Fla 7000 scanner

Manufactured by Fujifilm

Sourced in Japan

The FLA-7000 is a high-performance laser scanner designed for imaging biopharmaceutical and molecular biology samples. It offers high-resolution scanning and versatile fluorescence detection capabilities.

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Lab products found in correlation

Phosphorimager screen, by Fujifilm (4 mentions) Imaging plate, by Fujifilm (2 mentions) Aida image analyzer, by Elysia raytest (2 mentions) Multi gauge software, by Fujifilm (2 mentions) Nylon membrane, by Merck Group (1 mentions) Multi gauge 3, by Fujifilm (1 mentions) Hybond n membrane, by Cytiva (1 mentions) Tri reagent, by Merck Group (1 mentions) Cryotome, by Leica (1 mentions) Tri reagent protocol, by Merck Group (1 mentions) Perfecthyb plus hybridization buffer, by Merck Group (1 mentions) Hybond n, by GE Healthcare (1 mentions) Decalabel dna labeling kit, by Thermo Fisher Scientific (1 mentions)

12 protocols using fla 7000 scanner

Whole-tissue and micro-autoradiography for atherosclerosis

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For whole-tissue autoradiography, the extracted aorta from 3 athero, control and WT mice was placed on an imaging plate (FujiFilm, Tokyo, Japan) for overnight exposure at −80°C. A digital readout of the imaging plate was obtained using a FLA-7000 scanner (FujiFilm, Tokyo, Japan), and images were analyzed with MultiGauge software (FujiFilm, Tokyo, Japan). Optical density corresponding to tracer uptake was quantified in PSL/mm². The entire aorta was excised vertically and subjected to ORO staining (see below).
For micro-autoradiography, the extracted aorta from 2 athero mice was embedded and frozen in optimal cutting temperature (OCT) compound. The frozen tissue samples were cut into 10 μm sections on a cryotome (Leica Microsystems, Bannockburn, IL). Autoradiographic images were obtained by placing tissue sections on an imaging plate (FujiFilm, Tokyo, Japan) for overnight exposure at −80°C. A digital readout was obtained using a FLA-7000 scanner (FujiFilm, Tokyo, Japan), and images were analyzed with MultiGauge software (FujiFilm, Tokyo, Japan). Adjacent sections were used for histologic studies. Digital autoradiographic images were visually co-registered with stained tissue sections and tracer uptake was quantified in regions of interest (ROI) that included the plaque and unaffected vessel wall in PSL/mm2.

Su H., Gorodny N., Gomez L.F., Gangadharmath U.B., Mu F., Chen G., Walsh J.C., Szardenings K., Berman D.S., Kolb H.C, & Tamarappoo B.K. (2014). Atherosclerotic plaque uptake of a novel integrin tracer 18F-Flotegatide in a mouse model of atherosclerosis. Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology, 21(3), 553-562.

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RNA Isolation and Small RNA Analysis

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For RNA isolation, phenol‐chloroform extraction procedure was carried out as described(Marin et al., 2010). Small RNA northern blot analysis was performed as described (Marin et al., 2010). RNA gel blots were hybridized with the miR390 probe together with U6 probe as a loading control. Non‐saturated signals were quantified on a Fuji FLA 7000 scanner.

Dastidar M.G., Scarpa A., Mägele I., Ruiz‐Duarte P., von Born P., Bald L., Jouannet V, & Maizel A. (2019). ARF5/MONOPTEROS directly regulates miR390 expression in the Arabidopsis thaliana primary root meristem. Plant Direct, 3(2), e00116.

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Yeast rDNA Copy Number Analysis

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Total yeast DNA was isolated as described previously [23 (link)]. Next, 10 μg of total DNA was digested with BamHI, an enzyme that does not cut within rDNA arrays. Then, PFGE was performed, followed by capillary transfer onto nylon membranes (Sigma-Aldrich) and Southern hybridization with the [α³²P] ATP-labeled RDN25-1 probe. Radioactive signals were registered using a FujiFilm FLA-7000 scanner.

Krol K., Jendrysek J., Debski J., Skoneczny M., Kurlandzka A., Kaminska J., Dadlez M, & Skoneczna A. (2017). Ribosomal DNA status inferred from DNA cloud assays and mass spectrometry identification of agarose-squeezed proteins interacting with chromatin (ASPIC-MS). Oncotarget, 8(15), 24988-25004.

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Stomach Autoradiography in Rats

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Immediately after microPET imaging, the rats were euthanized and the stomachs excised along the lesser curvature (Fig 1F). Excised stomachs were transferred to a chilled autoradiography cassette and stored for 12 h at -4°C. Screens were read using an FLA7000 scanner (Fujifilm, Tokyo, Japan). ROIs were selected on the greater curvature of the stomach where the blood supply was intact, and in the fundus where the blood supply was not intact because of partial devascularization of the stomach. The optical densities of autoradiographic signals were measured using Multi Gauge 3.2 software (Fujifilm, Tokyo, Japan). Autoradiographic images and ROIs were compared between the two groups.

Park S.Y., Kang W.J., Cho A., Chae J.R., Cho Y.L., Kim J.Y., Lee J.W, & Chung K.Y. (2015). 64Cu-ATSM Hypoxia Positron Emission Tomography for Detection of Conduit Ischemia in an Experimental Rat Esophagectomy Model. PLoS ONE, 10(6), e0131083.

