Acetylated α tubulin antibody

Manufactured by Merck Group

Sourced in United States

The Acetylated α-tubulin antibody is a lab equipment product used for the detection and analysis of acetylated alpha-tubulin, a post-translational modification of the alpha-tubulin protein. It is a useful tool for studying microtubule dynamics and organization in various cell types and biological processes.

Automatically generated - may contain errors

Lab products found in correlation

Primary antibodies specific, by Santa Cruz Biotechnology (1 mentions) Fluorescence microscopy, by Zeiss (1 mentions) Cy3 secondary antibodies, by Proteintech (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Viafect, by Promega (1 mentions) U2os osteosarcoma cells, by American Type Culture Collection (1 mentions) Vectashield containing dapi, by Vector Laboratories (1 mentions) Axioimager microscope, by Zeiss (1 mentions) Axiocam mrc camera, by Zeiss (1 mentions) Illustrator cs5, by Adobe (1 mentions) Lsm 780 nlo, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Pericentrin antibody, by Abcam (1 mentions) Pten antibody, by Cell Signaling Technology (1 mentions) γ tubulin antibody, by Merck Group (1 mentions) Gfp antibody, by Rockland Immunochemicals (1 mentions) Akt antibody, by Cell Signaling Technology (1 mentions) Tubastatin a, by Selleck Chemicals (1 mentions) Anti mouse igg hrp conjugate, by Promega (1 mentions)

5 protocols using acetylated α tubulin antibody

Osteoinduction Effects on Polycystin-2 and Primary Cilia

Check if the same lab product or an alternative is used in the 5 most similar protocols

hASCs were seeded on coverslips in 12-well plates to detect the effect of osteoinduction on polycystin-2 and the effect of PC2 knockdown on primary cilia formation by immunofluorescence. After 48 h, hASCs were fixed with 4% paraformal dehyde for 15 min and washed three times with PBS. Triton X-100 (0.5%, Sigma-Aldrich, United States ) was added to each well for 20 min at room temperature. After washing three times with PBS, hASCs were blocked with goat serum for 1 h. Primary antibodies specific for polycystin-2 (1:50, SANTA CRUZ, United States ) and acetylated α-tubulin antibody (diluted 1:100) (Sigma, Catalog #T7451) were added to the cells and then incubated overnight at 4°C. After washing three times with PBS Tween-20, fluorescent Cy3 secondary antibodies (1:50, Proteintech, United States ) were added and incubated for 1 h at 37 °C in the dark. The nucleus was then restained with DAPI (Sigma-Aldrich, United States ). Cells were subsequently viewed by fluorescence microscopy (ZEISS, Oberkochen, Germany).

Wang L., Lu Y., Cai G., Chen H., Li G., Liu L., Sun L., Guan Z., Sun W., Zhao C, & Wang H. (2022). Polycystin-2 mediates mechanical tension-induced osteogenic differentiation of human adipose-derived stem cells by activating transcriptional co-activator with PDZ-binding motif. Frontiers in Physiology, 13, 917510.

+ Open protocol

+ Expand

SPECC1L-GFP mutant expression in U2OS cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

SPECC1L-GFP expression constructs containing either wildtype or Thr397Pro, Gly1083Ser, Gln415Pro and ΔCHD mutations were transfected into U2OS osteosarcoma cells (ATCC, Manassas, Virginia, USA) using Viafect (Promega, Madison, Wisconsin, USA) or TransIT (Mirus Bio, Madison, Wisconsin, USA) according to manufacturer’s protocol. U2OS cells were grown in standard DMEM supplemented with 10% fetal bovine serum (FBS). Transfections and immunostaining were carried out on coverslips in 24-well plates. Cells were fixed in 4% paraformaldehyde (PFA) for 10 min, and blocked in phosphate buffered saline (PBS) with 1% goat serum and 0.1% Tween. Acetylated α-tubulin antibody (Sigma, St Louis, Missouri, USA) was used at 1:1000 dilution. After staining, coverslips were mounted in VectaShield containing DAPI (Vector Labs, Burlingame, California, USA).

Kruszka P., Li D., Harr M.H., Wilson N.R., Swarr D., McCormick E.M., Chiavacci R.M., Li M., Martinez A.F., Hart R.A., McDonald-McGinn D.M., Deardorff M.A., Falk M.J., Allanson J.E., Hudson C., Johnson J.P., Saadi I., Hakonarson H., Muenke M, & Zackai E.H. (2014). Mutations in SPECC1L, encoding sperm antigen with calponin homology and coiled-coil domains 1-like, are found in some cases of autosomal dominant Opitz G/BBB syndrome. Journal of medical genetics, 52(2), 104-110.

