Cell-cycle distribution was determined by flow cytometry according to the manufacturer’s instructions for the cell-cycle staining buffer (MultiSciences CCS01). Cells were detected with a FACSCalibur.
Ccs01
Manufactured by MultiSciences Biotech
Sourced in China
The CCS01 is a compact cell culture system designed for laboratory use. It provides a controlled environment for the cultivation of cells, ensuring consistent temperature, humidity, and atmospheric conditions. The device features precise monitoring and regulation of these parameters to support optimal cell growth and proliferation.
Automatically generated - may contain errors
4 protocols using ccs01
1
Cell Cycle Profiling by Flow Cytometry
2
Porcine Preadipocyte Proliferation Assay
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Porcine intramuscular preadipocytes being the stable phase of cell growth, cells were seeded in 6-well culture plates at a density of 1.6 × 106 cells per well. After 24 h, cells were transfected with miR-425-5p agomir/NC by X-tremeGENE HP siRNA Transfection Reagent (Roche), and cells were washed three times with PBS, harvested at 48 h post-transfection, and then stained with DNA staining solution (CCS01; Multi Sciences, Hangzhou, China), and left to incubate for 30 min. Finally, a flow cytometry instrument (Becton Dickinson, Franklin Lakes, NJ, USA) was used to analyze the proliferation phase of the cells.
3
Cell Cycle Analysis by Flow Cytometry
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Cell cycle was analyzed using a cell cycle staining kit (cat. no. CCS01, Multisciences (lianke) Biotech Co., Ltd., Hangzhou, China) according to the manufacturer’s instructions. Briefly, cells were harvested and washed with cold PBS twice, stained with binding buffer containing 50 μg/mL propidium iodide in the dark at room temperature for 30 min, and then immediately analyzed by flow cytometry (FACSCalibur flow cytometer; BD Biosciences, San Jose, CA, USA).
4
Cell Cycle Analysis by Flow Cytometry
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Approximately 2 × 105–1 × 106 cells were collected from the control or 250 μM AA2P-treated (36 h) groups, respectively, and then treated using the cell cycle staining kit (CCS01, MultiSciences Biotech Co., Ltd, Hangzhou, China) according to the manufacturer’s instruction. Briefly, cells were centrifuged and re-suspended in 1 mL DNA staining solution (propidium iodide) and 10 μL permeabilization solution. After vortexing for 5–10sec, cells were incubated in dark for 30min. Finally, cells were subjected to flow cytometry (BD AiraII, USA) to determine the stages of cell cycle.
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