Anti cd16 pe

Manufactured by Beckman Coulter

Sourced in United States, France

Anti-CD16-PE is a fluorescently-labeled antibody that binds to the CD16 cell surface receptor. It is used for the identification and quantification of CD16-positive cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

8 protocols using anti cd16 pe

E-cadherin Expression on PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The membrane expression of E-cad on PBMCs was investigated using flow cytometry. Cells were analyzed according to fluorescence intensity for CD3, CD14, CD16, and CD20, markers. MAb anti-CD3-FITC, anti-CD20-PC5, anti-CD16-PE, and anti-CD14-FITC were purchased from Beckman (Beckman coulter, Villepinte, France). Cell surface expression of E-cad was assayed using an anti-E-cad mAb-APC. Fluorescence intensity was measured using a Canto II cytofluorometer (Becton Dickinson, Biosciences, Le Pont de Claix, France), and the results were analyzed using a FlowJo software X.10.0.7.

Mezouar S., Omar Osman I., Melenotte C., Slimani C., Chartier C., Raoult D., Mege J.L, & Devaux C.A. (2019). High Concentrations of Serum Soluble E-Cadherin in Patients With Q Fever. Frontiers in Cellular and Infection Microbiology, 9, 219.

+ Open protocol

+ Expand

Leukocyte Dynamics in Acute Stroke

Check if the same lab product or an alternative is used in the 5 most similar protocols

The analyses of the first stroke cohort (Table 1) included evaluation of leukocytes, neutrophils, T cells, CD4 + T cells, CD8 + T cells, NK cells and B cells (anti-CD45 FITC, clone: B3821F4A; anti-CD56 PE, clone: N901/NKH-1; anti-CD16 PE, clone: 3G8, anti-CD19 ECD, clone: J3-119; anti-CD4 PE, clone: SFCI12T4D11; anti-CD8 PCD, clone: SFCI21Thy2D3; and anti-CD3_PC5, clone: UCHT1 [Beckman Coulter, Krefeld]) on days 0, 1, 2, 3, 4, 5, 7 post stroke. Cell analysis was performed on an FC500 (Beckmann Coulter, Krefeld) flow cytometer in the Department of Oncology of the University Medicine Greifswald. Cells were analysed on day of blood collection. Since all population could be clearly identified by the mentioned marker above no isotype controls were used.

Ruhnau J., Heuer C., Witt C., Ceesay S., Schulze J., Gross S., Waize M., Kromrey M.L., Kühn J.P., Langner S., Grunwald U., Bröker B.M., Petersmann A., Steveling A., Dressel A, & Vogelgesang A. (2023). Effects of body mass index on the immune response within the first days after major stroke in humans. Neurological Research and Practice, 5, 42.

+ Open protocol

+ Expand

Monocyte Subset Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monocyte subset analysis was performed using FACS Calibur flow cytometer (Becton Dickinson, United States) with monoclonal antibodies: anti-CD14-FITC, anti-CD14-PE, anti-CD11b-FITC, anti-CD18-PE, anti-CD54-FITC, anti-CD36-FITC, anti-CD16-PE, anti-CD206-PE (IO test, Beckman Coulter, France). 50 μl EDTA whole blood was stained with 5 μl of the relevant antibodies for 20 min at room temperature. In order to reduce Fc-receptor mediated binding of test antibodies, 2.5 μl Human Seroblock reagent (BIO-RAD, United States) were added for 10 min at room temperature. Red blood cells were lysed with 250 μl OptiLyse C Lysing Solution (Beckman Coulter, France) before analysis.

Buchheim J.I., Matzel S., Rykova M., Vassilieva G., Ponomarev S., Nichiporuk I., Hörl M., Moser D., Biere K., Feuerecker M., Schelling G., Thieme D., Kaufmann I., Thiel M, & Choukèr A. (2019). Stress Related Shift Toward Inflammaging in Cosmonauts After Long-Duration Space Flight. Frontiers in Physiology, 10, 85.

+ Open protocol

+ Expand

Quantifying Leukocyte Subsets by FACS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescence-activated cell sorting was used to measure cell counts for leukocytes, neutrophils, T cells, CD4+T cells, CD8+T cells, NK cells, and B cells (anti-CD45 FITC; anti-CD56 PE, anti-CD16 PE, anti-CD19 ECD, anti-CD3 PC5, IgG1 PC7, anti-CD4 PE, anti-CD8 PCD, anti-CD3 PC5 [Beckman Coulter]).

