Raddeanin A was purchased from Shanghai Yuanye Biotechnology, Ltd (Shanghai, China). Stock solution (20 mmol/L) was prepared by dissolution in DMSO and stored in the dark at −20°C. GSH (glutathione, γ‐glutamyl cysteinyl glycine), cycloheximide (CHX), SP600125 (JNK inhibitor), and NSC74859 (STAT3 inhibitor, S3I‐201) were purchased from Sigma‐Aldrich (St Louis, MO, USA). LY3214996 (ERK1/2 inhibitor) and Q‐VD‐OPh (caspase inhibitor) were purchased from MCE (Monmouth Junction, NJ, USA). Antibodies against STAT3, phospho‐STAT3S727, Bcl‐2, Bcl‐xl, Bax, total‐ and cleaved PARP, cleaved caspase‐3, cleaved caspase‐8, cleaved caspase‐9, c‐Jun, phospho‐c‐Jun, phospho‐JNK, JNK, ERK1/2, phospho‐ERK1/2 and actin were purchased from Cell Signaling Technology (Beverly, MA, USA).
Nsc74859
Manufactured by Merck Group
Sourced in United States
NSC74859 is a small molecule compound. It is commonly used in research laboratories for scientific experiments and analyses. The core function of NSC74859 is to serve as a research tool, but further details on its specific applications or intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
Cleaved caspase 3, by Cell Signaling Technology (2 mentions)
Erk1 2, by Cell Signaling Technology (2 mentions)
Cleaved caspase 9, by Cell Signaling Technology (2 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Phospho stat3 s727, by Cell Signaling Technology (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
Phospho c jun, by Cell Signaling Technology (1 mentions)
Cleaved caspase 8, by Cell Signaling Technology (1 mentions)
C jun, by Cell Signaling Technology (1 mentions)
Cleaved parp, by Cell Signaling Technology (1 mentions)
Phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)
Trizol reagent, by Merck Group (1 mentions)
Propidium iodide, by Thermo Fisher Scientific (1 mentions)
Mg132, by Merck Group (1 mentions)
Ly294002, by Merck Group (1 mentions)
Stattic, by Merck Group (1 mentions)
2 protocols using nsc74859
1
Raddeanin A-induced Apoptosis Mechanisms
2
Molecular Mechanisms of Apoptosis Regulation
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Propidium iodide (PI) was obtained from Molecular Probes (Carlsbad, CA, USA). TRIzol reagent, LY294002, wortmannin, PD98059, U0126, Stattic, NSC74859, PDTC, cyclohexamide (CHX), MG132, 2-DG, 17-AAG and chetomin were obtained from Sigma (St. Louis, MO, USA). Imiquimod was obtained from InvivoGen (San Diego, CA, USA). Antibodies specific to phospho-STAT3, STAT3, cleaved-caspase 3, cleaved-caspase 9, cleaved-PARP, HIF-1α, phospho-Ser473 Akt, Akt, phospho-ERK1/2 (Thr202/Tyr204) and ERK1/2 were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies specific to Mcl-1 and β-actin were purchased from Santa Cruz (Santa Cruz, CA, USA).
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