According to the standard protocols, paraffin-embedded duodenal tissue sections (4 μm) underwent an appropriate heat-induced antigen retrieval. Samples were blocked for 1 h, and incubated overnight at 4 °C with FITC-anti-rat-CD3 and PE-anti-rat-TCRγδ (Biolegend, San Diego, CA, USA). The next day samples were washed and stained with DAPI for 10 min, and imaged via fluorescent microscopy (Olympus, Japan).
Fitc anti rat cd3
Manufactured by BioLegend
Sourced in United States
FITC-anti-rat-CD3 is a monoclonal antibody conjugated with Fluorescein Isothiocyanate (FITC) that binds to the CD3 antigen expressed on rat T cells. It is designed for flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Pe anti rat tcrγδ, by BioLegend (1 mentions)
Fluorescent microscopy, by Olympus (1 mentions)
Lsrfortessa x 20, by BD (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Infinite m200 pro, by Tecan (1 mentions)
Pe antirat cd8, by BioLegend (1 mentions)
Chromatography grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
2 protocols using fitc anti rat cd3
1
Immunophenotyping of Duodenal T-cells
2
Isolation and Characterization of Rat PBMCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The isolation
kit for rat PBMCs and the rat peripheral blood lymphocyte separation
solution were purchased from Tianjin Haoyang Biological Products Technology
Co., Ltd. (Tianjin, China). IL-1β, IL-6, TNF-α, IL-2,
and IL-10 kits were purchased from Shanghai Xitang Biotechnology Co.,
Ltd. (Shanghai, China). Chromatography-grade acetonitrile, formic
acid, and methanol were provided from Thermo Fisher Scientific. The
following antibodies were used: FITC antirat CD3 (lot B279666, Biolegend,
San Diego, CA), APC antirat CD4 (lot B334589; Biolegend, San Diego,
CA), and PE antirat CD8 (lot B331642; Biolegend, San Diego, CA). BD
LSRFortessa X-20 was purchased from BD Biosciences. A Scientz-IID
Ultrasonic Cell Shredder was purchased from Ningbo Xinzhi Biological
Technology Co., Ltd. (Ningbo, China). A high-speed refrigerated centrifuge
was purchased from Shanghai Lishen Scientific Instrument Co., Ltd.
(Shanghai, China). A multifunctional microplate reader (TECAN Infinite
M200 Pro) was purchased from TECAN.
kit for rat PBMCs and the rat peripheral blood lymphocyte separation
solution were purchased from Tianjin Haoyang Biological Products Technology
Co., Ltd. (Tianjin, China). IL-1β, IL-6, TNF-α, IL-2,
and IL-10 kits were purchased from Shanghai Xitang Biotechnology Co.,
Ltd. (Shanghai, China). Chromatography-grade acetonitrile, formic
acid, and methanol were provided from Thermo Fisher Scientific. The
following antibodies were used: FITC antirat CD3 (lot B279666, Biolegend,
San Diego, CA), APC antirat CD4 (lot B334589; Biolegend, San Diego,
CA), and PE antirat CD8 (lot B331642; Biolegend, San Diego, CA). BD
LSRFortessa X-20 was purchased from BD Biosciences. A Scientz-IID
Ultrasonic Cell Shredder was purchased from Ningbo Xinzhi Biological
Technology Co., Ltd. (Ningbo, China). A high-speed refrigerated centrifuge
was purchased from Shanghai Lishen Scientific Instrument Co., Ltd.
(Shanghai, China). A multifunctional microplate reader (TECAN Infinite
M200 Pro) was purchased from TECAN.
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