Vogelgesang A., Witt C., Heuer C., Schulze J., Gellrich J., von Sarnowski B., Langner S., Dressel A, & Ruhnau J. (2019). Clinical Improvement Following Stroke Promptly Reverses Post-stroke Cellular Immune Alterations. Frontiers in Neurology, 10, 414.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freshly isolated responders or the generated cells were analyzed with flow cytometry on days 0, 7, and 14. For the analysis of lymphocyte and monocyte lineage markers, the following conjugated antibodies (Abs) were used: anti-CD4-PerCP Cy5.5, anti-CD8-APC H7, anti-CD3-HV450, and anti-CD19-HV500 (all from BD Biosciences, San Jose, USA); and anti-CD16-PE, anti-CD56-PE, anti-CD14-PC7, and anti-CD45-APC AF700 (all from Beckman Coulter, Marseille, France). CD subsets were determined using the Lineage Cocktail 1 (lin 1; FITC-conjugated Abs against CD3, CD14, CD16, CD20, and CD56; all from BD Biosciences), anti-CD123-PECF594, anti-CD11c-PC7 (both from BD Biosciences), and anti-HLA DR-APC (Beckman Coulter). Tregs were identified using anti-CD127-PE, anti-CD4-PerCP Cy5.5 (BD Biosciences), anti-CD25 PC7 (Biolegend, San Diego, USA), and anti-FOXP3-AF647, clone 236A/E7 (BD Biosciences). The staining procedure, including fixation and permeabilization, was performed according to the manufacturer’s instructions (eBiosciences, San Diego, CA, USA). To prevent non-specific binding, mouse immunoglobulin G1 was added prior to incubation with the FOXP3 antibody. The data were acquired with a Navios flow cytometry instrument and analyzed using Kaluza software (Beckman Coulter, Brea, USA).

Watanabe M., Kumagai-Braesch M., Yao M., Thunberg S., Berglund D., Sellberg F., Jorns C., Enoksson S.L., Henriksson J., Lundgren T., Uhlin M., Berglund E, & Ericzon B.G. (2018). Ex Vivo Generation of Donor Antigen-Specific Immunomodulatory Cells: A Comparison Study of Anti-CD80/86 mAbs and CTLA4-lg Costimulatory Blockade. Cell Transplantation, 27(11), 1692-1704.

+ Open protocol

+ Expand

Flow Cytometric Analysis of Monocytes in COPD

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples from patients with COPD and from healthy blood donors were collected and incubated with 5 μL of anti-CD14-FITC, anti-CD16-PE and DRpc5-HLDR (Beckman Coulter, USA) antibodies for 10 min in the dark. Next, 2 mL of red cell lysis buffer (BD Biosciences, San Diego, CA, USA) were added for 10 min, and the tubes were centrifuged for 5 min at 500g and 20 °C. The supernatant was discarded, and the cell pellet was resuspended in 1× PBS. After further centrifugation for 5 min, the cell pellet was resuspended in 500 µL of 1% formalin in PBS. Measurements were performed with a FACSCalibur flow cytometer, and 200,000 events were obtained. T lymphocytes were excluded due to their lack of CD14, and natural killer cells and neutrophils were excluded due to their lack of HLDRA. The data were analyzed using Kalunza software (version 5, BD Biosciences). Results were expressed as individual values (%). Compensation was performed using antibodies with single-color fluorochromes for PE and FITC.

da Silva C.O., Gicquel T., Daniel Y., Bártholo T., Vène E., Loyer P., Pôrto L.C., Lagente V, & Victoni T. (2020). Alteration of immunophenotype of human macrophages and monocytes after exposure to cigarette smoke. Scientific Reports, 10, 12796.

+ Open protocol

+ Expand

Quantifying Lymphocyte Subsets in T1D

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peripheral blood mononuclear cells (PBMCs) from 80 patients with T1D were isolated. Forward and side scatter measurements were combined with CD45 to identify lymphocytes and exclude monocytes. The absolute subpopulations of lymphocyte numbers were calculated based on the total lymphocyte counts and the percentage of lymphocyte cell subpopulations, as identified by flow cytometry using EPICS XL (Beckman Coulter, Brea, CA, USA) and Divasoftware (BD Biosciences, Franklin Lakes, NJ, USA). The fraction of lymphocyte cell subsets was determined by multicolor fluorescence-activated cell sorter analysis using appropriate surface markers (Beckman Coulter): anti-CD3-FITC (Clone UCHT1), anti-CD4-PE-Cy7 (Clone 13B8.2), anti-CD8-APC-H7 (Clone B9.11), anti-CD16-PE (Clone 3GB), and anti-CD56-PE (Clone N901).

Zhang J.J., Wang J.Q., Xu X., Zhang L.D., Zhang C.P., Lu W.L., Gu W.Q., Dong Z.Y., Xiao Y, & Xia Z.W. (2022). Circulating circular RNA profiles associated with celiac disease seropositivity in children with type 1 diabetes. Frontiers in Pediatrics, 10, 960825.

+ Open protocol

+ Expand

Analyzing SARS-CoV-2 Receptor Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was used to study the membrane expression of ACE2 as well as specific biomarkers on PBMCs. Cells were analyzed according to fluorescence intensity using an anti-ACE2-monoclonal antibody (mAb) labeled with Alexa Fluor 488 (R&D Systems, Minneapolis, USA) and mAb anti-CD3-PC5, anti-CD20-PC7, anti-CD16-PE, and anti-CD14-APC purchased from Beckman (Beckman coulter, Villepinte, France). Fluorescence intensity was measured using a Canto II cytofluorometer (Becton Dickinson, Biosciences, Le Pont de Claix, France) and the results were analyzed using a BD FACSDiva software v.6. 1.3 (Becton Dickinson, New Jersey, USA).

Osman I.O., Melenotte C., Brouqui P., Million M., Lagier J.C., Parola P., Stein A., La Scola B., Meddeb L., Mege J.L., Raoult D, & Devaux C.A. (2021). Expression of ACE2, Soluble ACE2, Angiotensin I, Angiotensin II and Angiotensin-(1-7) Is Modulated in COVID-19 Patients. Frontiers in Immunology, 12, 625732.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!