mentions) Image lab software, by Bio-Rad (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Pierce protein g magnetic beads, by Thermo Fisher Scientific (1 mentions) β actin 1 19, by Santa Cruz Biotechnology (1 mentions) Ku70 n3h10, by Santa Cruz Biotechnology (1 mentions) Ha ha 7, by Merck Group (1 mentions) Ku80 c 20, by Santa Cruz Biotechnology (1 mentions) Clarity western ecl substrate, by Bio-Rad (1 mentions)

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Histone H3 (504 citations)

3 protocols using phosphoserine10 histone h3

1

Co-Immunoprecipitation and Western Blot Analysis

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Nuclear Extracts were prepared as described previously73 (link). For co-immunopreciptation experiments, extracts were adjusted to 0.15% NP-40 and 100 mM KCl, and incubated at 4 °C with either Ku70 antibody (N3H10, Santa Cruz, Santa Cruz, CA), or Ku80 antibody (C-20, Santa Cruz). Immunoprecipitates were isolated with Pierce Protein G magnetic beads (ThermoFisher Scientific, Rockford, IL). For Western blot analysis, extracts were resolved by SDS-PAGE, transferred onto a PVDF (Polyvinylidene fluoride) membrane and hybridized with the following antibodies: β-actin (I-19, Santa Cruz), Ku70 (N3H10, Santa Cruz), Ku80 (C-20, Santa Cruz), HA (HA-7, Sigma), p21 (C-19, Santa Cruz), phospho-serine 1981 ATM (Pierce, ThermoFisher Scientific), ATM (Pierce, ThermoFisher Scientific), phospho-serine 10 Histone H3 (Cell Signaling, Danvers, MA), Aurora B (H-75, Santa Cruz), FLAG (Sigma-Aldrich). The blots were developed using the Clarity Western ECL substrate (Bio-rad, Hercules, CA) and imaged on the Molecular Imager® ChemiDocTM XRS system (Bio-Rad). Quantifications were performed using Image Lab software (Bio-Rad).
Fell V.L., Walden E.A., Hoffer S.M., Rogers S.R., Aitken A.S., Salemi L.M, & Schild-Poulter C. (2016). Ku70 Serine 155 mediates Aurora B inhibition and activation of the DNA damage response. Scientific Reports, 6, 37194.
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2

Histone H3 Phosphorylation Assay

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HCT-116 cells were treated with 10 μM of DMSO, barasertib, GSK1070916, MLN8054, BRD-7880, or tozasertib. Cells lysates were probed on Western blot using antibodies (diluted 1:1000) to histone H3 (Abcam cat. 24834), phosphoserine10-histone H3 (Cell Signaling Technology cat no. 3377SS), aurora kinase B (Millipore cat. no. 04-1036), or beta-actin (Santa Cruz Biotechnology cat. no. sc-47778) and detected using a LI-COR Odyssey analyzer (LI-COR Biosciences, Lincoln, NE).
Yu C., Mannan A.M., Yvone G.M., Ross K.N., Zhang Y.L., Marton M.A., Taylor B.R., Crenshaw A., Gould J.Z., Tamayo P., Weir B.A., Tsherniak A., Wong B., Garraway L.A., Shamji A.F., Palmer M.A., Foley M.A., Winckler W., Schreiber S.L., Kung A.L, & Golub T.R. (2016). High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines. Nature biotechnology, 34(4), 419-423.
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3

Histone H3 Phosphorylation Assay

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HCT-116 cells were treated with 10 μM of DMSO, barasertib, GSK1070916, MLN8054, BRD-7880, or tozasertib. Cells lysates were probed on Western blot using antibodies (diluted 1:1000) to histone H3 (Abcam cat. 24834), phosphoserine10-histone H3 (Cell Signaling Technology cat no. 3377SS), aurora kinase B (Millipore cat. no. 04-1036), or beta-actin (Santa Cruz Biotechnology cat. no. sc-47778) and detected using a LI-COR Odyssey analyzer (LI-COR Biosciences, Lincoln, NE).
Yu C., Mannan A.M., Yvone G.M., Ross K.N., Zhang Y.L., Marton M.A., Taylor B.R., Crenshaw A., Gould J.Z., Tamayo P., Weir B.A., Tsherniak A., Wong B., Garraway L.A., Shamji A.F., Palmer M.A., Foley M.A., Winckler W., Schreiber S.L., Kung A.L, & Golub T.R. (2016). High-throughput identification of genotype-specific cancer vulnerabilities in mixtures of barcoded tumor cell lines. Nature biotechnology, 34(4), 419-423.
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