Phosphate buffered saline (pbs)

Paraformaldehyde was purchased from Tianjin Chemical Reagent Research Institute (Tianjin, China). PBS was purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd (Beijing, China). Ammonia was purchased from Hangzhou Changzheng Chemical Plant (Hangzhou, China). Hydrogen peroxide was purchased from Shanghai Yuanda Peroxide Co., Ltd (Shanghai, China). Methanol was purchased from Shanghai Zhenxing Chemical No. 1 Plant (Shanghai, China). Anhydrous ethanol was purchased from Hangzhou Chemical Reagent Co., Ltd (Hangzhou, China). Ethanol (95%) was purchased from Anhui Ante Biochemical Co., Ltd (Suzhou, China). SHP was previously prepared, and the chemical constituents were identified using UPLC-MS/MS [11 (link)].

Ibrutinib was purchased from Macklin (Shanghai, China). Poly I:C was purchased from APExBIO (Houston, TX, USA). LPS was purchased from Sigma (St Louis, MO, USA). Mouse IL-1β, IL-6, and TNF-α ELISA kits were procured from Solaibao (Beijing, China). Anti-myeloperoxidase mouse mAb, anti-IL1 beta rabbit pAb, anti-TNF-α rabbit pAb, anti-CD8 mouse mAb, and anti-Ly6g rabbit pAb were obtained from Servicebio (Wuhan, China). APC Anti-Mouse Ly6G Antibody [1A8] and PerCP/Cyanine5.5 Anti-Mouse CD45 Antibody [30-F11] were purchased from Elabsciense (Shanghai, China). Antibodies against BTK/p-BTK (Tyr223), ERK/p-ERK, AKT/p-AKT, FLT3/p-FLT3, and EGFR/p-EGFR were bought from Cell Signaling Technology (Beverly, MA, USA). Antibodies against GAPDH, NFκB/p-NFκB, IL-1β, IL-6, IFN-γ, and TNF-α were purchased from Affinity Biosciences (Inc., Cincinnati, OH, USA). The RIPA lysis buffer and a Bradford protein assay kit were purchased from Biyuntian Biotechnology (Shanghai, China). PBS was purchased from Zhongshan Jinqiao Biotechnology (Co., Ltd., Beijing, China). A SuperLumia ECL HRP kit was procured from Abbkine (Redlands, CA, USA).