Cd8 buv395 clone rpa t8

Manufactured by BD

Sourced in United States

CD8-BUV395 is a fluorochrome-conjugated monoclonal antibody that binds to the CD8 surface marker. CD8 is a glycoprotein expressed on the surface of certain T cells and NK cells. The BUV395 fluorochrome allows for detection and analysis of CD8-positive cells using flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Cd4 apc fire750 clone sk3, by BioLegend (2 mentions) Human trustain fcx, by BioLegend (2 mentions) Lsrfortessa, by BD (2 mentions) Anti human cd3 bv785, by BioLegend (1 mentions) Cd56 bv510, by BioLegend (1 mentions) Cd38 buv737, by BD (1 mentions) Cd73 pe clone ad2, by BioLegend (1 mentions) Cd45ra bv711 clone hi100, by BD (1 mentions) Cd8 fitc clone sk1, by BD (1 mentions) Efluor 506, by Thermo Fisher Scientific (1 mentions) Il 10 pe cy7 clone jes3 9d7, by Thermo Fisher Scientific (1 mentions) Cell stimulation cocktail, by Thermo Fisher Scientific (1 mentions) Cd19 apc cy7 hib19, by BioLegend (1 mentions) Fortessa x20, by BD (1 mentions) Anti human cd3 apc cy7 clone hit 3a, by BioLegend (1 mentions) Live dead cell aqua blue viability, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Roche (1 mentions) Cd4 apc h7 clone l200, by BD (1 mentions) Cd8 fitc clone hit8a, by BD (1 mentions)

Close spelling variants of this product

BUV395-anti-CD8 (clone RPA-T8, BUV395-conjugated anti-CD8 (clone RPA-T8, CD8-BUV395 (RPA-T8, CD8 (clone RPA-T8, Clone RPA-T8 RPA-T8 CD8-BUV395

5 protocols using cd8 buv395 clone rpa t8

Ectonucleotidase Expression on T-cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols

The expression of ectonucleotidases (CD39 and CD73) on αβDNT and activated Treg cells was evaluated by flow cytometry. The gating strategy for activated Treg cells was performed as previously described (5 (link)). PBMCs were stained with directly conjugated antibodies for 30 min at 4°C in the dark. The cells were washed before flow cytometry analysis. Antibodies used included anti-human CD3-BV785 (clone SK7), CD4-APC-fire750 (clone SK3), αβTCR-BV421 (clone IP26), CD56-BV510 (clone HCD56), CCR7 (CD197)-PE-cy7 (clone G043H7), HLA-DR-AF700 (clone L243), CD73-PE (clone AD2), CD39-BV605 (clone A1, BioLegend, San Diego, CA, USA), CD45RA-BV711 (clone HI100), CD38-BUV737 (clone HB7), CD25-PE-CF594 (clone M-A251), CD8-FITC (clone SK1), CD8-BUV395 (clone RPA-T8, BD Biosciences, San Diego, CA, USA), and the corresponding isotype controls. Data were acquired on a BD LSR Fortessa flow cytometer (BD Biosciences) and analyzed with FlowJo software (Tree Star, Ashland, OR, USA).

Wang X., Zhang L., Du J., Wei Y., Wang D., Song C., Chen D., Li B., Jiang M., Zhang M., Zhao H, & Kong Y. (2022). Decreased CD73+ Double-Negative T Cells and Elevated Level of Soluble CD73 Correlated With and Predicted Poor Immune Reconstitution in HIV-Infected Patients After Antiretroviral Therapy. Frontiers in Immunology, 13, 869286.

+ Open protocol

+ Expand

Flow Cytometric Analysis of IL-10 Expression in Stimulated PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

PBMCs, isolated as described above, were resuspended to 2×10⁶ cells/mL in RPMI 1640 medium plus 10% FBS (R10). Cells were then incubated for 5h at 37°C in a medium with 1 × Cell Stimulation Cocktail (containing 81 nM PMA and 1.34 nM ionomycin plus protein transport inhibitors, Ebioscience). Following incubation, the cells were washed and surface-stained with CD3-BV786 (clone SK7), CD8-BUV395 (clone RPA-T8, BD Biosciences), CD4-APC-fire750 (clone SK3, BioLegend) for 30 minutes in the dark at room temperature, followed by fixation and permeabilization. After permeabilization, cells were stained with IL-10-PE-cy7 (clone JES3-9D7, Ebioscience) antibodies for 50 minutes in the dark at room temperature. A fixable viability dye eFluor 506 (Ebioscience) was used to assess cell viability. Following staining, cells were washed and acquired on an LSRFortessa.

Zhang L., Wei Y., Wang D., Du J., Wang X., Li B., Jiang M., Zhang M., Chen N., Deng M., Song C., Chen D., Wu L., Xiao J., Liang H., Zhao H, & Kong Y. (2022). Elevated Foxp3+ double-negative T cells are associated with disease progression during HIV infection. Frontiers in Immunology, 13, 947647.

