Frozen serum samples of the study participants were thawed and used for biochemical tests. Serum glucose concentrations were measured by using the hexokinase method (Roche Diagnostics, Mannheim, Germany) with a Hitachi 7600 clinical chemistry analyzer (Hitachi Ltd., Tokyo, Japan). Total cholesterol (TC) and triglyceride (TG) concentrations were determined by using enzymatic methods (Sekisui, Tokyo, Japan and Roche Diagnostics, respectively), and HDL-C and LDL-C were determined by using a direct enzymatic procedure (Sekisui) with a Hitachi 7600 analyzer. Serum sdLDL-C concentrations were measured by using a homogeneous enzymatic assay (Denka Seiken, Tokyo, Japan) with a Hitachi-7600 analyzer. Serum insulin concentration was determined by using an automated enzyme chemiluminescence immunoassay (DxI; Beckman Coulter, Brea, CA, USA). As a surrogate index of insulin resistance, homeostasis model assessment of insulin resistance (HOMA-IR) was calculated by using the formula of Matthews et al. [21 (link)] as follows: fasting plasma insulin (mIU/L)×fasting plasma glucose (mmol/L)/22.5.
7600 analyzer
Manufactured by Hitachi
Sourced in Japan, Switzerland
The Hitachi 7600 analyzer is a high-performance clinical chemistry analyzer designed for laboratory use. It is capable of performing a wide range of biochemical tests and analyses on various biological samples. The core function of the Hitachi 7600 analyzer is to accurately measure and analyze the levels of different chemical and biological components in patient samples, providing healthcare professionals with the data necessary for diagnosis and treatment decisions.
Automatically generated - may contain errors
Lab products found in correlation
Hlc 723g7, by Tosoh (4 mentions)
Xe 2100 analyzer, by Sysmex (3 mentions)
Immulite 2000, by Siemens (2 mentions)
Variant 2, by Bio-Rad (2 mentions)
Glucose oxidase phenol 4 aminoantipyrine peroxidase kit, by Randox (2 mentions)
Integra 800 cts, by Roche (2 mentions)
Xn 10 hematology analyzer, by Sysmex (2 mentions)
Cica creatinine reagent, by Kanto Chemical (2 mentions)
Au5400, by Olympus (1 mentions)
Ca 7000 system coagulation analyzer, by Sysmex (1 mentions)
Advia centaur immunoassay system, by Siemens (1 mentions)
Modular e170, by Roche (1 mentions)
Spurr s resin, by Electron Microscopy Sciences (1 mentions)
Uc6 microtome, by Leica (1 mentions)
Cobas e601, by Roche (1 mentions)
Accu chek, by Roche (1 mentions)
Cobas integra 800, by Roche (1 mentions)
Accuri c6 flow cytometer, by BD (1 mentions)
Cobas taqman hbv test, by Roche (1 mentions)
Axsym, by Abbott (1 mentions)
75 protocols using 7600 analyzer
1
Comprehensive Serum Biomarker Analysis
2
Biomarkers in Burn Injury Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected via a central venous catheter and urine samples were collected via a Foley catheter, at 0, 3, 6, 12, 24, and 48 h after admission to the burn intensive care unit. The blood NGAL levels were measured with the Triage NGAL reagent and Triage Meter (Alere Healthcare, San Diego, CA, USA). The serum cystatin C levels were measured according to a turbidimetric immunoassay method with the HiSense cystatin C kit (HBi, Anyang, Korea) and Hitachi 7600 analyzer (Hitachi, Tokyo, Japan). The serum and urine creatinine levels were measured according to an enzymatic method with the Cica Creatinine reagent (KANTO Chemical, Tokyo, Japan) and Hitachi 7600 analyzer (Hitachi, Tokyo, Japan).
For the urine NGAL analysis, urine specimens were transferred to centrifuge tubes and centrifuged at a relative centrifugal force ≥ 400 for a minimum of 5 minutes; the supernatants were stored at -70°C prior to batch analysis. After thawing, the specimens were mixed and centrifuged at 2,500 to 3,000 × g for 10 minutes prior to use, to remove any particulate matter and ensure consistency in the results. The urine NGAL levels were measured in a chemiluminescence immunoassay with an Architect i2000SR analyzer (Abbott Diagnostics, Abbott Park, IL, USA) and a dedicated urine NGAL reagent (Abbott Diagnostics). All measurements were performed according to the manufacturers’ instructions.
For the urine NGAL analysis, urine specimens were transferred to centrifuge tubes and centrifuged at a relative centrifugal force ≥ 400 for a minimum of 5 minutes; the supernatants were stored at -70°C prior to batch analysis. After thawing, the specimens were mixed and centrifuged at 2,500 to 3,000 × g for 10 minutes prior to use, to remove any particulate matter and ensure consistency in the results. The urine NGAL levels were measured in a chemiluminescence immunoassay with an Architect i2000SR analyzer (Abbott Diagnostics, Abbott Park, IL, USA) and a dedicated urine NGAL reagent (Abbott Diagnostics). All measurements were performed according to the manufacturers’ instructions.
