Hc pl apochromat objective

Manufactured by Leica

The HC PL-APOCHROMAT objective is a high-performance microscope objective designed for advanced microscopy applications. It features an apochromatic lens design that provides superior color correction and high-resolution imaging across a wide field of view.

Automatically generated - may contain errors

Lab products found in correlation

Dfc365 fx digital camera, by Leica (2 mentions) Dm6000b microscope, by Leica (2 mentions) Application suite las af microscope software, by Leica (1 mentions) H2dcfda, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

HC PL APO 63x/1.40 OIL CS2 plan apochromat oil immersion objective

2 protocols using hc pl apochromat objective

Intracellular Hydrogen Peroxide Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following exposure to PL, cells were stained with 10 μM of the dye dihydrodichlorofluorescein-diacetate (H2DCFDA, Invitrogen Inc., Eugene, OR, USA) in 1 ml media to measure intracellular hydrogen peroxide. Cells were stained for 30 min at 37°C.
Fluorescence microscopy was performed on a Leica DM6000B Microscope, equipped with a Leica DFC365 FX digital camera. Images were captured using the Leica Application Suite (LAS-AF) microscope software. Representative pictures were taken using a 40x/0,85 Leica HC PL-APOCHROMAT objective.

Allaman-Pillet N, & Schorderet D.F. (2021). Piperlongumine promotes death of retinoblastoma cancer cells. Oncotarget, 12(9), 907-916.

+ Open protocol

+ Expand

Fluorescence Microscopy of Retinal Layers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescence microscopy was performed on a Leica DM6000B Microscope, equipped with a Leica DFC365 FX digital camera. Images were captured using the Leica Application Suite (LAS-AF) microscope software. Representative pictures were taken from central areas of the retina using a 40x/0,85 Leica HC PL-APOCHROMAT objective.
For quantifications of INL/ONL thickness, nuclei counting and TUNEL assays, pictures were captured in the posterior pole of the retina, once ventrally and once dorsally to the optic nerve head. Three sagittal sections from three different animals for each genotype and age were analyzed.

Allaman-Pillet N., Oberson A., Bustamante M., Tasinato A., Hummler E, & Schorderet D.F. (2015). Tgfbi/Bigh3 silencing activates ERK in mouse retina. Experimental eye research, 140.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!