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Sirt7

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SIRT7 is a sirtuin family member that functions as a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase. It plays a role in the regulation of gene expression, cellular metabolism, and stress response pathways.

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Lab products found in correlation

Gapdh, by Cell Signaling Technology (4 mentions) Sirt1, by Cell Signaling Technology (3 mentions) Sirt3, by Cell Signaling Technology (3 mentions) Sirt2, by Cell Signaling Technology (3 mentions) Sirt5, by Cell Signaling Technology (3 mentions) Sirt6, by Cell Signaling Technology (3 mentions) Smad2 3, by Cell Signaling Technology (2 mentions) Odyssey scanner, by LI COR (2 mentions) Ripa lysis buffer, by Cell Signaling Technology (2 mentions) Mtco1, by Thermo Fisher Scientific (2 mentions) Image studio lite 5.0 analytical software, by LI COR (2 mentions) Sirt4, by Abcam (2 mentions) Total oxphos rodent wb antibody cocktail, by Abcam (2 mentions) β actin, by Thermo Fisher Scientific (2 mentions) Protein assay, by Bio-Rad (2 mentions) Chloroquine diphosphate, by Merck Group (1 mentions) Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions) Caspase 8, by Cell Signaling Technology (1 mentions) C caspase 9, by Santa Cruz Biotechnology (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-Sirt7 (6 citations) Anti-SIRT7 (5360), (2 citations)

7 protocols using sirt7

1

Autophagy Modulation and Sirtuin Signaling

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The following reagents were used in this study: tenovin-6 (BSCC-37, Agave Pharm, Seattle, WA), chloroquine diphosphate (C6628, Sigma, St. Louis, MO), bafilomycin A1 (B1793, Sigma), rapamycin (R8781, Sigma), and LysoTracker Red DND-99 (L7528, Thermo Fisher Scientific, Waltham, MA).
The antibodies used were: LC3B (CTB-LC3-1-50, Cosmo Bio, Carlsbad, CA), LC3A (ab62720, Abcam, Cambridge, MA), SQSTM1/p62 (PM045, MBL, New York, NY), ATG5 (#2630, Cell Signaling Technology, Danvers, MA), β-actin (sc-8432, Santa Cruz, Dallas, TX), β-tubulin (T8453, Sigma), p53 (DO-1, sc-126, Santa Cruz), acetyl-p53 (K382) (#2524, Cell Signaling Technology), Phospho-p53 (S15) (#9284, Cell Signaling Technology), Bax (#2772, Cell Signaling Technology), Puma (#4976, Cell Signaling Technology), p21 (#556430, BD Biosciences, Franklin Lakes, NJ), PARP-1 (#9532, Cell Signaling Technology), c-caspase 3 (#9664, Cell Signaling Technology), caspase 8 (#9746, Cell Signaling Technology), c-caspase 9 (SC-7885, Santa Cruz), SIRT1 (#8469, Cell Signaling Technology), SIRT2 (#12672, Cell Signaling Technology), SIRT3 (#2627, Cell Signaling Technology), SIRT4 (NB100-1406, Novus, St Charles, MO), SIRT5 (#8782, Cell Signaling Technology), SIRT6 (#2590, Cell Signaling Technology), and SIRT7 (#5360, Cell Signaling Technology).
Yuan H., He M., Cheng F., Bai R., da Silva S.R., Aguiar R.C, & Gao S.J. (2017). Tenovin-6 inhibits proliferation and survival of diffuse large B-cell lymphoma cells by blocking autophagy. Oncotarget, 8(9), 14912-14924.
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2

Western Blot Analysis of Extracellular Matrix Proteins

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The protein samples (30 μg per lane) were separated by 10% SDS-PAGE, transferred to a polyvinylidene fluoride membrane (PVDF, Millipore, USA), and then blocked with 5% nonfat milk at room temperature for 1 h. The membrane was incubated overnight at 4 °C with primary antibodies, including those against GAPDH (1:2000, Proteintech, China), CD9 (1:1000, Abcam, USA), CD81 (1:1000, Abcam, USA), elastin (1:1000, Abcam, USA), collagen I (1:1000, Abcam, USA), collagen III (1:1000, Abcam, USA), Smad2/3 (1:1000, Cell Signaling Technology, USA), p-Smad2/3 (1:1000, Cell Signaling Technology, USA), TGF-β1 (1:1000, Abcam, USA), and Sirt7 (1:1000, Cell Signaling Technology, USA). Next, the membrane was incubated with an HRP-conjugated antibody (1:4000, Servicebio, China) at room temperature for 1 h and detected using an ECL chemiluminescence detection kit (Servicebio, China) with a BioSpectrum 600 Imaging System (UVP, CA, USA). The band density was determined by the ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Zhang H., Huang J., Liu J., Li Y, & Gao Y. (2020). BMMSC-sEV-derived miR-328a-3p promotes ECM remodeling of damaged urethral sphincters via the Sirt7/TGFβ signaling pathway. Stem Cell Research & Therapy, 11, 286.
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3

Acetylation Analysis of Sirtuin Proteins

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The following primary antibodies were used: SIRT1 (ab104833; Abcam; 8469S; Cell Signaling Technology), SIRT6 (12486; Cell Signaling Technology), SIRT7 (5360; Cell Signaling Technology), β-actin (A5316; Sigma-Aldrich), FoxO1 (2880S; Cell Signaling Technology), Sp1 (sc-14027; Santa Cruz), CD25-APC (17-0259-42; Affymetrix eBioscience), and CD69-APC (310910; BioLegend). For mass spectrometry analysis, a total anti-acetyllysine antibody was used (ICP0388-2MG; ImmuneChem).
Jeng M.Y., Hull P.A., Fei M., Kwon H.S., Tsou C.L., Kasler H., Ng C.P., Gordon D.E., Johnson J., Krogan N., Verdin E, & Ott M. (2018). Metabolic reprogramming of human CD8+ memory T cells through loss of SIRT1. The Journal of Experimental Medicine, 215(1), 51-62.
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4

