The extracted leaves paste of S. caseolaris from the ethanol solvent was dissolved into 1 mL methanol. The mixture was injected into the LC column at a flow rate of 0.2 mL/min at 30 °C. The system of LC-MS/MS was UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS from Thermo Fisher Scientific, Waltham, MA, USA. The ionisation mode on the MS system used was negative ionisation. The scan mode used was a full scan from 100–500 m/z. The UPLC column used was Accucore C18, 100 × 2.1 mm, 1.5 µm (Thermo Fisher Scientific, Waltham, MA, USA). The eluent used was H2O + 0.1% Formic acid (A) and acetonitrile + 0.1% formic acid (B). The results of the analysis were displayed using a mass chromatogram.
Uhplc vanquish tandem q exactive plus orbitrap hrms
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany
The UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS is a high-performance liquid chromatography-mass spectrometry system. It features a Vanquish UHPLC coupled with a Q Exactive Plus Orbitrap high-resolution mass spectrometer. The system provides high-resolution, accurate-mass detection for analytical applications.
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Lab products found in correlation
2 protocols using uhplc vanquish tandem q exactive plus orbitrap hrms
1
LC-MS/MS Analysis of S. caseolaris Extracts
2
Analytical Techniques for Compound Characterization
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The tools used in this research are cabinet dryer, blender, freezer, rotary evaporator, freeze dryer, 100 mL beaker, 250 and 500 mL Erlenmeyer flasks, stir bar, funnel, analytical balance, incubator, microplate reader, LC-MS/MS (Liquid Chromatography-Mass Spectrometry/Mass Spectrometry) UHPLC Vanquish Tandem Q Exactive Plus Orbitrap HRMS (ThermoScientific, Germany), computer set, software e.g., YASARA Structure, Discovery Studio Visualizer, Avogadro, and PyMol.
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