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SciTools is a suite of laboratory equipment designed for various research and analysis tasks. It provides a range of instruments and accessories to support scientific workflows. The core function of SciTools is to facilitate efficient and accurate data collection and processing in a laboratory setting.

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Lab products found in correlation

Sybr green supermix, by Bio-Rad (1 mentions) Rnaqueous micro kit, by Thermo Fisher Scientific (1 mentions) Biorad cdna synthesis kit, by Bio-Rad (1 mentions) Primer express software, by Thermo Fisher Scientific (1 mentions) Applied biosystems 7900ht sequence detection system, by Thermo Fisher Scientific (1 mentions) Oligofectamine, by Thermo Fisher Scientific (1 mentions) Iscript cdna synthesis kit, by Bio-Rad (1 mentions) Iq sybr green supermix, by Bio-Rad (1 mentions) Rneasy kit, by Qiagen (1 mentions)

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IDT SciTools (3 citations)

6 protocols using scitools

1

Tumor-infiltrating CD8+ T cell transcriptome

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Tumor-infiltrating CD8+ T cells were FACS sorted from E0771 tumor-bearing WT and S1pr1-Tg mice. RNA was extracted using column purification (Ambion RNAqueous Micro kit). cDNA was prepared using Bio-Rad cDNA synthesis kit (Bio-Rad). RT-PCR was performed using Bio-Rad SYBR Green Supermix (Bio-Rad). Primers were generated using SciTools (Integrated DNA Technologies) and PubMed blasted for gene and species specificity. Each primer set was validated using a standard curve across the dynamic range of interest with a single melting peak.
Priceman S.J., Shen S., Wang L., Deng J., Yue C., Kujawski M, & Yu H. (2014). S1PR1 is crucial for accumulation of regulatory T cells in tumors via STAT3. Cell reports, 6(6), 992-999.
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2

Quantifying PID1 mRNA Expression

Cited 1 time
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Quantitative real-time PCR was performed using the Applied Biosystems 7900HT sequence detection system (Applied Biosystems) as described7 (link). PCR primers and probes were designed and synthesized using Primer Express software (Applied Biosystems) or SciTools (Integrated DNA Technologies). Primers and probes, PID1: forward primer: 5′-AGCCAGTCATTGAGCTCTGGAAGA-3′, reverse primer: 5′-TGGTCGAGATGATGGAGCCAAACT-3′, probe: 5′-TTTCCGGCCAATGCCCTCCTGGAAAT-3′; GAPDH: forward primer: 5′-CAACTACAT GGTTTACATGTTCCAATATG-3′, reverse primer: 5′-GGGATCTCGCTCCTGGAAG-3′, probe: 5′-CGTTCTCAGCCTTGACGGTGCCA-3′. TaqMan real-time PCR data were analyzed using ABI Sequence Detector Software. PID1 mRNA was normalized to GAPDH mRNA, which was quantified in parallel in each sample.
Xu J., Ren X., Pathania A.S., Fernandez G.E., Tran A., Zhang Y., Moats R.A., Shackleford G.M, & Erdreich-Epstein A. (2017). PID1 increases chemotherapy-induced apoptosis in medulloblastoma and glioblastoma cells in a manner that involves NFκB. Scientific Reports, 7, 835.
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3

Codon-optimized Ppip5K for Yeast Expression

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Codon-optimisation of D. discoideum Ppip5K sequences for yeast expression was designed through an interface from SciTools® (Integrated DNA Technology). Restriction sites were added at the 5′ SalI and 3’ NotI to cloned Ppip5K in a pADH-GST plasmid (Azevedo et al., 2015 (link)).
Desfougères Y., Portela-Torres P., Qiu D., Livermore T.M., Harmel R.K., Borghi F., Jessen H.J., Fiedler D, & Saiardi A. (2022). The inositol pyrophosphate metabolism of Dictyostelium discoideum does not regulate inorganic polyphosphate (polyP) synthesis. Advances in Biological Regulation, 83, 100835.
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4

Caveolin-1 Knockdown Using siRNA

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Transfection was carried using Oligofectamine (Invitrogen, Carlsbad, CA) transfection reagent. Small interfering RNA (siRNA) was designed against the coding sequence of Cav-1 cDNA using software by scitools (Integrated DNA Technologies, Coralville, IA). CAV-1 siRNA sequence primers were forward, 5′-GCAUUAAGAGCUUCCUGAUUGAGAT-3′ and reverse, 5′-AUCUCAAUCAGGAAGCUCUUAAUGCAU-3′. Cells in 6-well plates at 50-60% confluency were incubated with 3 ml of culture media lacking antibiotics and containing 30 μl of Oligofectamine in the absence and presence (30 μM) of Cav-1 siRNA. After 6 h, the cells were washed in 1X PBS, and were cultured in new DMEM lacking fetal bovine serum. Cells were incubated at 37oC in a CO2 incubator for 48 h until ready to assay for gene knockdown.
Lee J.A., Choi D.I., Choi J.Y., Kim S.O., Cho K.A., Lee J.B., Yun S.J, & Lee S.C. (2014). Methyl-β-cyclodextrin up-regulates collagen I expression in chronologically-aged skin via its anti-caveolin-1 activity. Oncotarget, 6(4), 1942-1953.
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5

Generating BLEG-1 Variants for Expression

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The sequence of wild-type (WT) BLEG-1 retrieved from the National Library of Medicine (NCBI) (https://www.ncbi.nlm.nih.gov/protein/655553190, accessed on September 26th, 2021) was subjected to codon optimization for expression in Escherichia coli B series using Integrated DNA Technologies (IDT) SciTools (https://sg.idtdna.com/CodonOpt, accessed on October 15th, 2021). The codon optimized WT BLEG-1 sequence was used for the design of BLEG-1 variants, by substituting the existing codon with GCG which encodes alanine residue at the chosen positions. Following this, the genes of BLEG-1 variants were commercially synthesized and cloned into pUCIDT-Amp vector by IDT Inc., USA, giving forth pUCIDT-Amp::I10A, pUCIDT-Amp::F57A, pUCIDT-Amp::R94A, pUCIDT-Amp::L95A, and pUCIDT-Amp::R159A recombinant plasmids.
Au S.X., Mohd Padzil A., Muhd Noor N.D., Matsumura H., Raja Abdul Rahman R.N, & Normi Y.M. (2023). Probing the substrate binding modes and catalytic mechanisms of BLEG-1, a promiscuous B3 metallo-β-lactamase with glyoxalase II properties. PLOS ONE, 18(9), e0291012.
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6

Quantitative RT-PCR Expression Analysis

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Total RNA was prepared using RNeasy kit (Quiagen) following the manufacturer’s instructions. cDNA was prepared using iScript cDNA Synthesis Kit (Bio-Rad), and real-time PCR reactions were performed using iQ SYBR Green supermix (Bio-Rad) on CFX Connect equipped (Bio-Rad). Primers were generated using SciTools (Integrated DNA Technologies) and PubMed blasted for gene and species specificity. Each primer set was validated using a standard curve across the dynamic range of interest with a single melting peak. GAPDH housekeeping gene was used as internal control to normalize mRNA expression.
Lifshitz V., Priceman S.J., Li W., Cherryholmes G., Lee H., Makovski-Silverstein A., Borriello L., DeClerck Y.A, & Yu H. (2017). Sphingosine-1-Phosphate Receptor-1 Promotes Environment-Mediated and Acquired Chemoresistance. Molecular cancer therapeutics, 16(11), 2516-2527.
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