Mouse il 6 high sensitivity elisa

Total adiponectin was measured (n = 37 IL 10^tm, n = 33 controls) using the ALPCO (Mouse) Total Adiponectin ELISA (ALPCO Diagnostics, Salem, NH) using 10 μl samples in duplicate per the manufacturer’s instructions. The sensitivity of this assay was 0.032 ng/ml.
Plasma leptin was measured (n = 38 IL 10^tm, n = 33 controls) using the ALPCO Leptin ELISA (ALPCO Diagnostics, Salem, NH) using 30 μl samples in duplicate per the manufacturer’s instructions. The sensitivity of this assay was 10 pg/ml.
Plasma IL- 6 was measured (n = 37 IL 10^tm, n = 33 controls) using the mouse IL-6 High Sensitivity ELISA (eBioscience, Inc., Sandiego, CA) using 50 μl samples in duplicate per the manufacturer’s instructions. The sensitivity of this assay was 0.21 pg/ml.
Serum A1AT levels were measured (n = 21 IL 10^tm, n = 20 controls) with ELISA kits using 2 μl samples in duplicate according to the instructions provided by Immunology Consultants Laboratory (Newberg, OR). The sensitivity of this assay was 1.492 ng/ml.

Serum IL-6 concentrations were measured by Mouse IL-6 High Sensitivity ELISA according to the manufacturer’s protocol (eBioscience). Serum anti-GPI IgM and IgG antibodies were measured by ELISA according to a previously reported protocol with slight modifications39 (link). A serum mixture of 4 WT mice 14 days after immunization was used as 1000 unit (U) standard of the anti-GPI antibody. Serum anti-CCP2 antibodies were measured by Mesacup CCP2 test (MBL) in accordance to the previous study40 (link).