+ Open protocol

+ Expand

Comparative PBMC Analysis by Mass and Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

For comparison of lineage frequencies between Mass Cytometry and Flow Cytometry, 1 × 10⁶ PBMCs from the reference sample were stained with LIVE/DEADV® Fixable Blue Stain (1:2,000, Thermo Fisher). Fc receptors were blocked in 50 μL volume (Human TruStain FcX, BioLegend) for 5 min at room temperature immediately before adding 50 μL of surface antibody cocktail, which included CD3 BV605 (clone OKT3; BioLegend), CD8 BUV395 (clone RPA-T8; BD Biosciences), CD14 BV510 (clone M5E2; BioLegend), and CD19 APC-Cy7 (HIB19; BioLegend). After 20 min incubation at room temperature, excess antibody was washed out with Flow buffer (PBS + 1% FBS) and the cells were fixed in 2% PFA for 15 min at room temperature. Samples were acquired with a 5-laser LSRFortessa, equipped with following lasers: 488 nm, 561 nm, 640 nm, 405 nm, and 355 nm (BD Biosciences).

Kleinsteuber K., Corleis B., Rashidi N., Nchinda N., Lisanti A., Cho J.L., Medoff B.D., Kwon D, & Walker B.D. (2016). Standardization and Quality Control for High-Dimensional Mass Cytometry Studies of Human Samples. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 89(10), 903-913.

+ Open protocol

+ Expand

Profiling Tumor-Infiltrating Lymphocytes in NSCLC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lung cancer biopsies were obtained from non-treated patients with non-small-cell lung carcinoma who underwent a lobectomy at the thoracic surgery department of European Georges Pompidou Hospital. Briefly, biopsies were digested for 45 min at 37°C with DNAse I (30 IU/mL, Roche) and collagenase type IV (1 mg/mL, Life Technologies/Thermofisher), then filtered through a 70 µm strainer washed with PBS–FCS 2%. Flow cytometry analysis of tumor-infiltrating lymphocytes was performed. : Receptors for the Fc region (FcRs) were first blocked with Human TruStain FcX (Biolegend) 5 min at RT, then stained with anti-human CD3 APC-CY7 (clone HIT-3a, Biolegend), CD8 BUV395 (clone RPA-T8, BD Biosciences), CD103 Percpcy5.5 (clone Ber-ACT8, Biolegend), CD49a PE (clone TS2-7, Biolegend), PD1 BV650 (clone EH12.2H7, Biolegend), CXCR6 biotin (clone K041E5, Biolegend) and steptavidin BV711 (Biolegend). All cells were labeled using the live/dead cell aqua blue viability (Life Technologies). Acquisitions were performed on BD Fortessa X20 (Becton Dickinson), and data were analyzed on live singulet cells with FlowJo Software (BD).

Karaki S., Blanc C., Tran T., Galy-Fauroux I., Mougel A., Dransart E., Anson M., Tanchot C., Paolini L., Gruel N., Gibault L., Lepimpec-Barhes F., Fabre E., Benhamouda N., Badoual C., Damotte D., Donnadieu E., Kobold S., Mami-Chouaib F., Golub R., Johannes L, & Tartour E. (2021). CXCR6 deficiency impairs cancer vaccine efficacy and CD8+ resident memory T-cell recruitment in head and neck and lung tumors. Journal for Immunotherapy of Cancer, 9(3), e001948.

+ Open protocol

+ Expand

Monoclonal Antibody Staining Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal human-reactive mouse IgG2B α-CLEC5A Ab clone #283834, used for functional assays, and the corresponding isotype control (ctrl) clone #20116 were purchased from R&D Systems, USA. For FACS staining, a PE-labelled Ab clone #283834 was used together with its corresponding isotype ctrl, both obtained from R&D Systems. Other monoclonal Abs used in the manuscript for FACS staining are as follows: HLA-DR, DP, DQ BB515 clone Tu39, mouse IgG2ak BB515 clone G155-178, CD83 BUV737 clone HB15e, mouse IgG1k BUV737 clone X40, CD80 APC-R700 clone L307.4, mouse IgG1k APC-R700 clone X40, CD86 BV650 clone 2331, mouse IgG1k BV650 clone X40, CD163 Alexa647 clone GHI/61, mouse IgG1k Alexa647 clone MOP-C21, CD206 BUV395 clone 19.2, mouse IgG1k BUV395 clone X40, CD14 PE clone M5E2, mouse IgG2ak PE clone G155-178, CD274 (PDL1) BB515 clone MIH1, mouse IgG1k BB515 clone X40, TREM1 BV421 clone 6B1, mouse IgG1k BV421 clone X40, CD14 APC-H7 clone MφP9, CD16 BUV395 clone 3G8, CD64 PerCP-Cy5.5 clone 10.1, CD11c BV650 clone B-ly6, CD4 APC-H7 clone L200, CD4 APC clone RPA-T8, CD8 BUV395 clone RPA-T8, CD8 FITC clone HIT8a, CD56 Alexa 488 clone B159, and CD19 BV605 clone SJ25C1 purchased from BD Biosciences, USA. TREM2 PE clone 237820 and Rat IgG2b PE clone 141945 were purchased from R&D Systems.

Tosiek M.J., Groesser K., Pekcec A., Zwirek M., Murugesan G, & Borges E. (2022). Activation of the Innate Immune Checkpoint CLEC5A on Myeloid Cells in the Absence of Danger Signals Modulates Macrophages' Function but Does Not Trigger the Adaptive T Cell Immune Response. Journal of Immunology Research, 2022, 9926305.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!