Recombinant human SAA1 (hu rSAA1; 300-53), recombinant human CXCL8 (200-08M), recombinant human IL-1β (200-01B) and recombinant human TNF-α (300-01A) were purchased from PeproTech (Rocky Hill, NJ, USA). Recombinant murine SAA3 (mu rSAA3) was purchased from either MyBioSource (MBS1043052; San Diego, CA, USA) or Gentaur (CSB-EP361411Moe1; Kampenhout, Belgium). Lipopolysaccharide (LPS; 0111:B4) derived from E. coli and peptidoglycan (PGN; 77140) derived from Staphylococcus aureus were purchased from Sigma-Aldrich (St. Louis, MO, USA). TAK-242 (HY-11109) was purchased from MedChemExpress (Monmouth Junction, NJ, USA) and was solubilized in DMSO.
Tak 242 hy 11109
Manufactured by MedChemExpress
Sourced in United States
TAK-242 (HY-11109) is a small molecule compound manufactured by MedChemExpress. It is a selective inhibitor of Toll-like receptor 4 (TLR4) signaling. TAK-242 blocks the interaction between TLR4 and its intracellular adaptor molecules, thereby inhibiting TLR4-mediated inflammatory responses.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human tnf α 300 01a, by Thermo Fisher Scientific (2 mentions)
Jsh23, by Targetmol (1 mentions)
Ferrostatin 1 hy 100579, by MedChemExpress (1 mentions)
Mab208, by R&D Systems (1 mentions)
Recombinant human ifnγ 285 if, by R&D Systems (1 mentions)
Gapdh 2118, by Cell Signaling Technology (1 mentions)
Nf κb p65 sc 8008, by Santa Cruz Biotechnology (1 mentions)
Inos ab3523, by Abcam (1 mentions)
Traf6 ab33915, by Abcam (1 mentions)
4 protocols using tak 242 hy 11109
1
Recombinant Protein and Bacterial Component Treatments
2
Ferroptosis Inhibitors and Their Applications
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Ferrostatin-1 (HY-100579) and TAK-242 (HY-11109) were from MedChemExpress (Monmouth Junction, NJ, USA). Liproxstatin-1 (S81156) was from Yuanye Bio-Tech (Shanghai, China). Triacsin C (T139793) was from Aladdin (Shanghai, China). VX-765 (T6090), z-DEVD-FMK (T6005), GSK-872 (T4074), and JSH-23 (T1930) were from Targetmol (Wellesley Hills, MA, USA).
3
Recombinant HCV Proteins and Immune Modulation
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Recombinant HCV Core protein (MBS596029; Sequence ID BAM73001.1), NS3 protein (MBS596033; Sequence ID AIZ00641.1) and glycoprotein E2 (MBS434003; Sequence ID AAR22408.1) derived from genotype 1b were obtained from MyBiosource (San Diego, CA, USA). The lipopolysaccharide (LPS) content of the recombinant HCV proteins was as low as 0.02 ng/100 µg of core protein, 0.007 ng/100 µg of NS3 and 0.7 ng/100 µg of glycoprotein E2.
The maximal level of LPS reached in the highest tested concentrations of the viral proteins did not influence any of the activities reported here (data not shown). LPS (O111:B4) derived from E.coli and dsRNA (P1530) were obtained from Sigma-Aldrich (Overijse, Belgium). Mouse monoclonal IgG2a isotype control (MAB0031) and antibodies against human CCL2 (MAB679, BAF279), CXCL10 (BAF266) and CXCL8 (MAB208) were obtained from R&D Systems (Minneapolis, MN, USA). Recombinant human CCL3 (270-LD) and recombinant human IFN-γ (285-IF) were obtained from R&D Systems. Capture monoclonal anti-human CXCL10 (500-P93), recombinant human IL-1β (200-01B) and recombinant human TNF-α (300-01A) were obtained from Peprotech (London, UK). Mouse monoclonal anti-TLR2 IgG2a antibody (16-9922-82) was obtained from ThermoFischer Scientific (Merelbeke, Belgium). TAK-242 (HY-11109) was obtained from MedChemExpress (Monmouth Junction, NJ, USA).
The maximal level of LPS reached in the highest tested concentrations of the viral proteins did not influence any of the activities reported here (data not shown). LPS (O111:B4) derived from E.coli and dsRNA (P1530) were obtained from Sigma-Aldrich (Overijse, Belgium). Mouse monoclonal IgG2a isotype control (MAB0031) and antibodies against human CCL2 (MAB679, BAF279), CXCL10 (BAF266) and CXCL8 (MAB208) were obtained from R&D Systems (Minneapolis, MN, USA). Recombinant human CCL3 (270-LD) and recombinant human IFN-γ (285-IF) were obtained from R&D Systems. Capture monoclonal anti-human CXCL10 (500-P93), recombinant human IL-1β (200-01B) and recombinant human TNF-α (300-01A) were obtained from Peprotech (London, UK). Mouse monoclonal anti-TLR2 IgG2a antibody (16-9922-82) was obtained from ThermoFischer Scientific (Merelbeke, Belgium). TAK-242 (HY-11109) was obtained from MedChemExpress (Monmouth Junction, NJ, USA).
4
Quantification of Bioactive Compounds in PTH
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TAK-242 (HY-11109) was obtained from MedChemExpress (Monmouth Junction, NJ, USA). TLR4 (sc-293072), MyD88 (sc-74532), IκBα (sc-1643), and NF-κB p65 (sc-8008) primary antibodies were provided by Santa Cruz Biotechnology (Dallas, TX, USA). COX-2 (12282), GFAP (3670), p38 (8690), JNK (9252), ERK1/2 (9102), p-EEK1/2 (Thr202/Tyr204) (9101), and GAPDH (2118) primary antibodies were provided by Cell Signaling Technology (Danvers, MA, USA). iNOS (ab3523) and TNF receptor-associated factor 6 (TRAF6, ab33915) primary antibodies were provided by ABCAM (Cambridge, MA, USA). Each secondary antibody was provided by Thermo Fisher Scienti c (Rockland, IL, USA).
PTH capsules were purchased from Zhangzhou PTH Pharmaceutical Co., LTD. (Zhangzhou, China). In accordance with our reported UPLC-MS/MS method [22] , 21 characteristic compounds (taurine, malic acid, citric acid, cholic acid, hyodeoxycholic acid, ursodeoxycholic acid, chenodeoxycholic acid, taurocholic acid, taurochenodeoxycholic acid, tauroursodeoxycholic acid, glycodeoxycholic acid, glycocholic acid notoginsenoside R1, ginsenosides Rg1, Rb1, Re, Rf, Rd, Rg2, Rg3, Rh1, and muscone) in the PTH capsules were accurately quanti ed, and the total content of those compounds was 183.73 mg/g.
PTH capsules were purchased from Zhangzhou PTH Pharmaceutical Co., LTD. (Zhangzhou, China). In accordance with our reported UPLC-MS/MS method [22] , 21 characteristic compounds (taurine, malic acid, citric acid, cholic acid, hyodeoxycholic acid, ursodeoxycholic acid, chenodeoxycholic acid, taurocholic acid, taurochenodeoxycholic acid, tauroursodeoxycholic acid, glycodeoxycholic acid, glycocholic acid notoginsenoside R1, ginsenosides Rg1, Rb1, Re, Rf, Rd, Rg2, Rg3, Rh1, and muscone) in the PTH capsules were accurately quanti ed, and the total content of those compounds was 183.73 mg/g.
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