Molecular Mechanisms of Ang-II Signaling

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Ang-II and U0126 were purchased from R&D systems (Minneapolis, MN, USA). Sirt7, GAPDH, α-SMA, paxillin, phospho-Smad2, phospho-Smad3, Smad2/3, GFP and Vinculin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). LaminB, PAI-1, FN, P-Thr/Ser and Collagen I antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HRP-conjugated secondary antibodies were purchased from Santa Cruz Biotechnology.
Wang H., Liu S., Liu S., Wei W., Zhou X., Lin F., Wang J., Chen J., Zhang G, & Pang Y. (2017). Enhanced expression and phosphorylation of Sirt7 activates smad2 and ERK signaling and promotes the cardiac fibrosis differentiation upon angiotensin-II stimulation. PLoS ONE, 12(6), e0178530.
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5

Western Blotting of Cell Cycle Proteins

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Western blotting was performed with a SDS-PAGE Electrophoresis System, as described previously. 13 Primary antibodies against cyclin E, CDK2, Sirt7, GAPDH (Cell Signaling Technology, Danvers, MA, USA), cyclin D1, cyclin A, cyclin-dependent-kinase 4 (CDK4) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and tubulin (Calbiochem) were used.
Kimura Y., Izumiya Y., Araki S., Yamamura S., Hanatani S., Onoue Y., Ishida T., Arima Y., Nakamura T., Yamamoto E., Senokuchi T., Yoshizawa T., Sata M., Kim-Mitsuyama S., Nakagata N., Bober E., Braun T., Kaikita K., Yamagata K, & Tsujita K. (2021). Sirt7 Deficiency Attenuates Neointimal Formation Following Vascular Injury by Modulating Vascular Smooth Muscle Cell Proliferation. Circulation journal : official journal of the Japanese Circulation Society, 85(12).
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6

Quantitative Western Blotting of Sirtuins

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Cell were lysed with RIPA lysis buffer (Cat#9803, Cell signaling) plus 0.5 mM PMSF and Protein inhibitor tablets (Cat#A32963, Pierce TM , Thermo Scientific). The protein content of the lysate was determined using Bio-Rad Protein Assay (Bio-Rad) on a spectrophotometer. The same amount of isolated proteins were separated by SDS-PAGE in LI-COR running buffer with reduced reagent, and transferred to PVDF membrane. After the membrane was blocked with LI-COR blocking buffer, it was immunoblotted with primary antibody, including SIRT1 (Cat#2028), SIRT2 (Cat#D4S6J), SIRT3 (Cat#5490), SIRT5 (Cat#8779), SIRT6 (Cat#12486), SIRT7 (Cat#5360) or GAPDH (Cat#2118S) from Cell Signaling Technologies, or SIRT4 (ab124521) and Total OXPHOS Rodent WB Antibody Cocktail (ab110413) from Abcam, or β-actin (Cat#PA5-59497) and MTCO1 (Cat#459600) from ThermoFisher Scientific. Finally, Goat anti-rabbit or anti-mouse IRDye 680 or IRDye 800 secondary antibodies were used for the detection and quantitation of immunoblots. Membranes were imaged using a LI-COR Odyssey scanner, and blots were analyzed by Image Studio Lite 5.0 analytical software (LI-COR, Lincoln, NE) as previously described (Meruvu et al., 2016a; Zhang and Choudhury, 2017) .
Zhang J., Kay M.K., Park M.H., Meruvu S., Powell C, & Choudhury M. (2022). LncRNA DLEU2 regulates sirtuins and mitochondrial respiratory chain complex IV: a novel pathway in obesity and offspring's health. International journal of obesity (2005), 46(5).
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7

Quantitative Western Blotting of Sirtuins

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Cell were lysed with RIPA lysis buffer (Cat#9803, Cell signaling) plus 0.5 mM PMSF and Protein inhibitor tablets (Cat#A32963, Pierce TM , Thermo Scientific). The protein content of the lysate was determined using Bio-Rad Protein Assay (Bio-Rad) on a spectrophotometer. The same amount of isolated proteins were separated by SDS-PAGE in LI-COR running buffer with reduced reagent, and transferred to PVDF membrane. After the membrane was blocked with LI-COR blocking buffer, it was immunoblotted with primary antibody, including SIRT1 (Cat#2028), SIRT2 (Cat#D4S6J), SIRT3 (Cat#5490), SIRT5 (Cat#8779), SIRT6 (Cat#12486), SIRT7 (Cat#5360) or GAPDH (Cat#2118S) from Cell Signaling Technologies, or SIRT4 (ab124521) and Total OXPHOS Rodent WB Antibody Cocktail (ab110413) from Abcam, or β-actin (Cat#PA5-59497) and MTCO1 (Cat#459600) from ThermoFisher Scientific. Finally, Goat anti-rabbit or anti-mouse IRDye 680 or IRDye 800 secondary antibodies were used for the detection and quantitation of immunoblots. Membranes were imaged using a LI-COR Odyssey scanner, and blots were analyzed by Image Studio Lite 5.0 analytical software (LI-COR, Lincoln, NE) as previously described (Meruvu et al., 2016a; Zhang and Choudhury, 2017) .
Zhang J., Kay M., & Choudhury M. (2021). LncRNA DLEU2 regulates Sirtuins and mitochondrial respiratory chain complex IV: a novel pathway in obesity and DOHaD.
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