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Differential Radial Capillary Action of Ligand Assays

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Differential Radial Capillary Action of Ligand Assays (DRaCALA) were performed essentially as described [58 (link)]. Briefly, in a 96-well round-bottom plate, purified proteins (WspR- positive control for GTP and c-di-GMP binding, Alg44- positive control for c-di-GMP binding, CbrR, CbrR_{KGRD(323-326)AAAA}, and CbrR_E334A) were diluted in buffer (10 mM Tris, pH 8.0, 5 mM MgCl₂, 100 mM NaCl). ³²P-labeled GTP (1:100) and ³²P-labeled c-di-GMP (1:2) were added to each protein and mixed via multi-channel pipet (to ensure similar mixing and timing. A pin tool was used to transfer solution to a flat nitrocellulose membrane by pressing firmly for 10–15 s. The membrane was allowed to dry completely and was imaged using a Fujifilm FLA-7000 scanner.

Cox C.A., Bogacz M., El Abbar F.M., Browning D.D., Hsueh B.Y., Waters C.M., Lee V.T, & Thompson S.A. (2021). The Campylobacter jejuni Response Regulator and Cyclic-Di-GMP Binding CbrR Is a Novel Regulator of Flagellar Motility. Microorganisms, 10(1), 86.

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Northern Blot Analysis of RNA in HEK293 Cells

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RNA was isolated from human Hek293 Flp-In T-REx-derived cell lines using TRI reagent (Sigma-Aldrich) according to manufacturer's instructions. Five micrograms of total RNA was separated by electrophoresis in a 1% formaldehyde-agarose gel prepared using TT buffer (30 mM tricine; 30 mM triethanolamine, pH = 8.0), followed by RNA immobilization on the Hybond N⁺ membrane (Amersham) by overnight capillary transfer in 20× SSC (3 M NaCl; 0.3 M sodium citrate). RNA was fixed by UV-crosslinking and the membrane was stained with methylene blue (0.03% solution in 0.3 M sodium acetate, pH = 5.3). Hybridizations were performed in PerfectHyb Plus hybridization buffer (Sigma-Aldrich). The blots were handled according to standard procedures and probed with 5′-labeled DNA oligonucleotides (sequences listed in Supplemental Table S1) at 42°C overnight. After each hybridization, membranes were washed twice for 30 min with 2× SSC, 0.1% SDS at 42°C, and eventually exposed to a PhosphorImager screen (FujiFilm), which was scanned using a FLA 7000 scanner (FujiFilm). Between successive hybridizations, probes were stripped off the membranes at 65°C using boiling 0.1% SDS.

Kobyłecki K., Drążkowska K., Kuliński T.M., Dziembowski A, & Tomecki R. (2018). Elimination of 01/A′–A0 pre-rRNA processing by-product in human cells involves cooperative action of two nuclear exosome-associated nucleases: RRP6 and DIS3. RNA, 24(12), 1677-1692.

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Quantifying Myocardial CP18 Uptake

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Hearts were embedded and frozen in optimal cutting temperature (OCT) compound, and frozen tissue was cut into 10 μm sections using a cryotome (Leica Microsystems, Bannockburn, IL). Autoradiographic images were obtained by placing the tissue sections on an imaging plate (FujiFilm, Tokyo, Japan) and exposed overnight at −80°C. CP18 uptake was quantified by measuring count densities using a FLA-7000 scanner (FujiFilm, Tokyo, Japan), image analysis software (Image J Software, NIH), and expressed as PSL/mm². Adjacent sections were used for histological staining studies.

Su H., Gorodny N., Gomez L.F., Gangadharmath U., Mu F., Chen G., Walsh J.C., Szardenings K., Kolb H.C, & Tamarappoo B. (2015). Noninvasive Molecular Imaging of Apoptosis in a Mouse Model of Anthracycline-Induced Cardiotoxicity. Circulation. Cardiovascular imaging, 8(2), e001952.

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Quantifying Protein Expression by In-Gel Fluorescence

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In-gel fluorescence blots were scanned with an FLA7000 scanner (Fuji). The band intensities were quantified using AIDA image analyzer (version 4.50, Raytest). Intensity of each band was calculated as a percentage of total pixel intensity of the lane. At least three biological replicates were performed for each experiment, means and standard deviations are presented in the figures. The intraclass correlation coefficient (ICC1) is given in figure legends.

Chapard C., Jones R., van Oepen T., Scheinost J.C, & Nasmyth K. (2019). Sister DNA Entrapment between Juxtaposed Smc Heads and Kleisin of the Cohesin Complex. Molecular Cell, 75(2), 224-237.e5.

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Southern Blot Quantification Protocol

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After hybridization, Southern blots were exposed to phosphorimager screens (Fuji) and scanned with an FLA7000 scanner (Fuji). The band intensities were quantified using AIDA image analyzer (version 4.50, Raytest). Intensity of each band was calculated as a percentage of total pixel intensity of the lane. At least three biological replicates were performed for each experiment, means and standard deviations are presented in the figures and figure legends.

Srinivasan M., Scheinost J.C., Petela N.J., Gligoris T.G., Wissler M., Ogushi S., Collier J.E., Voulgaris M., Kurze A., Chan K.L., Hu B., Costanzo V, & Nasmyth K.A. (2018). The Cohesin Ring Uses Its Hinge to Organize DNA Using Non-topological as well as Topological Mechanisms. Cell, 173(6), 1508-1519.e18.

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Southern Blot Phosphorimager Analysis

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After hybridization, Southern blots were exposed to phosphorimager screens (Fuji) and scanned with an FLA7000 scanner (Fuji). Scans shown in figures are representative of at least three independent experiments.

Chapard C., Jones R., van Oepen T., Scheinost J.C, & Nasmyth K. (2019). Sister DNA Entrapment between Juxtaposed Smc Heads and Kleisin of the Cohesin Complex. Molecular Cell, 75(2), 224-237.e5.

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