+ Open protocol

+ Expand

Visualizing Gene Expression in P. dumerilii

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gene expression patterns in P. dumerilii were assessed by whole-mount in situ hybridization, as previously described (31 (link)). After in situ hybridization, specimens were stained with DAPI (Invitrogen by Life Technologies) and immunohistochemically labeled with an acetylated α-tubulin antibody (Sigma-Aldrich) to visualize larval morphology and nervous system architecture (31 (link)). Developing P. dumerilii worms were subsequently imaged either as bright-field images with a Zeiss Axio Imager microscope and a Zeiss Axiocam MRc camera or as fluorescent images either by confocal reflection microscopy (31 (link)) with a Zeiss LD LCI Plan-Apochromat 25× 0.8 Oil/Glyc/Water DIC objective on a Zeiss LSM 780 NLO or by capturing the fluorescent spectra of the nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate precipitate (31 (link)) with an Olympus UPLSAPO 60× 1.3 silicon objective on a spinning disc confocal system Olympus IX83 (excitation, 633 nm; reflector, 785/762 nm) with an Andor iXon 888 Ultra camera. To illustrate the complete expression patterns, z sections of bright-field images were merged with the software Helicon Focus. We used Adobe Photoshop CS5.1 and Adobe Illustrator CS5 to create the final figures.

Handberg-Thorsager M., Gutierrez-Mazariegos J., Arold S.T., Kumar Nadendla E., Bertucci P.Y., Germain P., Tomançak P., Pierzchalski K., Jones J.W., Albalat R., Kane M.A., Bourguet W., Laudet V., Arendt D, & Schubert M. (2018). The ancestral retinoic acid receptor was a low-affinity sensor triggering neuronal differentiation. Science Advances, 4(2), eaao1261.

+ Open protocol

+ Expand

Antibody Dilutions for Western Blot and Immunofluorescence

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used to perform WB and immunofluorescence (IF) analysis. PTEN antibody (Cell Signaling Technology, cat# 9552S) dilutions for WB 1:2,000, for IF 1:300; acetylated α-tubulin antibody (Sigma-Aldrich, cat# T7451) 1:1,000 for IF; γ-tubulin antibody (Sigma-Aldrich, cat# T6557) 1:500 for IF; γ-tubulin antibody [TU-30] (Dyomics 647)(Abcam, ab#27076) 1:300 for IF; Pericentrin antibody (Abcam, cat# ab4448) 1:1,000 for IF; GFP antibody (Rockland, cat# 600-101-215) 1:500 for IF; RFP antibody (Abcam, cat# ab62341) 1:500 for IF; DVL 2 phospho-Serine-143 antibody (Abcam, cat# ab124933) 1:2,000 for WB; DVL2 antibody (Cell Signaling Technology, cat# 3224) 1:2,000 for WB; phospho-Akt (Ser473) (D9E) antibody (Cell Signaling Technology, cat #4060) 1:2,000 for WB; Akt antibody (Cell Signaling Technology cat#9272) 1:2,000 for WB; beta-actin antibody, clone AC-15 (Sigma, cat # A1978).

Shnitsar I., Bashkurov M., Masson G.R., Ogunjimi A.A., Mosessian S., Cabeza E.A., Hirsch C.L., Trcka D., Gish G., Jiao J., Wu H., Winklbauer R., Williams R.L., Pelletier L., Wrana J.L, & Barrios-Rodiles M. (2015). PTEN regulates cilia through Dishevelled. Nature Communications, 6, 8388.

+ Open protocol

+ Expand

HDAC Inhibition Assay and Visualization

Check if the same lab product or an alternative is used in the 5 most similar protocols

HDAC1 and HDAC6 were purchased from Abcam (AB101661 and AB42632), HDAC2 and HDAC3 were purchased from SignalChem (H84-30G and H85-30G). HDACs substrate Boc-Lys(acetyl)-AMC was purchased from Bachem. Pan-HDACs inhibitor SAHA, HDAC6 selective inhibitor tubastatin A and proteasome inhibitor carbobenzoxy-Leu-Leu-leucinal (MG132) were purchased from Selleckchem. The following antibodies were used for western blot: acetylated α-tubulin antibody (Sigma: T6793, 1:1000), β-actin antibody (Sigma: A1978, 1:5000), acetylated histone H4 antibody (Abcam: ab15823, 1:1000), anti-mouse IgG HRP conjugate (Promega: W402B, 1:10000) and anti-rabbit IgG HRP conjugate (Promega: W401B, 1:10000). The following antibodies were used for immunofluorescent staining: HDAC6 antibody (Santa Cruz: sc-11420, 1:200), ubiquitin antibody (Millipore: 05-1307, 1:500) and Alexa Fluor^® 647 goat anti-rabbit IgG (Invitrogen: A21245, 1:200).

Zhang Y., Yan J, & Yao T.P. (2017). Discovery of a fluorescent probe with HDAC6 selective inhibition. European journal of medicinal chemistry, 141, 596-602.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!