Media and cell culture reagents

Manufactured by Sartorius

Sourced in Israel

Sartorius media and cell culture reagents are a range of products designed to support the growth and maintenance of cells in laboratory settings. These products provide the necessary nutrients, growth factors, and other essential components for optimal cell culture performance.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant tgf β1, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter dlr assay system, by Promega (1 mentions) Cy3 streptavidin, by Jackson ImmunoResearch (1 mentions) Sc 528, by Santa Cruz Biotechnology (1 mentions) Fatty acid free bovine serum albumin fraction 5, by Merck Group (1 mentions) Mouse anti β actin, by Merck Group (1 mentions) 125i tgf β1, by PerkinElmer (1 mentions) 4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (1 mentions) Penicillin streptomycin, by Sartorius (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Sartorius (1 mentions) L glutamine, by Sartorius (1 mentions)

Close spelling variants of this product

Cell culture reagents (5 citations) Cell culture media (4 citations)

2 protocols using media and cell culture reagents

TGF-β Signaling Pathway Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant TGF-β1 was obtained from PeproTech (Rocky Hill, NJ) and fatty acid–free bovine serum albumin (BSA; fraction V) from Sigma-Aldrich (St. Louis, MO). Media and cell culture reagents were from Biological Industries (Beit Haemek, Israel) or Invitrogen (Carlsbad, CA). Rabbit immunoglobulin G (IgG) against Smad3 (reactive with Smad3 and Smad2; sc-528) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA). G418 was purchased from Calbiochem (La Jolla, CA). Dual-luciferase reporter (DLR) assay system was from Promega (Fitchburg, WI). Affinity-purified biotinylated goat anti-rabbit (GαR) IgG and Cy3-streptavidin were obtained from Jackson ImmunoResearch Laboratories (West Grove, PA). Anti-myc tag (αmyc) 9E10 mouse ascites (Evan et al., 1985 (link)) and HA.11 rabbit antiserum to the HA tag (αHA) were from Covance Research Products (Denver, PA). IgG and monovalent Fab′ fragment αmyc were prepared from the 9E10 ascites as described (Henis et al., 1994 (link)). Alexa Fluor 488–GαR IgG and Alexa Fluor 546–goat anti-mouse (GαM) F(ab′)₂ were from Invitrogen-Molecular Probes (Eugene, OR); fluorescent F(ab′)₂ was converted into Fab′ as described (Gilboa et al., 1998 (link)). [¹²⁵I]TGFβ-1 was from PerkinElmer (Waltham, MA). Goat IgG against the extracellular domain of TβRIII was from R&D Systems (Minneapolis, MN). Mouse anti–β-actin was from Sigma-Aldrich.

Tazat K., Hector-Greene M., Blobe G.C, & Henis Y.I. (2015). TβRIII independently binds type I and type II TGF-β receptors to inhibit TGF-β signaling. Molecular Biology of the Cell, 26(19), 3535-3545.

+ Open protocol

+ Expand

Recombinant BMP2, BMP9, and ActA Cytokine Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant human BMP2 (cat. #120-02C), BMP9 (cat. #120-07), and ActA (cat. #120-14P) were obtained from PeproTech (Rocky Hill, NJ). Media and cell culture reagents (fetal calf serum, L-glutamine, penicillin-streptomycin (25 and 40 μg/ml, respectively) and Hanks’ balanced salt solution (HBSS) were from Biological Industries Israel (Beit Haemek, Israel). Fatty acid-free bovine serum albumin (BSA) (fraction V; cat. #10-775-835-001) were obtained from Roche Diagnostics (Manheim, Germany). Phosphate-buffered saline, protease inhibitor cocktail (cat. #P8340), Na₃VO₄, LDN212854 (cat. #SML0965), and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were from Sigma-Aldrich (St. Louis, MO). Opti-MEM was from Gibco Life Technologies (Carlsbad, CA).

Szilágyi S.S., Amsalem-Zafran A.R., Shapira K.E., Ehrlich M, & Henis Y.I. (2022). Competition between type I activin and BMP receptors for binding to ACVR2A regulates signaling to distinct Smad pathways. BMC Biology, 20, 50.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!