4 4 diisothiocyanatostilbene 2 2 disulfonic acid disodium salt hydrate

MAPK Family Antibody Sampler Kit (#9926) and Phospho-MAPK Family Antibody Sampler Kit (#9910) were purchased from Cell Signaling Technology (Beverly, MA). Anti-CEP-1 (cC-18) (sc-135460) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Paraquat (PQ), Cyclosporin A (CsA), Bongkrekic acid (BA) and 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate (DIDS) were from Sigma. N-acety-L-cysteine (NAC) was purchased from TCI (Shanghai, China). Total Antioxidant Capacity Assay Kit with the ABTS Method (S0119), Fluo-3 AM (S1056), Hoechst 33342 (C1022), Mitochondrial Membrane Potential Assay Kit with JC-1 (C2006), Enhanced BCA Protein Assay Kit (P0010S) and Superoxide dismutase (SOD, S0088) were obtained from Beyotime (Shanghai, China). Acridine orange (AO) was from Dingguo Changsheng Biotechnology (Beijing, China). H₂DCF-DA (D399), MitoSox (M36008), Mitotracker red (M7512), ATP determination kit (A22066) and SYTO 12 (S7574) were purchased from Molecular Probes (Eugene, Oregon, USA). PVDF membranes and ECL plus detection kit were obtained from Millipore (Bedford, MA, USA).

Ibipinabant (S-SLV 319) was ordered at Cayman Chemical (Ann Arbor, MI). A racemic mixture of 3-(4-chlorophenyl)-4-phenyl-4,5-dihydro-1H-pyrazole-1-carboxamide derivate 23 (CB23) was kindly provided by Abbott Products GmbH (Hannover, Germany). Etoposide, 4,4′-diisothiocyanatostilbene-2,2′-disulfonicacid disodium salt hydrate (DIDS), and atractyloside sodium salt were from Sigma Aldrich (Zwijndrecht, The Netherlands).

Tunicamycin, 4,4′-Diisothiocyanatostilbene-2,2′-disulfonic acid disodium salt hydrate (DIDS), 4-(2-butyl-6,7-dichlor-2-cyclopentyl- indan-1-on-5-yl)oxybutyric acid (DCPIB), 5-bromo-2-deoxyuridine (BrdU), 3-(4,5- dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), N-acetyl-l-cysteine (NAC) and antibody for β-actin were purchased from Sigma-Aldrich Corporation (St. Louis, MO, USA). sFRP was purchased from R&D Systems (Minneapolis, MN, USA). Antibody against GRP78 was from Bioworld Technology (St. Louis Park, MN, USA). Antibodies against CHOP and ATF4 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Phospho-eIF2α, total eIF2α, β-catenin and α-actinin were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody against Lamin B was from Boster (Wuhan, China). Dihydroethidium (DHE) was from Molecular Probes (Eugene, OR, USA). Terminal Deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) was from Roche Applied Science (Sandhofer Strasse, Mannheim, Deutschland).