Proteins were extracted with RIPA lysis buffer containing 1 mM PMSF (Beyotime, Lianyungang, China). The protein samples were subjected to SDS-PAGE/immunoblotting analysis using anti-phospho-Smad1/5/8 antibody, anti-Runx2 antibody, anti-phospho- and anti-total ERK1/2 antibodies, anti-phospho- and anti-total p38 antibodies, anti-phospho- and anti-total JNK1/2 antibodies, and anti-GAPDH antibody (all from Cell Signaling, Danvers, MA, USA). The relative integrated density of each protein band was determined using an Odyssey infrared imaging system (LI-COR, Lincoln, NE, USA).
Anti phospho and anti total p38 antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-phospho- and anti-total p38 antibodies are laboratory reagents used to detect and quantify the p38 mitogen-activated protein kinase (MAPK) protein in cellular samples. The phospho-specific antibody detects p38 MAPK when it is phosphorylated, while the total antibody detects both phosphorylated and unphosphorylated forms of the protein.
Automatically generated - may contain errors
Lab products found in correlation
Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (2 mentions)
Anti phospho and anti total erk1 2 antibodies, by Cell Signaling Technology (2 mentions)
Anti phospho and anti total jnk1 2 antibodies, by Cell Signaling Technology (2 mentions)
Anti runx2 antibody, by Cell Signaling Technology (1 mentions)
Anti gapdh antibody, by Cell Signaling Technology (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
Anti phospho smad1 5 8 antibody, by Cell Signaling Technology (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
Anti β actin antibody, by Cell Signaling Technology (1 mentions)
2 protocols using anti phospho and anti total p38 antibodies
1
Protein Extraction and Immunoblotting Analysis
2
Jasminin Modulates MAPK Pathways
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RAW 264.7 cells (1 × 105 cells/well) were parallelly cultured in 24-well plates with 50 μM jasminin or inhibitors for 24 h, and the cell culture supernatants were then collected for TNF-α detection. The parallel cultured plates were treated with jasminin (50 μM) or inhibitors for 0–60 min, followed by cell lysis with RIPA buffer containing 1 mM PMSF (Beyotime Biotechnology, Shanghai, China). The extracted proteins were then subjected to SDS-PAGE/immunoblot analysis using antiphospho- and antitotal ERK1/2 antibodies, antiphospho- and antitotal p38 antibodies, antiphospho- and antitotal JNK1/2 antibodies, and anti-β-actin antibody (all from Cell Signaling Technology, Beverly, MA, USA). The integrated density values of the Western blot bands were measured with a Chemiscope Western Blot Imaging System (Clinx ChemiScope, Shanghai, China).
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