Nu nu mice

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro

Nu/nu mice are a strain of mice that are homozygous for the nude (nu) gene mutation. This mutation results in the absence of a functional thymus gland, leading to a lack of T cells and a compromised immune system. These mice are often used in research as models for studying immunodeficiency and as hosts for xenograft studies.

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Lab products found in correlation

Matrigel, by Corning (4 mentions) Nod scid mice, by Jackson ImmunoResearch (2 mentions) Matrigel basement membrane matrix, by BD (2 mentions) Pbs matrigel, by BD (2 mentions) Matrigel, by BD (2 mentions) Xenogen ivis spectrum in vivo imaging system, by PerkinElmer (1 mentions) Estradiol, by Innovative Research (1 mentions) Facsaria 2, by BD (1 mentions) Recombinant human bfgf, by Merck Group (1 mentions) Growth factor reduced matrigel, by BD (1 mentions) Matrigel matrix, by BD (1 mentions) Matrigel, by Jackson ImmunoResearch (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions) Microtainer sst tube, by BD (1 mentions) Phosphate buffered saline (pbs), by Jackson ImmunoResearch (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Magnetic beads, by BioLegend (1 mentions) Balb c, by Jackson ImmunoResearch (1 mentions) C57bl 6, by Jackson ImmunoResearch (1 mentions) Gdc 0941, by Selleck Chemicals (1 mentions)

Close spelling variants of this product

Nu/J mice (66 citations) Nude nu/nu mice (17 citations) Athymic nude (nu/nu) mice (17 citations) Nu/J nude mice (10 citations) Female Nu/J mice (9 citations) Balb/c athymic nude mice (nu/nu) (8 citations) Athymic nude (NU/J) mice (8 citations) Female athymic nude mice (nu/nu, (7 citations) Athymic nu/nu female mice (4 citations) Foxn1 (nu/nu) mice (4 citations)

39 protocols using nu nu mice

Subcutaneous and Intraperitoneal GIST Xenograft Models

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Tumor xenograft models were created using the GIST-T1 cell line. Five million cells were injected subcutaneously into the right flank of 5- to 6-week-old nu/nu mice (The Jackson Laboratory, Bar Harbor, ME) and allowed to grow until tumor volume was 100–200 mm³. Mice were then euthanized and tumors were harvested for ex vivo analysis.
GFP-labelled GIST-T1 cells were also used to create an intraperitoneal xenograft GIST model. Five- to 6-week-old nu/nu mice (The Jackson Laboratory) were injected with 5 million cells intraperitoneally. Mice were monitored weekly for tumor growth by visual inspection and In Vivo Imaging System (IVIS) imaging as described below.

Banerjee S., Yoon H., Yebra M., Tang C.M., Gilardi M., Narayanan J.S., White R.R., Sicklick J.K, & Ray P. (2020). Anti-KIT DNA Aptamer for Targeted Labeling of Gastrointestinal Stromal Tumor. Molecular cancer therapeutics, 19(5), 1173-1182.

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Ultrasound-Guided Tumor Ablation in Mice

Cited 1 time

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All experiments were approved by the Institutional Animal Care and Use Committee of West Virginia University. Athymic female Nu/Nu mice were purchased from the Jackson Laboratory (JAX^®, Bar Harbor, ME, USA). All mice were approximately 25 g and 4-6 weeks old at the start of experimentation. Mice were anesthetized using 1.5-2% isoflurane. A custom animal restraint platform was constructed for repeatable placement and adjustment of mice on the FDA-approved ExAblate transducer, as outlined previously (18 (link)). The animals were placed on a rodent bed with an anesthesia mask. The baseplate was placed on the transducer, allowing positioning of the mouse in a supine position, with the skull immersed in degassed water. Vertical and horizontal adjustments were possible for tumor bearing mice based on animal size and weight. A single-channel MRI loop coil was fixed to the restraint for imaging. Animal body temperature during imaging and treatments was maintained using heating pads, and animals were monitored for any signs of distress.

