P5091 (P005091) was purchased from Selleck (Shanghai, People’s Republic of China). Dulbecco’s Modified Eagle’s Medium and fetal bovine serum were from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies against FOXP3, PARP, and cleaved-caspase-3 were purchased from Cell Signaling Technology (Danvers, MA, USA) and β-actin was bought from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). The TUNEL assay kit was bought from Vazyme Biotech Co., Ltd (Nanjing, People’s Republic of China). CD8-FITC (FITC-conjugated anti-CD8 antibody), FOXP3-PE (PE-conjugated anti-FOXP3 antibody), and IFN-γ-APC (APC conjugated anti-IFN-γ antibody) were purchase from BioLegend (San Diego, CA, USA). TRIzol reagent and cell culture products were obtained from Takara (Shiga, Japan).
Apc conjugated anti ifn γ antibody
Manufactured by BioLegend
Sourced in United States
The APC conjugated anti-IFN-γ antibody is a lab equipment product that detects and binds to interferon gamma (IFN-γ) proteins. The antibody is conjugated with the fluorescent dye Allophycocyanin (APC), which enables visualization and quantification of IFN-γ in samples through flow cytometry and other immunoassay techniques.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Ifn γ apc, by BioLegend (1 mentions)
Trizol reagent, by Takara Bio (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Tunel assay kit, by Vazyme (1 mentions)
Permeabilization buffer, by Thermo Fisher Scientific (1 mentions)
Pe conjugated anti cd3, by BioLegend (1 mentions)
Fitc conjugated anti cd45, by BioLegend (1 mentions)
Facscelesta, by BD (1 mentions)
2 protocols using apc conjugated anti ifn γ antibody
1
Immunomodulatory Effects of P5091 Compound
2
IFN-γ Production in NK Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To detect IFN-γ production by NK cells, cells were stimulated for 4 h with 5 μg/ml Brefeldin A (Yeasen, 50502ES03), then stained with FITC-conjugated anti-CD45 (Biolegend, 147710, dilution 1:200), PE-conjugated anti-CD3 (Biolegend, 100205, dilution 1:200), and BV421-conjugated anti-NK1.1 (Biolegend, 108741, dilution 1:200) antibodies. The cells were then fixed with 4% paraformaldehyde and permeabilized using permeabilization buffer (Invitrogen, 88-8824-00). Finally, the cells were stained with APC-conjugated anti-IFN-γ antibody (Biolegend, 505810, dilution 1:100). Data were acquired on a cytometer (FACSCelesta, BD Biosciences) and analyzed using FlowJo X software (Tree Star, Ashland, OR).
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