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Uvp gel documentation system

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The UVP gel documentation system is a laboratory instrument designed to capture and analyze images of DNA or protein gels illuminated by ultraviolet (UV) light. It provides a reliable and efficient way to document and analyze the results of electrophoresis experiments.

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Lab products found in correlation

Supersignal west femto chemiluminescent substrate kit, by Thermo Fisher Scientific (3 mentions) Supersignal west femto maximum sensitivity substrate kit, by Thermo Fisher Scientific (2 mentions) Electrophoresis system, by Bio-Rad (2 mentions) Anti gabab1 receptor antibody, by Santa Cruz Biotechnology (1 mentions) Anti nf κb p65 antibody, by Santa Cruz Biotechnology (1 mentions) Anti il 1β antibody, by Santa Cruz Biotechnology (1 mentions) Anti tnf α antibody, by Santa Cruz Biotechnology (1 mentions)

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Gel documentation system (54 citations)

6 protocols using uvp gel documentation system

1

Western Blot for Protein Quantification

Cited 1 time
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Western blot was used to determine the relative protein levels in cells and tissues with a protocol described previously with minor modification (Hua et al., 2016 (link)). Cells were harvested and lysed (150μl/106 cells) on ice. Protein concentration was measured using BCA assay kit. Thirty micrograms protein per sample was loaded onto the SDS-PAGE gel, fractioned using a Bio-Rad electrophoresis system, and then transferred from gel onto PVDF membranes. The protein containing membrane was blocked with 5% BSA for 60 minutes, incubated with primary antibodies at 4°C for 16 hours and then probed with a HRP-linked secondary antibody (#7074 for Rabbit IgG, #7076 for Mouse IgG, Cell Signaling Technology, Inc. Danvers, MA). The immunosignal was visualized with a SuperSignal West Femto Maximum Sensitivity Substrate Kit (#34096, Thermo Fisher Scientific, Waltham, MA). Images were captured and analyzed using a UVP gel documentation system (UVP, Upland, CA).
He C., Lv X., Huang C., Angeletti P.C., Hua G., Dong J., Zhou J., Wang Z., Ma B., Chen X., Lambert P.F., Rueda B.R., Davis J.S, & Wang C. (2019). A Human Papillomavirus-Independent Cervical Cancer Animal Model Reveals Unconventional Mechanisms of Cervical Carcinogenesis. Cell reports, 26(10), 2636-2650.e5.
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2

Quantitative Protein and mRNA Analysis

Cited 2 times
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Protein levels were determined by Western blot using a protocol established in our laboratory [23 (link)]. Immunosignals were visualized using a Thermo Scientific SuperSignal West Femto Chemiluminescent Substrate Kit (Thermo Fisher Scientific, Rockford, IL). The images were captured and analyzed with a UVP gel documentation system (UVP, LLC, Upland, CA). Expression of mRNA for BCL2, BRIC5, BAX, BAK1, and BAD was detected with quantitative PCR using a protocol previously established in our laboratory [27 (link)].
Lv X., He C., Huang C., Hua G., Chen X., Timm B.K., Maclin V.M., Haggerty A.A., Aust S.K., Golden D.M., Dave B.J., Tseng Y.A., Chen L., Wang H., Chen P., Klinkebiel D.L., Karpf A.R., Dong J., Drapkin R.I., Rueda B.R., Davis J.S, & Wang C. (2020). Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Development of High-Grade Cancer with Mesenchymal Lineage and Serous Features. Science bulletin, 65(15), 1281-1296.
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3

Western Blot Protein Quantification

Cited 1 time
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Protein levels in FTSECs, as well as in the xenograft tissues, were determined by Western Blot based on a protocol established in our laboratory19 , 72 (link). The immunosignal was detected using a Thermo Scientific SuperSignalWest Femto Chemiluminescent Substrate Kit (Thermo Fisher Scientific, Rockford, IL). The images were captured and analyzed with a UVP gel documentation system (UVP, LLC, Upland, CA).
Hua G., Lv X., He C., Remmenga S.W., Rodabough K.J., Dong J., Yang L., Lele S.M., Yang P., Zhou J., Karst A., Drapkin R.I., Davis J.S, & Wang C. (2015). YAP Induces High-Grade Serous Carcinoma in Fallopian Tube Secretory Epithelial Cells. Oncogene, 35(17), 2247-2265.
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4