Arsiwala T.A., Blethen K.E., Wolford C.P., Panchal D.M., Sprowls S.A., Fladeland R.A., Kielkowski B.N., Pritt T.A., Wang P., Wilson O., Carpenter J.S., Finomore V., Rezai A, & Lockman P.R. (2023). Blood-tumor barrier opening by MRI-guided transcranial focused ultrasound in a preclinical breast cancer brain metastasis model improves efficacy of combinatorial chemotherapy. Frontiers in Oncology, 13, 1104594.

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Breast Cancer Metastasis Imaging in Mice

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Luciferase expressing MDA-MB-231 cells were purchased from Perkin Elmer, Inc. NOD/Scid mice (001303; Jackson Laboratory, Inc.) or nu/nu mice (002019; Jackson Laboratory, Inc.) were used in this study. For the xenograft study of breast cancer metastasis, MDA-MB-231 breast cancer cells were exposed to mechanical strain or static conditions for 24 h. The cancer cells were either untreated or, treated with 10 μM of PI3-Kγ inhibitor (CAS 648450-29-7). We injected 5 × 10⁵ cells were injected into the tail vein of 7-week-old female mice. The mice were given an intraperitoneal injection of luciferin (150 mg/kg) in DPBS 10 min prior to imaging. The luminescence was visualized using the Xenogen IVIS Spectrum In Vivo Imaging System (PerkinElmer).

Spencer A., Sligar A.D., Chavarria D., Lee J., Choksi D., Patil N.P., Lee H., Veith A.P., Riley W.J., Desai S., Abbaspour A., Singeetham R, & Baker A.B. (2021). Biomechanical regulation of breast cancer metastasis and progression. Scientific Reports, 11, 9838.

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Xenograft Model of Neuroendocrine Tumors

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The Mycoplasma-free BON-Luc (3×10⁶ cells) were injected subcutaneously onto the flank of 4–6-week nude (nu/nu) mice (Jackson Labs, Bar Harbor, ME). The NET xenograft mice with tumor volume of 50–60 mm³ were randomized into 3 groups (n = 6): saline, anti-SSTR2 mAb, and mAb-MMAE conjugate. The mAb or ADC was administrated intravenously through tail vein following a dose of 8 mg/kg-BW (empirically determined from PK study) in 50 or 100 μL of saline. The same volume of mAb or saline was injected in control groups. Mice developed palpable nodules within 14 days and tumor volume and mouse body weight were measured every two days. Both electronic caliper and bioluminescence via In Vivo Imaging system (IVIS) were used to monitor tumor size. Four injections were conducted with average injection interval of 4.5 days during the entire treatment period (total of 24 days, our standard 3-week ADC treatment). At the end of the experiment, mice were euthanized to collect tumors and other organs (e.g. brain) for further analysis.

Si Y., Kim S., Ou J., Lu Y., Ernst P., Chen K., Whitt J., Carter A.M., Markert J.M., Bibb J.A., Chen H., Zhou L., Jaskula-Sztul R, & Liu X.“. (2020). Anti-SSTR2 Antibody-drug Conjugate for Neuroendocrine Tumor Therapy. Cancer gene therapy, 28(7-8), 799-812.

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Xenograft Tumor Development in Mice

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Tumor xenografts were developed by injecting the indicated amount of cells in 100% Matrigel Basement Membrane Matrix (BD Biosciences) into the fourth mammary fad pad of ovariectomized female NOD/SCID mice (for tumor growth) or nu/nu mice (for limiting dilution experiments) (Jackson Labs, Bar Harbor, ME, USA). Silastic pellets containing either 17β-estradiol alone (1 mg) or in combination with MPA (10 mg) were implanted subcutaneously at time of tumor cell injection. Tumors were measured weekly using a digital caliper, and tumor volume estimated using the formula (lw^2)/2. At termination of the experiment, mice were euthanized and tumors were excised and weighed. These experiments were performed under an approved University of Colorado Institutional Animal Care and Use Committee protocol.

Finlay-Schultz J., Cittelly D.M., Hendricks P., Patel P., Kabos P., Jacobsen B.M., Richer J.K, & Sartorius C.A. (2014). Progesterone downregulation of miR-141 contributes to expansion of stem-like breast cancer cells through maintenance of progesterone receptor and Stat5a. Oncogene, 34(28), 3676-3687.

