hMDMs were prepared as indicated above and infected with GBS or unexposed to bacteria as a control. The hMDMs cell suspension was stained with Pe/Cy7 TNF anti-human antibody (BioLegend 502,929) or with antibodies for representative genes for the states: state 1- Brilliant Violet CXCR4 anti-human (BioLegend 306,517), state 3- Pe/Cy7 CD80 (BioLegend 305,217) and for state 5- APC IL7R anti-human (BioLegend 351,315) according to the BioLegend intracellular staining protocol. The bacterial fluorescence together with the marker staining was recorded on a FACS- SONY SH800 sorter and analyzed using FlowJo software v10.6.1. PE positive cells were considered infected with GBS. This experiment was done 3 times. Figures 2D –2F shows a representative experiment.
Brilliant violet cxcr4 anti human
Manufactured by BioLegend
The Brilliant Violet CXCR4 anti-human is a fluorochrome-conjugated antibody that binds to the CXCR4 chemokine receptor on human cells. It can be used for the identification and analysis of CXCR4-expressing cells in various applications, such as flow cytometry.
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Lab products found in correlation
2 protocols using brilliant violet cxcr4 anti human
1
Profiling Macrophage Response to Group B Streptococcus
2
Profiling Macrophage Response to Group B Streptococcus
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hMDMs were prepared as indicated above and infected with GBS or unexposed to bacteria as a control. The hMDMs cell suspension was stained with Pe/Cy7 TNF anti-human antibody (BioLegend 502,929) or with antibodies for representative genes for the states: state 1- Brilliant Violet CXCR4 anti-human (BioLegend 306,517), state 3- Pe/Cy7 CD80 (BioLegend 305,217) and for state 5- APC IL7R anti-human (BioLegend 351,315) according to the BioLegend intracellular staining protocol. The bacterial fluorescence together with the marker staining was recorded on a FACS- SONY SH800 sorter and analyzed using FlowJo software v10.6.1. PE positive cells were considered infected with GBS. This experiment was done 3 times. Figures 2D –2F shows a representative experiment.
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