Cyp3a5

IMB-YH-4py5-2H (purity>98%) was synthesized and purified by the Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences (Beijing, China). 3-(Dimethylamino)-1-phenyl-2-propen-1-one (Fig 1) was purchased from Sigma–Aldrich (Darmstadt, Germany) as an internal standard (IS, CAS: 1201-93-0, purity > 99%). HPLC-grade methanol and formic acid were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Ultrapure water was obtained from a Millipore system (Bedford, MA, USA). All other reagents were of analytical grade. Pooled human liver microsomes, NADPH-regenerating system, and recombinant P450 enzymes (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4 and CYP3A5) were purchased from Corning (Woburn, MA, USA).

Thirteen recombinant human CYP (rhCYP) isoforms, including CYP1A1, CYP1B1, CYP1A2, CYP3A4, CYP3A5, CYP2C8, CYP2B6, CYP2C9, CYP2D6, CYP2A6, CYP2C19, CYP2E1 and CYP4F2, were purchased from Corning Life Sciences (Corning, MA). Mixed liver microsomes from beagle dogs (DLMs), rats (RLMs), mini pigs (PLMs), monkeys (MLMs), mice (MIMs), rabbits (RAMs) and humans (HLMs) were obtained from the Research Institute for Liver Disease Co., Ltd. (Shanghai, China). All the liver microsomes and rhCYP were stored at −80 °C until use.