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10 protocols using 1 10 phenanthroline

1

Lanthanide-Based Polymer Synthesis Protocol

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The α-thenoyltrifluoroacetone (HTTA, 222.18, 98%) and 1,10-phenanthroline (Phen, 180.21, 99%) were purchased from Sinopharm Chemical Reagent Company (Shanghai, China). YbCl3·6H2O (387.50, 99.99%) and NdCl3·6H2O (358.74, 99.99%) were provided by Funing Rare Earth Industrial Company (Funing, China). Acrylic acid (AA, 72.06, 98%), methyl methacrylate (MMA, 100.12, 98%), ethylene glycol dimethylacrylate (EGDMA, 198.22, 98%), 2,2′-azobis(2-methylpropionitrile) (AIBN, 164.21, 99.5%), and N,N-dimethylformamide (DMF, 73.09, 99.5%) were obtained from Aladdin, Shanghai, China. All chemicals were used without further purification.
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2

Simultaneous Detection of Cd(II) and Hg(II) Ions

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L-arginine and standard reserve solutions of Cd2+ (892.9 μM), and Hg2+(498.5 μM) were obtained from Aladdin (Aladdin, Shanghai, China). Potassium chloride (KCl), sodium chloride (NaCl), magnesium chloride (MgCl2), calcium chloride (CaCl2), manganese chloride (MnCl2), zinc chloride (ZnCl2), aluminum chloride (AlCl3), nickel chloride (NiCl2.6H2O), sodium nitrate (NaNO3), sodium nitrite (NaNO2), sodium dihydrogen phosphate (NaH2PO4), sodium sulfate (Na2SO4), 1,10-phenanthroline, Rochelle salt, EDTA, sodium diphosphate, phytic acid, potassium iodide, acetate buffer, and trisodium citrate dihydrate were obtained from Sinopharm Chemical Reagent (Shanghai, China). All the reagents and solvents of analytical grades were used without further purification. All the experiments were carried out using deionized water.
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3

Synthesis and Characterization of Iron Oxide Nanoparticles

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All chemicals used in this study were of ACS-grade quality. High purity water (with resistivity > 18 MΩ·cm) from a Millipore Milli-Q purification system was used to prepare solutions. Iron nitrate nonahydrate (Fe(NO3)3·9H2O), hydroxylamine hydrochloride, sodium polyphosphate decahydrate, ammonium oxalate, dithionite, ammonium fluoride, 1,10-phenanthroline, acetic acid, sodium acetate, potassium hydroxide (KOH), and hydrochloric acid (HCl) were acquired from Sinopharm Chemical Reagent Co., Ltd. The pure sand particles were quartz with a particle size of <0.0450 mm was obtained from Tianjin Damao Chemical Reagent Co., Ltd.
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4

Production and Purification of A. niger HS-5 β-Glucanase

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Aspergillus niger HS-5 with high enzyme yield was screened by selective medium and compared its β-glucanase activity. The preparation and isolation of A. niger HS-5 β-glucanase was performed in house by using microbial fermentation. In brief, after fermentation, the cells in the fermented broth were removed by centrifugation to obtained crude enzyme solution, then ammonium sulfate salting out method was used to concentrate and Sephadex G-100 chromatography column was used for further purification.
Poria cocos were purchased from the Jiuzihe brand (Huanggang, China). Sulfuric acid, phenol, citric acid, sodium citrate, glucose, sodium dihydrogen phosphate, potassium ferricyanide, ferric chloride, trichloroacetic acid, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), anhydrous ethanol, hydrochloric acid, trometamol, pyrogallic acid, salicylic acid, 1,10-phenanthroline, potassium dihydrogen phosphate, dipotassium hydrogenphosphate, sodium chloride, ferrous sulfate, and hydrogen peroxide (30%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All reagents were of analytical grade.
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5

Preparation of Luminescent Reagents

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We directly dissolved L012 (Wako Pure Chemical Industries, Ltd., Osaka, Japan) and 1,10-phenanthroline (Sinopharm Co., Shanghai, China) in ultrapure water to prepare a 4 mM stock solution. Luminol (Sigma-Aldrich) and ABEI (TCI, Tokyo, Japan) were respectively dissolved in NaOH (pH 12) solution to prepare 4 mM stock solutions. Next, 2-imidazolidinetione, 2-methyl-3-thiosemicarbazide, and pyrogallic acid were purchased from Shanghai Acmec Biochemical Co., Ltd. (Shanghai, China). Catechin was purchased from Solarbio (Beijing, China). Dopamine was purchased from J&K Scientific (Beijing, China); DMPO was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan); and fluorescein and rhodamine B were purchased from Sangon Biotech (Shanghai, China). H2O2 was prepared fresh daily from 30% (vol/vol) H2O2 (Xinke Electrochemical Reagent Factory, Bengbu, China). All other reagents were obtained from Sinopharm Co. (Shanghai, China), of analytical grade. Ultrapure water was prepared by a Milli-Q system (Millipore, France) and used throughout.
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6

