Pe cy7 anti mouse cd8a

4T1, H22, HepG2, NIH‐3T3, and Panc02 were purchased from BeNa Culture Collection, China. Anti‐mouse PD‐1 antibody (PD‐1 Ab, BE0146‐100 mg) was purchased from Bio X Cell. Anti‐human PD‐1 Ab was obtained as a gift from Livzon Pharmaceutical Group Inc., China. Flow cytometry antibodies included PE anti‐mouse CD274 (B7‐H1, PD‐L1) (124308), APC/Cyanine7 anti‐mouse CD45 (103132), FITC anti‐mouse CD3 (100204), Brilliant Violet 421 anti‐mouse CD4 (100563), PE/Cy7 anti‐mouse CD8a (100722), PE anti‐mouse IFN‐γ (505808), Alexa Fluor 647 anti‐human/mouse Granzyme B (515406), APC anti‐mouse Ki‐67 (652406), PE anti‐mouse/human CD44 (103008), PE anti‐mouse Ly‐6G/Ly‐6C (Gr‐1) (108407), APC/Cyanine7 anti‐mouse/human CD11b (101226), Brilliant Violet 421 anti‐mouse F4/80 (123137), PE anti‐mouse CD86 (105008), PE/Cy7 anti‐mouse CD206 (MMR) (141720), Alexa Fluor 647 anti‐mouse FOXP3 (126408), APC anti‐mouse CD49b (pan‐NK cells) (108910), PE anti‐mouse CD25 (102008), PE anti‐mouse/human CD44 (103008), APC anti‐mouse CD62L (104412), Zombie Aqua Fixable Viability Kit (423102), Cell Activation Cocktail (with Brefeldin A) (423304), True‐Nuclear Transcription Factor Buffer Set (424401), and Intracellular Staining Permeabilization Wash Buffer (10X) (421002) were purchased from BioLegend.

Antibodies used included rabbit anti-human PD-L1 (#13684, Cell Signaling Technology, Beverly, MA, USA; 1:1000 for Western blot), anti-Cleaved Caspase-3 (#9664, Cell Signaling Technology; 1:250 for IHC), Rabbit anti-human AhR (#83200, Cell Signaling Technology; 1:50 for ChIP), goat anti-mouse PD-L1 (#AF1019, R&D, Minneapolis, MN, USA; 10 µg/mL for IHC), anti-Ki67 (#ab15580, Abcam, Cambridge, MA, USA; 1:500 for IHC), TTF1 (#ab76013, Abcam, Cambridge, MA, USA; 1:200 for IHC), anti-β-Actin (#A1978, Sigma, St. Louis, MO, USA; 1:5000 for WB), APC anti-mouse CD3 (#100235, Biolegend, San Diego, CA, USA; 1:20 for flow cytometry), PE/Cy7 anti-mouse CD8a (#100721, Biolegend; 1:20 for flow cytometry), APC/Cy7 anti-mouse CD4 (#100413, Biolegend; 1:20 for flow cytometry), PE anti-mouse CD45 (#103105, Biolegend; 1:20 for flow cytometry), PE/Cy7 anti-mouse PD-L1 (#124313, Biolegend; 1:20 for flow cytometry), PE anti-mouse PD-1 (#135206, Biolegend; 1:20 for flow cytometry), FITC anti-mouse B220 (#103205, Biolegend, San Diego, CA, USA; 1:20 for flow cytometry), In Vivo Plus anti-mouse PD-L1 (#BP0101), In Vivo Plus Rat IgG 2b Isotype Control (Clone: LTF-2, #BP0090) were purchased from BioXcell (West Lebanon, NH, USA). Benzo(a)pyrene (#B1760) and Alpha-Naphthoflavone (ANF; # N5757) were purchased from Sigma.