Fitc anti cd8 clone 53 6

Manufactured by BioLegend

Sourced in United States

The FITC anti-CD8 (clone 53-6.7) is a fluorescently-labeled antibody that binds to the CD8 receptor on the surface of T cells. It is a tool for identifying and quantifying CD8+ T cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Cytoflex flow cytometer, by Beckman Coulter (1 mentions) Anti cd3 clone ucht1, by Thermo Fisher Scientific (1 mentions) Ag85a, by BEI Resources (1 mentions) γ irradiated m tb h37rv, by BEI Resources (1 mentions)

Close spelling variants of this product

Anti-CD8-FITC (clone 53.6.7; CD8 FITC (clone 53‐6.7, Anti-CD8 (clone 53-6.7, CD8 (clone 53-6.7, CD8 (53-6.7, Clone 53-6.7 Anti-CD8-FITC CD8-FITC Anti-CD8

2 protocols using fitc anti cd8 clone 53 6

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

After harvesting and washing with 1× PBS, cells were stained with PerCP anti-CD3 (clone 145-2C11, BioLegend, Cambridge, MA, USA), PE anti-mouse CD4 (clone 4B12, Biolegend), FITC anti-CD8 (clone 53-6.7, BioLegend), APC anti-PD-L1 (clone 10F.9G2, BioLegend), PE/Cy7 anti-CD25 (clone PC61, BioLegend), PE anti-FoxP3 (clone 150D, BioLegend), Pacific Blue anti-NK1.1 (clone PK136, BioLegend), APC anti-Granzyme B (clone QA16A02, BioLegend), APC/Cy7 anti-CD107a (clone 1D4B, BioLegend), APC/Cy7 anti-KLRG-1 (clone 2F1/KLRG-1, BioLegend), APC anti-IFN-γ (clone XMG1.2, BioLegend). After staining, cells were analyzed by a CytoFLEX flow cytometer (Beckman Coulter, Indianapolis, IN, USA) and then percentage of gated cells was calculated using FlowJo_V10 software (FlowJo, LLC, Ashland, OR, USA).

Daneshmandi S., Wegiel B, & Seth P. (2019). Blockade of Lactate Dehydrogenase-A (LDH-A) Improves Efficacy of Anti-Programmed Cell Death-1 (PD-1) Therapy in Melanoma. Cancers, 11(4), 450.

+ Open protocol

+ Expand

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

FITC anti-CD4 (clone GK1.5, Biolegend), FITC anti-CD8 (Clone 53–6.7, Biolegend), FITC anti-CD14 (Clone 61D3, eBiosciences), qdot605 anti-CD14 (clone Tük4, Invitrogen), V450 anti-CD2 (clone RPA-2.10, eBioscience), anti-CD3 (clone UCHT1, eBioscience), anti-CD19 (clone HIB19, eBioscience), anti-CD20 (clone 2H7, eBioscience), anti-CD56 (clone B159, eBioscience), PE-Cy7 anti-CD16 (clone 3G8, Biolegend), APC-Cy7 anti-HLA-DR (clone L243, BD biosciences), PE anti-CD142/TF (clone HTF-1,eBioscience) and PerCP-Cy5.5 anti-TNF-α (clone MAb11, eBioscience) were used for flow cytometry, γ-irradiated M. tb H37Rv and Ag85a was obtained from BEI Resources.

Venkatasubramanian S., Tripathi D., Tucker T., Paidipally P., Cheekatla S., Welch E., Raghunath A., Jeffers A., Tvinnereim A.R., Schechter M.E., Andrade B.B., Mackman N., Idell S, & Vankayalapati R. (2015). TISSUE FACTOR EXPRESSION BY MYELOID CELLS CONTRIBUTES TO PROTECTIVE IMMUNE RESPONSE AGAINST Mycobacterium tuberculosis INFECTION. European journal of immunology, 46(2), 464-479.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!