The setup is assembled from a combination of Thorlabs and custom parts. A 3D-printed lick port housing an LED, an infrared photodiode and corresponding infrared photosensor2 is mounted on an xyz stage (DT12XYZ/M, Thorlabs). The mouse sits on a 3D-printed3 , 4 rectangular, walled stage mounted on a lab jack (S63081, Fisher). Water delivery for reward and facial air puff are controlled by fast solenoid valves (LHDA0531115H, Lee Company). We use the open source Bpod behavioral control system (Sanworks5 ) for real-time behavioral control and monitor the animals through infrared cameras (FL3-U3-13S2M-CS, Point Grey) using Bonsai open source computer vision software6 (Lopes et al., 2015 (link)) (Figures 3 , 4 ).
Dt12xyz m
Manufactured by Thorlabs
The DT12XYZ/M is a translation stage from Thorlabs. It is designed to provide precise linear motion along the X, Y, and Z axes. The stage features a compact size and is suitable for a variety of applications that require accurate positioning and alignment of optical components or other lab equipment.
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Lab products found in correlation
3 protocols using dt12xyz m
1
Mouse Behavioral Monitoring Setup
2
Electrical Shock Stimulation in Flies
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A rectangle of stacked copper plates was brought into contact with the fly’s abdomen and legs using a manually movable stage (DT12XYZ/M, Thorlabs). The design of the copper plates was based on Felsenberg et al., 2018 (link). Shocks were provided by a DS3 Constant Current Isolated Stimulator (1.2 s, 32 mA, Digitimer; maximum voltage 90 V) and triggered by the same software as the odor delivery. Successful shock delivery was confirmed by observing the fly’s physical reaction with a Genie Nano-M1280 camera (Teledyne Dalsa) coupled to a 1x lens with working distance 67 mm (SE-16SM1, CCS).
3
Electrical Stimulation of Fly Abdomen
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A rectangle of stacked copper plates was brought into contact with the fly's abdomen using a manually movable stage (DT12XYZ/M, Thorlabs). The design of the copper plates was based on (Felsenberg et al., 2018) . Shocks were provided by a DS3
Constant Current Isolated Stimulator (1.2 s, 32 mA, Digitimer; maximum voltage 90 V) and triggered by the same software as the odor delivery. Successful shock delivery was confirmed by observing the fly's physical reaction with a Genie Nano-M1280 camera (Teledyne Dalsa) coupled to a 1x lens with working distance 67 mm (SE-16SM1, CCS).
Constant Current Isolated Stimulator (1.2 s, 32 mA, Digitimer; maximum voltage 90 V) and triggered by the same software as the odor delivery. Successful shock delivery was confirmed by observing the fly's physical reaction with a Genie Nano-M1280 camera (Teledyne Dalsa) coupled to a 1x lens with working distance 67 mm (SE-16SM1, CCS).
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