Hippocampal Protein Expression Analysis

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In each group, five animals were sacrificed at T3, and the total protein was extracted from their hippocampal tissues. Protein levels of GABAB1 receptors, NF-κB, IL-1β and TNFα were analyzed using western blot assay. Briefly, total protein concentrations were determined using bicinchoninic acid assays (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), and western blot assays were performed as described above. Primary antibodies included the anti-GABAB1 receptor antibody (1:500, Santa Cruz Technology, Santa Cruz, CA), the anti-NF-κB p65 antibody (1:500, Santa Cruz Technology, Santa Cruz, CA), the anti-IL-1β antibody (1:500, Santa Cruz Technology, Santa Cruz, CA) and the anti-TNFα antibody (1:500, Santa Cruz Technology, Santa Cruz, CA), as well as the secondary horseradish peroxidase-labeled goat anti-rabbit immunoglobulin (1:4000, PTG Lab, Chicago, IL). The membrane was developed using enhanced chemiluminescence, exposed to X-ray film, and then photographed under UV light using a UVP gel documentation system (UVP, Upland, CA).
Li Z., Liu P., Zhang H., Zhao S., Jin Z., Li R., Guo Y, & Wang X. (2017). Role of GABAB receptors and p38MAPK/NF-κB pathway in paclitaxel-induced apoptosis of hippocampal neurons. Pharmaceutical Biology, 55(1), 2188-2195.
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5

Western Blot for Protein Quantification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blot was used to determine the relative protein levels in cells and tissues with a protocol described previously with minor modification (Hua et al., 2016 (link)). Cells were harvested and lysed (150μl/106 cells) on ice. Protein concentration was measured using BCA assay kit. Thirty micrograms protein per sample was loaded onto the SDS-PAGE gel, fractioned using a Bio-Rad electrophoresis system, and then transferred from gel onto PVDF membranes. The protein containing membrane was blocked with 5% BSA for 60 minutes, incubated with primary antibodies at 4°C for 16 hours and then probed with a HRP-linked secondary antibody (#7074 for Rabbit IgG, #7076 for Mouse IgG, Cell Signaling Technology, Inc. Danvers, MA). The immunosignal was visualized with a SuperSignal West Femto Maximum Sensitivity Substrate Kit (#34096, Thermo Fisher Scientific, Waltham, MA). Images were captured and analyzed using a UVP gel documentation system (UVP, Upland, CA).
He C., Lv X., Huang C., Angeletti P.C., Hua G., Dong J., Zhou J., Wang Z., Ma B., Chen X., Lambert P.F., Rueda B.R., Davis J.S, & Wang C. (2019). A Human Papillomavirus-Independent Cervical Cancer Animal Model Reveals Unconventional Mechanisms of Cervical Carcinogenesis. Cell reports, 26(10), 2636-2650.e5.
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6

Western Blot Protein Quantification

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein levels in FTSECs, as well as in the xenograft tissues, were determined by Western Blot based on a protocol established in our laboratory19 , 72 (link). The immunosignal was detected using a Thermo Scientific SuperSignalWest Femto Chemiluminescent Substrate Kit (Thermo Fisher Scientific, Rockford, IL). The images were captured and analyzed with a UVP gel documentation system (UVP, LLC, Upland, CA).
Hua G., Lv X., He C., Remmenga S.W., Rodabough K.J., Dong J., Yang L., Lele S.M., Yang P., Zhou J., Karst A., Drapkin R.I., Davis J.S, & Wang C. (2015). YAP Induces High-Grade Serous Carcinoma in Fallopian Tube Secretory Epithelial Cells. Oncogene, 35(17), 2247-2265.
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