Terbium-based Luminescent Materials Synthesis

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Tb4O7 (99.99%), ammonia (25–28%), urea (99%) and cetyltrimethyl ammonium bromide (CTAB, 99%) were purchased from Sigma-Aldrich (Steinheim, Germany). 3-(triethoxysilyl)-Propyl isocyanate (TEPIC, 95%), 5-aminonicotinic acid (98%), 1,10-phenanthroline (phen, 99%), 2-thenoyltrifluoroacetone (TTA, 98%), tetraethoxysilane (TEOS, greater than 99%) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). All other chemicals were of analytical grade and used as received without further purification. The terbium perchlorate (Tb(ClO4)3 · nH2O) was prepared by dissolving Tb4O7 (99.99%) in HClO4 (1 mol l−1) and then evaporated and dried in vacuum.
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7

Penicillium digitatum Cultivation Protocol

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Penicillium digitatum DSM 62840 was purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). Glucose, ethylene diamine tetraacetic acid (EDTA), 12 H2O⋅Na2HPO4, 2 H2O⋅NaH2PO4, and 1,10-phenanthroline were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). R-(+)-Limonene and R-(+)-α-terpineol were obtained from Sigma Chemical Company (St. Louis, MO, United States). Hydrochloride (SKF-525A) and dithiothreitol (DTT) were purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). Malt extract was obtained from Beijing Shuangxuan Microbe Culture Medium Products Factory (Beijing, China). Yeast extract and peptone were purchased from Beijing Aoboxing Bio-Tech Co., Ltd. (Beijing, China). Phenylmethylsulfonyl fluoride (PMSF) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China).
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8

Synthesis of Diatomite-based Materials

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Diatomite (DIA, CP) was purchased from Ruijinte Chemical Co., Ltd. (Tianjin, China). Barium nitrate hexahydrate (Ce(NO3)2·6H2O) was procured from Guangfu Fine Chemical Research Institute (Tianjin). FeSO4, NH3·H2O, and NaOH were acquired from Damao Chemical Reagent Factory (Tianjin). Sodium chloride, methanol and sulfuric acid were obtained from Keanlong Bohua Pharmaceutical Chemical Co., Ltd. (Tianjin). Citric acid, sodium sulfate, ammonium ferrous sulfate, and hydroxylamine hydrochloride were acquired from Bodi Chemical Co., Ltd. (Tianjin, China). 1, 10-Phenanthroline was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All chemicals were of analytical grade.
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9

Analytical-Grade Materials for Research

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Analytical-grade materials and solvents were purchased from Sinopharm (Shanghai, China): ferrous sulfate (FeSO 4 Á7H 2 O), Lcysteine (C 3 H 7 NO 2 S), potassium dichromate (K 2 Cr 2 O 7 ), sulfuric acid (H 2 SO 4 ), nitric acid (HNO 3 ), sodium hydroxide (NaOH), 1,5diphenylcarbazide, 1,10-phenanthroline (Phen), sodium acetate (CH 3 COONa), hydroxylamine hydrochloride (NH 2 OHÁHCl), ethanol, ethylene glycol (EG), and acetone. All chemicals were used without further purification. Deionized water (>18 MXÁcm À1 ) was used for all experiments.
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10

Potassium Borohydride-Induced Oxidation Reactions

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Potassium borohydride (KBH4, 99%), ferrous sulfate heptahydrate (FeSO4·7H2O), sodium persulfate (Na2S2O8), sodium hydroxide (NaOH, 99%), rhodamine B (RhB), sulfuric acid (H2SO4, 95% to 98%), sodium acetate anhydrous (CH3COONa), ethanol, 1,10-phenanthroline (C12H8N2), tert-butyl alcohol (TBA), methanol (MeOH), 1,4-benzoquinone (BQ), acetone and ascorbic acid (H2A) were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd. All the solutions were prepared with deionized water before each run.
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