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Fitc mouse anti human cd61

Manufactured by BD
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Sourced in United States

The FITC mouse anti-human CD61 is a fluorescently labeled monoclonal antibody that binds to the CD61 antigen expressed on the surface of human cells. CD61 is a component of the integrin receptor, which plays a role in cell adhesion and signaling. This product can be used for the identification and enumeration of CD61-positive cells in various applications, such as flow cytometry.

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Lab products found in correlation

Mepacrine, by Merck Group (1 mentions) Isorhamnetin, by Cayman Chemical (1 mentions) Pe mouse igg1 isotype control, by BD (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions) Dihydroethidium dhe, by Merck Group (1 mentions) Fcs express, by De Novo Software (1 mentions) Pe mouse anti human cd29, by BD (1 mentions)

Close spelling variants of this product

Mouse anti-human CD61 Anti-CD61-FITC CD61-FITC Anti-CD61 Mouse anti

2 protocols using fitc mouse anti human cd61

1

Platelet activation and apoptosis assay

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Thrombin 6 receptor activating peptide (TRAP-6), collagen, and phorbol myristate acetate (PMA), were obtained from Sigma-Aldrich (St. Louis, MO, USA). Antimycin (AA), citrate-dextrose solution, mepacrine, dihydroethidium (DHE), intracellular calcium fluorescence indicator (Fluo-3-AM), and trifluoromethoxyphenylhydrazone (FCCP) were also obtained from Sigma-Aldrich (St. Louis, MO, USA). FITC annexin V apoptosis, PE mouse IgG1 isotype control, and FITC mouse anti-human CD61 were obtained from BD Biosciences (San Diego, CA, USA). Isorhamnetin was obtained from Cayman Chemical, USA. All assays incorporated as vehicle control dimethyl sulfoxide (DMSO) 0.2%.
Rodríguez L., Badimon L., Méndez D., Padró T., Vilahur G., Peña E., Carrasco B., Vogel H., Palomo I, & Fuentes E. (2021). Antiplatelet Activity of Isorhamnetin via Mitochondrial Regulation. Antioxidants, 10(5), 666.
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2

Flow Cytometry Analysis of CD61 and CD29

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Cells, treated with CT20p, were washed with 5% FBS in PBS and stained with FITC mouse anti-human CD61, PE mouse anti-human CD29 (BD Bioscience), or a corresponding isotype control antibody (BD Bioscience). Data were acquired with an Accuri C6 flow cytometer and analyzed with FCS Express (Denovo, Niwot, CO, USA) software.
Lee M.W., Bassiouni R., Sparrow N.A., Iketani A., Boohaker R.J., Moskowitz C., Vishnubhotla P., Khaled A.S., Oyer J., Copik A., Fernandez-Valle C., Perez J.M, & Khaled A.R. (2014). The CT20 peptide causes detachment and death of metastatic breast cancer cells by promoting mitochondrial aggregation and cytoskeletal disruption. Cell Death & Disease, 5(5), e1249-.
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