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Xenograft Tumor Development in Mice

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Xenograft Tumor Development in nu/nu Mice

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Five, 6-8 week-old, female nu/nu mice were obtained from Jackson Laboratories (strain no. 002019). 3.5×10⁶ H58 cells suspended in 100 μL of culture media were injected into the flank of each mouse. Tumor volume and weight for each mouse was determined twice weekly for 20 weeks.

Felsenstein M., Trujillo M.A., Huang B., Nanda N., Jiang Z., Jeong Y.J., Pflüger M., Goggins M.G., Hruban R.H., Thompson E.D., Heaphy C.M., Roberts N.J, & Wood L.D. (2020). Generation and characterization of a cell line from an intraductal tubulopapillary neoplasm of the pancreas. Laboratory investigation; a journal of technical methods and pathology, 100(7), 1003-1013.

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Establishment of Orthotopic Breast Cancer Xenografts

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All procedures performed were approved by the Institutional Animal Care and Use Committee at Duke University. Cultured BT474, MDA-MB-453, or BT20 breast cancer cells were trypsinized at 80% confluence, washed with PBS, and resuspended in a 1:1 mixture of serum free medium and Matrigel (Corning Inc.). The cells (1–5 × 10⁶) were then orthotopically implanted into the axial mammary fat pads of 6-week old Nu/Nu mice (The Jackson Laboratory). Of note, 48 h prior to the injection of cells, mice receiving BT474 tumors were ovariectomized and implanted with estradiol (0.72 mg/60 days continuous release) treatment pellets (Innovative Research of America) to facilitate tumor initiation. Tumors dimensions were measured three times weekly with calipers, and body weight and behavior were assessed at the time of measurement. Tumor volume was calculated as A × B2 × 0.5, where A is the longer of the perpendicular axes. After reaching 0.5–1 cm³ volume (6–10 weeks depending on cell line), tumors were excised following humane euthanasia, cryopreserved and archived at -80 °C for later use.

Joh D.Y., Heggestad J.T., Zhang S., Anderson G.R., Bhattacharyya J., Wardell S.E., Wall S.A., Cheng A.B., Albarghouthi F., Liu J., Oshima S., Hucknall A.M., Hyslop T., Hall A.H., Wood K.C., Shelley Hwang E., Strickland K.C., Wei Q, & Chilkoti A. (2021). Cellphone enabled point-of-care assessment of breast tumor cytology and molecular HER2 expression from fine-needle aspirates. NPJ Breast Cancer, 7, 85.

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Doxorubicin Therapy in Xenograft Mouse Model

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Mouse experiments were conducted in the Harvard Medical School Animal Facility using protocols approved by the Harvard Medical School Institutional Animal Care and Use Committee (IACUC). 6-weeks-old male nu/nu mice (Jackson Laboratories) were subcutaneously inoculated in the flank with 3×10⁶ WT or SPARCLE KO HCT116 cells. Beginning fourteen days later, animals received 8 mg/kg doxorubicin (DOX) intraperitoneally weekly for four weeks. Tumor size was monitored every other day. Mice were sacrificed when any tumor in the experiment reached the maximal allowable size (100 mm³). Excised tumors were fixed in 4% formaldehyde for 24 hr at room temperature and embedded in paraffin.

Walter J., Hertel C., Tannock G.W., Lis C.M., Munro K, & Hammes W.P. (2001). Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella Species in Human Feces by Using Group-Specific PCR Primers and Denaturing Gradient Gel Electrophoresis. Applied and Environmental Microbiology, 67(6), 2578-2585.

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Xenograft Mouse Model for Cancer Research

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All procedures involving mice were approved and performed following the guidelines of the IACUC of Duke University Division of Laboratory Animal Resources. All studies employed 8–12-week old age-matched female outbred athymic nu/nu mice (#007850; RRID: IMSR_JAX:007850) purchased from The Jackson Laboratory. The mice were maintained under pathogen-free conditions in the Duke Cancer Center Isolation Facility for immune-deficient mice.

Luttman J.H., Hoj J.P., Lin K.H., Lin J., Gu J.J., Rouse C., Nichols A.G., MacIver N.J., Wood K.C, & Pendergast A.M. (2021). ABL allosteric inhibitors synergize with statins to enhance apoptosis of metastatic lung cancer cells. Cell reports, 37(4), 109880.

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