The following reagents were obtained: ketamine (Dopalen injectable®, Paulínia, Brazil), xylazine (Anasedan injectable®, Paulínia, Brazil), Tween 80 (Synth®, Diadema, SP, Brazil), hexadecyltrimethylammonium bromide (HTAB) (Sigma Aldrich® H5882, Steinheim, Germany), o-dianisidine dihydrochloride (Sigma Aldrich® D3252, Steinheim, Germany), dextran sulphate sodium salt (DSS)—colitis grade (36,000–50,000 MW, cod. 02160110-CF, MP Biomedicals, LLC, Solon, OH, USA), hydrogen peroxide (Synth®), horseradish peroxidase (Sigma Aldrich® P8250, Steinheim, Germany), trichloroacetic acid (TCA) (Sigma Aldrich® T4885, Steinheim, Germany), reduced L-glutathione (Sigma Aldrich® G-4251, Steinheim, Germany), β-nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate (NADPH) (Sigma Aldrich® N-7505, Steinheim, Germany), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB) (Sigma Aldrich® D-8130, Steinheim, Germany), and buffer RIPA (Sigma Aldrich® R0278, Steinheim, Germany). IL-1β mouse, IL-10 mouse, CXCL-MIP-2 mouse, and TNF-α mouse were obtained from R&D Systems®, Inc. (Minneapolis, MN, USA).
D3252
Manufactured by Merck Group
Sourced in Germany, Switzerland
D3252 is a laboratory equipment product from Merck Group. It is designed for general laboratory use. The core function of D3252 is to provide a reliable and consistent platform for various laboratory tasks and experiments.
Automatically generated - may contain errors
Lab products found in correlation
H5882, by Merck Group (2 mentions)
R0278, by Merck Group (1 mentions)
D8130, by Merck Group (1 mentions)
G4251, by Merck Group (1 mentions)
N7505, by Merck Group (1 mentions)
P8250, by Merck Group (1 mentions)
T4885, by Merck Group (1 mentions)
Gentlemacs octo dissociator, by Miltenyi Biotec (1 mentions)
2 protocols using d3252
1
Murine Colitis Induction and Analysis
2
Enzymatic Activity Quantification of Colon and Small Bowel
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Both colon and small bowel specimens were homogenized in 50 mM phosphate buffer containing 0.5% hexadecyltrimethylammonium bromide (H-5882, Sigma) with the gentleMACS octo dissociator (130-095-937, Miltenyi, program MPO). After three freeze-and-thaw cycles, homogenates were centrifuged for 10 min at maximum speed. 20 mL of the supernatant were transferred to a 96-well plate in duplicate and mixed with 280 μL of 0.02% dianisidine (in 50 mM phosphate buffer and 0.0005% H2O2, D-3252, Sigma, St Gallen, Switzerland). Absorbance was measured at 460 nm after 20 min or 60 min for samples from Smad7 deficient and WT mice. Protein concentration of the supernatant was determined using the BCA Protein Assay Kit (23252, C Schuler et al.
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