The hepatic specimens were fixed in 4% paraformaldehyde and embedded in paraffin. Liver sections were stained with hematoxylin-eosin or were subjected to Masson's trichrome stain and immunohistochemistry with antibodies against GFP (Abcam, Cambridge, UK), fibronectin (Abcam, Cambridge, UK), α-SMA (Santa Cruz, Dallas, USA), CK-7 (Abcam, Cambridge, UK), CK-19 (Proteintech, Chicago, USA) and Ki-67 (Abcam, Cambridge, UK).
Masson s trichrome stain
Manufactured by Abcam
Sourced in United Kingdom, United States
Masson's trichrome stain is a histological staining technique used to differentiate various types of connective tissues in biological samples. It primarily stains collagen fibers blue, while muscle fibers are stained red and nuclei are stained black.
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Lab products found in correlation
α sma, by Abcam (1 mentions)
Fibronectin, by Abcam (1 mentions)
Omnimount, by National Diagnostics (1 mentions)
Histo clear 2, by National Diagnostics (1 mentions)
H e staining, by Merck Group (1 mentions)
Goat anti mbp, by Santa Cruz Biotechnology (1 mentions)
Sc 13912, by Santa Cruz Biotechnology (1 mentions)
Mayer s hematoxylin, by Merck Group (1 mentions)
Thiazolyl blue tetrazolium bromide mtt, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
Glutathione (gsh), by Fujifilm (1 mentions)
2 2 2 tribromoethanol, by Merck Group (1 mentions)
Eosin y disodium salt, by Merck Group (1 mentions)
Superfrost plus glass slide, by Thermo Fisher Scientific (1 mentions)
Lumar v12, by Zeiss (1 mentions)
Matlab, by MathWorks (1 mentions)
Caseviewer software, by 3DHISTECH (1 mentions)
5 protocols using masson s trichrome stain
1
Liver histology and immunohistochemistry
2
Cryosectioning and Masson's Trichrome Staining
3
Histological Analysis of Sciatic Nerve
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For hematoxylin and eosin (H&E) staining, mice were sacrificed, and sciatic nerves were dissected and postfixed in 10% formalin for 24 h at 4°C. Sciatic nerves were cut into three segments: proximal, injury site, and distal. Samples were then dehydrated in ethanol followed by xylene and embedded in paraffin. Sciatic nerve segments were cut transversely into 4-µm sections using a microstat (Leica Biosystems). Sciatic nerve sections were deparaffinized with xylene and rehydrated through a graded ethanol series to water before H&E staining (Sigma-Aldrich). For MBP staining, the sections were subjected to pretreatment with EDTA, pH 9.0, for 30 min. Sections were then incubated overnight with the primary antibody goat anti-MBP (1:1,000; sc-13912; Santa Cruz Biotechnology, Inc.). Immunostaining was visualized by an avidin–biotin complex method, followed by light counterstaining with hematoxylin. For the axonal visualization, 10-µm sections were cut and stained according to Palmgren’s silver stain method. Staining with Masson’s trichrome stain was performed as per the manufacturer’s protocol (Abcam).
4
Antimicrobial Hydrogel Wound Dressing
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Pluronic F127 (PL), alginate (AL), sodium nitrite, Mayer’s hematoxylin, eosin-Y disodium salt, 2,2,2-tribromoethanol, tert-amyl alcohol (2-methyl-2-butanol) (Avertin anesthesia component), and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glutathione (GSH) was purchased from Wako Pure Chemical (Osaka, Japan). Methicillin-resistant Staphylococcus aureus 3089 (MRSA) and multidrug-resistant Pseudomonas aeruginosa 2200 (MRPA) were purchased from the Korea National Research Resource Bank (KNRRB, Seoul, Korea). BactoTM tryptic soy broth (TSB) and DifcoTM Luria Bertani (LB) media were purchased from BD Biosciences (Sparks, MD, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and penicillin–streptomycin were purchased from Hyclone (Thermo Fisher Scientifc Inc., Waltham, MA, USA). Masson’s trichrome stain (connective tissue stain) was purchased from Abcam (Cambridge, MA, USA). All other reagents and solvents were of analytical grade.
5
Tissue Staining and Digital Analysis
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Sections 1 and 4 from each of the four different types of tissue preparations were mounted on a Superfrost Plus glass slides (Fisher Scientific, 12–550-15). The slides were baked at 58 °C for 60 min and stained with Masson’s trichrome stain (Abcam, ab150686) according to the manufacturer instructions. The slides were digitally scanned with the Panoramic Flash 250 scanner (3DHistech, Budapest, Hungary), using 20x/0.8NA Zeiss objective or Zeiss Lumar v.12 stereomicroscope. The regions of interest (ROIs) were manually drawn on the scanned sections, using CaseViewer software (3DHistech, Budapest, Hungary), exported into .tiff files at full resolution (0.243 mm/pixel) and analyzed using ImageJ (NIH) or MATLAB (MathWorks).
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