3
Quantifying Urinary Albumin in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urine was collected from individual mice housed in metabolic cages for 12 hours and was centrifuged at 2,000 g for 5 min. Urinary albumin concentrations were measured using a mouse albumin enzyme-linked immunosorbent assay (ELISA) kit (AKRAL-121; Shibayagi, Gunma, Japan) (19 (link)). Urinary creatinine concentration determination was conducted using the sarcosine oxidase PAP method on a Hitachi 7600 analyzer. The urine albumin-to-creatinine ratio (UACR) was detected during the experiments.
4
Comprehensive Metabolic Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Body weight, height, body mass index (BMI), blood pressure, glycated hemoglobin (HbA1c), and glycated albumin (GA) were determined by the method of Zhang et al.14 (link) Plasma glucose, SCr, blood UA, and blood UN were measured by an automatic analyzer (Olympus AU5400, Tokyo, Japan). UACR was calculated by dividing the urinary albumin concentrations measured with a Hitachi 7600 analyzer (Tokyo, Japan) by the urinary creatinine measured with chemiluminescence immunoassay (Siemens Immulite 2000; Siemens Healthcare GmbH, Erlangen, Germany).
5
Predictive Biomarkers in Liver Disease
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Demographic and laboratory values, including sex, age, total protein, albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin, creatinine, international normalized ratio (INR), D-dimer level, platelet count, and 30-day outcome, were derived from the institutional database. Biochemical values were measured using a Hitachi 7600 analyzer (Hitachi, Tokyo, Japan). Hematological parameters were measured using a Sysmex XE-2100 analyzer (Sysmex, Kobe, Japan). The D-dimer and INR were measured with a Sysmex CA1500 full-automatic analyzer (Sysmex Corp, Hyogo, Japan). The normal plasma D-dimer level at our hospital laboratory is less than 0.170 mg/L fibrinogen equivalent units (FEU). DAR was calculated as D-dimer level (mg/L) divided by albumin level (g/dL). At baseline, liver disease severity was assessed using the Model for End-Stage Liver Disease (MELD) score [22 (link)].
6
Precision Evaluation of POC Instruments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the precision of each POC instrument, within‐run coefficients of variation (CVs) were calculated using triplicate measurements of PSs. Passing‐Bablok regression analysis was used to estimate the slopes and intercepts of each of the POC instruments vs the Hitachi 7600 analyzer, and the Spearman rank correlation coefficient was also calculated.
7
Metabolic Assessment Protocol in Chinese Adults
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Medical history, height and weight were recorded. BMI was calculated as weight in kilograms divided by height in metres squared. A standard mercury sphygmomanometer was used to measure the blood pressure three times in the sitting position after 5 min rest; the average value was used for analysis.
Fasting antecubital venous blood was sampled to measure FPG. If FPG was ≥5.6 mmol/L, a standard 75 g glucose OGTT was performed within 8–10 hours. All measurements were performed in laboratories submitted to the quality control process of the Chinese Ministry of Health. A glucose oxidase method was used for the measurement of blood glucose. A Hitachi 7600 analyzer was used to detect creatinine, uric acid (UA) and blood lipids (total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C)). Creatinine clearance rate (CCR) (mL/min)=((140−age)×weight (kg))/(creatinine (μmol/L)×0.82 (men) or 0.85 (women)). All blood samples were centrally analysed within 24 hours.
Fasting antecubital venous blood was sampled to measure FPG. If FPG was ≥5.6 mmol/L, a standard 75 g glucose OGTT was performed within 8–10 hours. All measurements were performed in laboratories submitted to the quality control process of the Chinese Ministry of Health. A glucose oxidase method was used for the measurement of blood glucose. A Hitachi 7600 analyzer was used to detect creatinine, uric acid (UA) and blood lipids (total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C)). Creatinine clearance rate (CCR) (mL/min)=((140−age)×weight (kg))/(creatinine (μmol/L)×0.82 (men) or 0.85 (women)). All blood samples were centrally analysed within 24 hours.
8
KNHANES V: Comprehensive Health Data
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
KNHANES V consisted of a health interview survey, a health examination survey, and a nutrition survey. The data for the health interview and nutrition surveys were collected through individual interviews. Each participant’s serum was collected after overnight fasting. The samples were transported to the core laboratory and analyzed within 24 hours after collection. Serum glucose and serum GGT were analyzed using the Hitachi 7600 analyzer. Height was measured in units of 1 millimeter (mm), and body weight was measured in units of 0.1 kg using an automatic instrument.
9
Precise Serum Glucose Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum FSG concentrations were the primary outcome. FSG was measured with a COBAS INTEGRA 800 and a 7600 Analyzer (Hitachi, Tokyo, Japan). The FSG measurement followed internal and external quality control procedures, as required by the Korean Association of Laboratory Quality Control. Agreement for FSG across individual hospital was high (correlation coefficient range 0.96–0.99) [13 (link)].
10
Comparative Analysis of POC Instruments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PSs and the three EQA materials were measured with five POC instruments and the Hitachi 7600 analyzer on the same day. All samples were adequately mixed at room temperature before analysis and measured in triplicate; for the EQA materials, three replicates were performed on each instrument. Samples were evaluated by the instruments in a set order, and the elapsed time between the first and last measurements was <30 minutes. All measurements were performed in a laboratory setting with controlled room temperature (23 ± 5°C) and humidity, according to the manufacturers’ specifications.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!