Protein extracts from cells or tissues were prepared by homogenization in RIPA lysis buffer (Santa Cruz). Protein extracts were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membrane (Millipore). After incubation with the indicated primary antibodies at 4 °C overnight, the blots were incubated with IR Dye-coupled secondary antibodies (LI-COR) and visualized by the LI-COR Odyssey infrared imaging system. The anti-pSer473-Akt (#9271) and anti-Akt (#9272) antibodies were from Cell Signaling. The anti-Adiponectin antibody was from Dr. P.E. Scherer (UTSW).
Anti pser473 akt 9271
Manufactured by Cell Signaling Technology
The Anti-pSer473-Akt (#9271) is a lab equipment product that detects phosphorylation of Akt at serine 473. It is a primary antibody that can be used in various experimental techniques such as Western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti akt 9272, by Cell Signaling Technology (1 mentions)
Ir dye coupled secondary antibodies, by LI COR (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ripa lysis buffer, by Santa Cruz Biotechnology (1 mentions)
Polyvinylidene difluoride, by Merck Group (1 mentions)
Donkey anti mouse, by Jackson ImmunoResearch (1 mentions)
Acrylamide, by Bio-Rad (1 mentions)
Anti rabbit, by Jackson ImmunoResearch (1 mentions)
Magicmark, by Thermo Fisher Scientific (1 mentions)
Anti akt antibody, by Cell Signaling Technology (1 mentions)
2 protocols using anti pser473 akt 9271
1
Western Blot Analysis of Adiponectin
2
Phosphorylation of Na-K-ATPase α1 at Ser938
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Anti‐P‐Ser‐473 Akt (#9271) and anti‐AKT antibodies (#9272) were from Cell Signaling Technology. The phosphospecific antibody to the α1‐isoform of the Na‐K pump that is phosphorylated at Ser938 (anti‐P‐Ser938) of the rat Na‐K‐ATPase α1‐isoform was developed by Cell Signaling Technology and previously characterized (Massey et al., 2016 (link)). The antibody against the α‐subunit of sheep kidney Na+/K+ ‐ATPase (M8‐P1‐A3) was purchased from Sigma–Aldrich. Secondary antibodies were from Jackson ImmunoResearch: donkey anti‐mouse (# 715‐035‐150) and anti‐rabbit (# 711‐035‐152) both coupled to horseradish peroxidase (HRP). H‐89 was from LC Laboratories. SDS‐PAGE reagents were from Fisher Scientific (Hanover Park); acrylamide was from Bio‐Rad Laboratories; polyvinylidene difluoride was from Millipore; KPL chemiluminescence reagents were from Insight Biotechnology; phosphatase inhibitors (microcystin and okadaic acid) were from Axxora; the molecular weight marker MagicMark was from Invitrogen. CPT‐2Me‐cAMP (CPT) was from Tocris. All other reagents were from Sigma‐Aldrich.
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