Log In Sign up
The largest database of trusted experimental protocols

Anti hamster cy3

Manufactured by Jackson ImmunoResearch
Visit Supplier

Anti-hamster Cy3 is a fluorescent dye conjugated to an antibody specific to hamster IgG. It is designed for use in immunoassays and other applications requiring the detection of hamster immunoglobulins.

Automatically generated - may contain errors

Lab products found in correlation

Anti rabbit cy5, by Jackson ImmunoResearch (3 mentions) Oct compound, by Electron Microscopy Sciences (2 mentions) Anti rat dylight649, by Jackson ImmunoResearch (2 mentions) Fluoroshield, by Electron Microscopy Sciences (2 mentions) A1 inverted confocal microscope, by Nikon (2 mentions) Target retrieval solution, by Agilent Technologies (2 mentions) S1699, by Agilent Technologies (2 mentions) Bx51 microscope, by Olympus (2 mentions) Ab3786, by Developmental Studies Hybridoma Bank (2 mentions) Anti t1α, by Developmental Studies Hybridoma Bank (2 mentions) Evos fl auto 2 imaging system, by Thermo Fisher Scientific (1 mentions) Anti rabbit af488, by Jackson ImmunoResearch (1 mentions) Evos fl auto microscope, by Thermo Fisher Scientific (1 mentions) Pna fluorescein, by Vector Laboratories (1 mentions) Hrp conjugated secondary antibody, by GE Healthcare (1 mentions) Gapdh, by Merck Group (1 mentions) Anti gm130, by BD (1 mentions) Anti gm130, by Abcam (1 mentions) Anti ha, by Fortrea (1 mentions)

Spelling variants of this product

Cy3-conjugated anti-hamster IgG (4 citations) Cy3-conjugated goat anti-Armenian hamster IgG (2 citations) Cy3-goat anti-Armenian hamster (2 citations) FITC -conjugated goat anti-hamster and cy3- conjugated anti-rabbit IgG (2 citations)

5 protocols using anti hamster cy3

1

Spleen Tissue Immunofluorescence Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were embedded in OCT compound (Electron Microscopy Sciences), frozen and sectioned at a thickness of 4 μm. Sections were then fixed in 75% methanol/25% acetone for 10 min at −20°C. After blocking, sections were incubated with appropriate dilutions of primary Abs (eBioscience) overnight at 4°C. Secondary anti-mouse AF488, anti-rabbit AF488, anti-rabbit Cy5, anti-rat DyLight649, or anti-hamster Cy3 (Jackson Immunoresearch) Abs were incubated on the slides for 2 h at 25°C the following day. Slides were mounted with Fluoroshield (Electron Microscopy Sciences) and subsequently imaged on an EVOS FL Auto 2 Imaging System (Life Technologies).
Ghosh D., Brown S.L, & Stumhofer J.S. (2017). IL-17 promotes differentiation of splenic LSK− lymphoid progenitors into B cells following Plasmodium yoelii infection. Journal of immunology (Baltimore, Md. : 1950), 199(5), 1783-1795.
+ Open protocol
+ Expand
2

Immunofluorescence Staining of Splenic Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were embedded and frozen in OCT compound (Electron Microscopy Sciences) prior to sectioning. Four micron splenic sections were then fixed in 75% ethanol/25% methanol for 10 mins at −20°C. After blocking, primary Abs CD3e and IgD (eBioscience) were applied for 4 hours at 25°C or overnight at 4°C. PNA-Fluorescein (Vector Labs), anti-rat DyLight649, and anti-hamster Cy3 (Jackson ImmunoResearch) secondary Abs were then applied for 1 hour at 25°C. Slides were mounted with Fluoroshield (Electron Microscopy Sciences) and subsequently imaged on a Cannon Ti-U fluorescent microscope. Images were acquired with a D5-QilMc digital camera in conjunction with NIS Elements software. For GC counts, staining was performed as above and images were captured on an EVOS FL Auto microscope (Life Technologies).
Wikenheiser D.J., Ghosh D., Kennedy B, & Stumhofer J.S. (2015). The Costimulatory Molecule ICOS Regulates Host Th1 and Follicular Th Cell Differentiation in Response to Plasmodium chabaudi chabaudi AS Infection. The Journal of Immunology Author Choice, 196(2), 778-791.
+ Open protocol
+ Expand
3

