P27 sc 1641

Manufactured by Santa Cruz Biotechnology

Sourced in United States

P27 (sc-1641) is a laboratory reagent produced by Santa Cruz Biotechnology. It is a primary antibody that can be used to detect the p27 protein in various applications such as Western blotting, immunohistochemistry, and flow cytometry. The core function of P27 (sc-1641) is to facilitate the identification and analysis of the p27 protein in biological samples.

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3 protocols using p27 sc 1641

Butein and Cisplatin Signaling Pathways

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Butein and cisplatin (CDDP) were obtained from Sigma (St. Louis, MO, USA), and were dissolved in dimethyl sulfoxide (DMSO) and stored at −20°C until use. The ERK inhibitor, U0126, p38 inhibitor, SB203580, and the AKT inhibitor, LY294002, were obtained from Sigma and used at final concentrations of 10, 20 and 20 µM, respectively. The following antibodies were used in western blot analysis: anti-ERK1/2 (#9102; 1:1,000), anti-p-ERK1/2 (Thr202/Tyr204) (#9101; 1:1,000), anti-p38 (#9212; 1:1,000), anti-p-p38 (Thr180/Tyr182) (#9211; 1:1,000), anti-Akt (#9272; 1:1,000) and anti-p-Akt (Ser413) (#9271; 1:1,000) (all purchased from Cell Signaling Technology, Danvers, MA, USA). β-actin (sc-4778; 1:5,000), forkhead box O3a (FoxO3 or FoxO3a; sc-9812; 1:1,000), Bax (sc-7480; 1:1,000), Bcl-2 (sc-7382; 1:1,000), p27 (sc-1641; 1:1,000) and cyclin D1 (sc-718; 1:1,000) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase (HRP)-conjugated goat anti-mouse/anti-rabbit immunoglobulin (IgG; ab6721; 1:1,000) was obtained from Abcam (Hong Kong, China).

ZHANG L., YANG X., LI X., LI C., ZHAO L., ZHOU Y, & HOU H. (2015). Butein sensitizes HeLa cells to cisplatin through the AKT and ERK/p38 MAPK pathways by targeting FoxO3a. International Journal of Molecular Medicine, 36(4), 957-966.

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Regulation of ITCH by CDK4/RXRα

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The HA-CCDC68 plasmid, a set of shRNA plasmids specifically targeting ITCH, and the Flag-RXRα plasmid were purchased from MiaoLingBio (Wuhan, China). The pEGFP-CDK4 plasmid was constructed as described in our previous study (18 (link)). The luciferase reporter driven by the ITCH promoter was constructed by inserting the ITCH promotor sequence into the pGL3-basic vector (E1751; Promega) with the primers (F) 5′;-TCTATCGATAGGTACCTGACTTTCCAGATGGCAAAATACT-3′; and (R) 5′;-CCGGAATGCCAAGCTTTTCGCCCACGGGGGTTTA-3′;. Antibodies against CDK2 (sc-6248), CDK4 (sc-260), CDK6 (sc-177), cyclinD1 (sc-20044), P21 (sc-397), and P27 (sc-1641) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against CDC37 (4793S), cyclin E2 (4132S), HA (3724S), BTRC (11984S), and RXRα (3085S) were purchased from Cell Signaling Technology (Boston, MA, USA). Antibodies against α-Tubulin (ab7291), FBXO4 (ab230302), and SOCS7 (ab224589) were purchased from Abcam (Cambridge, UK). Antibodies against CCDC68 (PA5-61687) and FZR1 (34-2000) were purchased from Invitrogen (Grand Island, NY, USA). Antibody against ITCH (20920-1-AP) was purchased from Proteintech (Chicago, IL, USA). The chemicals cycloheximide (CHX) and MG132 were purchased from Calbiochem (San Diego, CA, USA).

Wang C., Li H., Wu L., Jiao X., Jin Z., Zhu Y., Fang Z., Zhang X., Huang H, & Zhao L. (2021). Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation. Frontiers in Oncology, 11, 668743.

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Antibody-Based Protein Detection

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Western blot was performed as a routine way. GLS1 antibody was purchased from Abcam (ab93434). P21(sc-6246) and P27(sc-1641) were purchased from Santa Cruz. Antibodies against MYC (RT1149), KRAS (ER40115), GFP (ET1604-26), β-actin (M1210-2), and GAPDH (EM1101) were purchased from Hangzhou Huaan Biotechnology (Hangzhou, China).

Qiu Q., Li M., Yang L., Tang M., Zheng L., Wang F., Qiu H., Liang C., Li N., Yi D., Yi Y., Pan C., Yang S., Chen L, & Hu Y. (2019). Targeting glutaminase1 and synergizing with clinical drugs achieved more promising antitumor activity on multiple myeloma. Oncotarget, 10(57), 5993-6005.

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