Cacl2 2h2o

D-glucose, L-glutamic acid, NH₄Cl, K₂HPO₄, MgSO₄·7 H₂O, CaCl₂·2 H₂O, MnSO₄·H₂O, FeCl₃·6 H₂O, ZnSO₄·7 H₂O, Na₂-EDTA, CuSO₄·5 H₂O, and CoCl₂·6 H₂O were purchased from Carl Roth GmbH+Co. KG (Karlsruhe, Germany). LC/MS-grade ultra-pure water, HPLC-grade chloroform, acetic acid, H₂SO₄, and NH₄HCO₃ were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 10-Camphorsulfonic acid and tributylamine were purchased from SigmaAldrich (MO, USA).

Calcium chloride dihydrate (CaCl₂∙2H₂O), di-potassium hydrogen phosphate trihydrate (K₂HPO₄∙3H₂O), magnesium sulphate heptahydrate (MgSO₄∙7H₂O), urea, zinc chloride (ZnCl₂) and yttrium standard solution Y(NO₃)₃ in HNO₃, 1000 mg/L Y) were obtained from Merck KGaA (Darmstadt, Germany). Sodium chloride (NaCl), methanol (LC-grade) and acetic acid (99%) were obtained from VWR Chemicals (Leuven, Belgium). Benzoic acid and benzotriazole were obtained from Carl Roth GmbH (Karlsruhe, Germany).
benzotriazole, Benzoic acid, urea, CaCl₂∙2H₂O, K₂HPO₄∙3H₂O, MgSO₄∙7H₂O, ZnCl₂ and NaCl were used for the production of synthetic PI-WW. acetic acid, methanol and Y(NO₃)₃ in HNO₃ were used for sample preparation and analysis.

Single-mode optical fibers (SMF-28e) were purchased from Corning Optical Communications (Berlin, Germany). Ethanol, glycerol, (3-glycidyloxypropyl)trimethoxysilane (GOPTS), L-cysteine, phosphate buffered saline (PBS; pH = 7.4), H₂O₂ (ω = 30%), potassium hydroxide, and urea were purchased from Sigma-Aldrich (Taufkirchen, Germany). Acetic acid, CaCl₂·2H₂O, 1 M HCl, MgCl₂·6H₂O, L-ascorbic acid, KCl, NaCl, 1 M NaOH, H₂SO₄ (ω = 96%) and Tris base were purchased from Carl-Roth (Karlsruhe, Germany). Hydrofluoric acid (HF; ω = 40%) and immersion oil were purchased from AppliChem (Darmstadt, Germany). Water was purified with a Milli-Q purification system. Human recombinant C-reactive protein (CRP; β = 1 mg/mL) was purchased from BioCat (Heidelberg, Germany) and pooled human >97% CRP deficient plasma was purchased from Dunn Labortechnik (Asbach, Germany). The CRP-specific single-stranded DNA aptamer (CRP-40-17-3′SH) with a thiol group at the 3′-end was synthesized and HPLC was purified by Metabion (Planegg, Germany) and delivered at 100 µM in bidest. water. The sequence of CRP-40-17-3′SH was: 5′-CCC CCG CGG GTC GGC TTG CCG TTC CGT TCG GCG CTT CCC CTT TTT TTT T-C6-SH-3′ [50 (link)].