Histopathological Scoring of Lung Injury in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lungs were fixed in 4% formaldehyde overnight, and 3-μm paraffin-embedded sections were stained with hematoxylin and eosin (H&E). All images were obtained on an Olympus BX-51 microscope. To assess lung injury, slides were scored in a blinded fashion on a 12 point scale which addressed various aspects of histopathology including alveolar congestion, hemorrhage, presence of neutrophils in the airspaces, and degree of interstitial thickening (0 – 3 points each, based on severity).
For fluorescence imaging of paraffin embedded lung sections, Sections (4 μm) were cut, deparaffinized, and hydrated. Slides were incubated in Target Retrieval Solution (Dako #S1699) for 30 m in a pressure cooker, cooled to RT, and blocked in 5% goat / 5% donkey serum in tris buffered saline with 0.05% Tween (TTBS). Sections were then incubated with anti-pro-SPC (Millipore #AB3786) at 1:500 and anti-T1α (University of Iowa Developmental Studies Hybridoma Bank #8.1.1) at 1:100 overnight at 4°C. After washing in TTBS, sections were incubated with anti-rabbit Cy5 (Jackson #711-605-152) and anti-hamster Cy3 (Jackson #107166–142) antibodies for 1 h at RT, followed by DAPI for 10 m. Images were captures on a Nikon A1 inverted confocal microscope at 10X, serial images were tiled together to form a composite image of the entire lung section.
Gurczynski S.J., Nathani N., Warheit-Niemi H.I., Hult E.M., Podsiad A., Deng J., Zemans R.L., Bhan U, & Moore B.B. (2018). CCR2 mediates increased susceptibility to post-H1N1 bacterial pneumonia by limiting dendritic cell induction of IL-17. Mucosal immunology, 12(2), 518-530.
+ Open protocol
+ Expand
4

Comprehensive Antibody List for Autophagy Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies used in this work are as follows. Mouse antibodies: anti-WIPI2 (Polson et al., 2010 (link)), anti-GM130 (BD Biosciences; 610822), anti-PI4KΙΙα (Santa Cruz; sc-390026), anti-GFP (CRUK; 4E12), anti-HA (Covance; MMS-101R), anti-PI4P (Echelon Bioscience; Z-P004), GAPDH (Millipore; MAB374), and Syntaxin 12/13 (SYSY; 110131). Rabbit antibodies: anti-ATG9A (STO215 and STO219; Young et al., 2006 (link)), anti-LC3 (Abcam; ab48394), anti-GM130 (Abcam; ab52649), anti-ULK1 (for Western blotting, Santa Cruz; sc-33182; for immunofluorescence, Cell Signaling; 8054 D8H5), anti-GABARAP (Abgent; AP1821a), anti-Actin (Abcam; ab8227), anti-ARFIP2 (Invitrogen; 40-2400), anti-HA (Covance; PRB-101P), anti-PI4KIIIβ (Upstate; 06578), anti-pS294 PI4KIIIβ (Hausser et al., 2005 (link)), and anti-ATG13 (Chan et al., 2009 (link)). The following antibodies were used: hamster antibody: anti-ATG9A (Webber and Tooze, 2010 (link)); sheep antibody: anti-TGN46 (Serotec, AHP500G); goat antibody: anti-ARFIP1 (clone I-19; Santa Cruz; sc-19246).
Secondary antibodies for immunofluorescence (from Life Technologies unless otherwise specified) were anti-rabbit IgG Alexa Fluor 488, 555, and 647; anti-mouse IgG Alexa Fluor 488, 555, and 647; and anti-HAmster Cy3 (Jackson ImmunoResearch). HRP-conjugated secondary antibodies used for Western blotting were from GE Healthcare.
Judith D., Jefferies H.B., Boeing S., Frith D., Snijders A.P, & Tooze S.A. (2019). ATG9A shapes the forming autophagosome through Arfaptin 2 and phosphatidylinositol 4-kinase IIIβ. The Journal of Cell Biology, 218(5), 1634-1652.
+ Open protocol
+ Expand
5

Histopathological Scoring of Lung Injury in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lungs were fixed in 4% formaldehyde overnight, and 3-μm paraffin-embedded sections were stained with hematoxylin and eosin (H&E). All images were obtained on an Olympus BX-51 microscope. To assess lung injury, slides were scored in a blinded fashion on a 12 point scale which addressed various aspects of histopathology including alveolar congestion, hemorrhage, presence of neutrophils in the airspaces, and degree of interstitial thickening (0 – 3 points each, based on severity).
For fluorescence imaging of paraffin embedded lung sections, Sections (4 μm) were cut, deparaffinized, and hydrated. Slides were incubated in Target Retrieval Solution (Dako #S1699) for 30 m in a pressure cooker, cooled to RT, and blocked in 5% goat / 5% donkey serum in tris buffered saline with 0.05% Tween (TTBS). Sections were then incubated with anti-pro-SPC (Millipore #AB3786) at 1:500 and anti-T1α (University of Iowa Developmental Studies Hybridoma Bank #8.1.1) at 1:100 overnight at 4°C. After washing in TTBS, sections were incubated with anti-rabbit Cy5 (Jackson #711-605-152) and anti-hamster Cy3 (Jackson #107166–142) antibodies for 1 h at RT, followed by DAPI for 10 m. Images were captures on a Nikon A1 inverted confocal microscope at 10X, serial images were tiled together to form a composite image of the entire lung section.
Gurczynski S.J., Nathani N., Warheit-Niemi H.I., Hult E.M., Podsiad A., Deng J., Zemans R.L., Bhan U, & Moore B.B. (2018). CCR2 mediates increased susceptibility to post-H1N1 bacterial pneumonia by limiting dendritic cell induction of IL-17. Mucosal immunology, 12(2), 518-530.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!