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Novasyn tgr resin

Manufactured by Merck Group
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Sourced in Germany

NovaSyn TGR resin is a solid-phase resin used in organic synthesis. It is a crosslinked polystyrene-based resin containing a trityl (triphenylmethyl) group. The trityl group provides a temporary protecting group that can be selectively removed, allowing for further chemical modifications.

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Lab products found in correlation

N fluorenylmethoxycarbonyl fmoc protected amino acids, by Merck Group (2 mentions) Trifluoroacetic acid tfa, by Merck Group (2 mentions) Phenylboronic acid, by Merck Group (2 mentions) Sodium tetrachloropalladate, by Merck Group (2 mentions) Iodobenzene, by Merck Group (2 mentions) Deuterated dimethyl sulfoxide, by Merck Group (2 mentions) 4 iodotoluene, by Merck Group (2 mentions) N n dmethylformamide, by Merck Group (2 mentions) 4 iodobenzoic acid, by Merck Group (2 mentions) Biphenyl 4 carboxamide, by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Ascorbic acid, by Merck Group (2 mentions) Potassium carbonate, by Merck Group (2 mentions) Trifluoroacetic acid, by Merck Group (2 mentions) Pyridine, by Merck Group (2 mentions) Ethylene glycol, by Merck Group (2 mentions) Deuterated chloroform, by Merck Group (2 mentions) N n diisopropylethylamine, by Merck Group (1 mentions) N hydroxybenzotriazole, by Merck Group (1 mentions) 1 2 dimyristoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)

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TGR-resin (2 citations)

7 protocols using novasyn tgr resin

1

Peptide Synthesis and Lipid Preparation

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N-Fluorenylmethoxycarbonyl (Fmoc)-protected amino-acids, NovaSyn-TGR resin (loading 0.18 mM/g, 0.33 mM scale synthesis), and NovaSyn-TGRA resin (loading 0.18 mM/g, 0.33 mM scale synthesis) were purchased by Merck (Darmstadt, Germany); N,N-diisopropyl-ethylamine (DIEA), N,N-dimethylformamide (DMF, peptide synthesis grade), piperidine, dimethylformamide (DMF), N-hydroxybenzotriazole (HOBt), 2-(1-H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU), triisopropylsilane (TIS), cloroform, and trifluoroacetic acid (TFA), were purchased from Sigma-Aldrich (Munich, Germany). Peptide solution was prepared by dissolving lyophilized peptide in water. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was purchased from Avanti Polar Lipids (USA). Ultrapure Milli-Q water (resistivity > 18 MΩ·cm−1) was used for all experiments.
Rando C., Grasso G., Sarkar D., Sciacca M.F., Cucci L.M., Cosentino A., Forte G., Pannuzzo M., Satriano C., Bhunia A, & La Rosa C. (2023). GxxxG Motif Stabilize Ion-Channel like Pores through Cα―H···O Interaction in Aβ (1-40). International Journal of Molecular Sciences, 24(3), 2192.
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2

Solid-Phase Peptide Synthesis Protocol

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N-Fluorenylmethoxycarbonyl (Fmoc)-protected amino-acids, and NovaSyn-TGR resin (loading 0.18 mM/g, 0.33 mM scale synthesis)” resin were purchased by Merck (Darmstadt, Germany); N,N-diisopropyl-ethylamine (DIEA), N,N-dimethylformamide (DMF, peptide synthesis grade), piperidine, dimethylformammide (DMF), N-hydroxybenzotriazole (HOBt), 2-(1-H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU), triisopropylsilane (TIS), and trifluoroacetic acid (TFA), were purchased from Sigma-Aldrich (Munich, Germany). Peptide solution was prepared dissolving lyophilized peptide in water. Ultrapure Milli-Q water (resistivity > 18 MΩ·cm−1) was used for all experiments.
Magrì A, & La Mendola D. (2018). Copper Binding Features of Tropomyosin-Receptor-Kinase-A Fragment: Clue for Neurotrophic Factors and Metals Link. International Journal of Molecular Sciences, 19(8), 2374.
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3

Peptide-Peptoid Hybrid Synthesis

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Synthesis of PPS1 compound was done on NovaSyn TGR resin (EMD Millipore, MA). First three amino acids, Fmoc-Met-OH, Fmoc–D-Lys(Boc)-OH and Fmoc-Lys(Boc)-OH were loaded to the resin after Fmoc removal each time. Then 5-mer peptoid region containing Boc-Diaminobutane, 4-methoxybenzylamine, (R)-Methylbenzylamine, Piperonylamine and (R)-Methylbenzylamine was completed using microwave assisted peptoid synthesis protocol. At the end, beads were washed with DCM and cleaved off with TFA cleavage cocktail (Please refer to Supplementary Experimental Procedures for detailed procedures).
Desai T.J., Toombs J.E., Minna J.D., Brekken R.A, & Udugamasooriya D.G. (2016). Identification of lipid-phosphatidylserine (PS) as the target of unbiasedly selected cancer specific peptide-peptoid hybrid PPS1. Oncotarget, 7(21), 30678-30690.
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4

Synthesis of Gold and Palladium Nanoparticles

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Sodium tetrachloropalladate(II) (Na2PdCl4, 99.995%), gold(III) chloride hydrate (HAuCl4, ≥49% Au basis), sodium hydroxide (NaOH, ≥98%), sodium borohydride (NaBH4, ≥99%) cetyltrimethylammonium bromide (CTAB, ≥99%), ascorbic acid (≥99.0%), 1-hydroxybenzotriazole hydrate (HOBt, 99%), N,N’-diisopropylcarbodiimide (DIC, 99%), acetic anhydride (Ac2O, 99.5%), ethanol (≥99.5%), methanol (MeOH, ≥99.5%), 1-octanol (≥99%), dichloromethane (CH2Cl2, ≥99.9%), ethyl acetate (EtOAc, 99.8%), tetrahydrofuran (THF, ≥99.9%), ethylene glycol (EG, ≥99%), N,N-dmethylformamide (DMF, ≥99%), pyridine (≥99.9%), iodobenzene (98%), 4-iodotoluene (≥99%); 4-iodobenzoic acid (98%), biphenyl-4-carboxamide (96%), phenylboronic acid (PhB(OH)2, >98%), deuterated chloroform (CDCl3, 99.8%), deuterated dimethyl sulfoxide (DMSO-d6, 99.9%), trifluoroacetic acid (TFA, ≥99.0%), potassium carbonate (K2CO3, ≥99.0%) and magnesium sulfate (MgSO4, ≥99.5%) were all purchased from Sigma-Aldrich, and used as received without further purification except THF which was distilled to remove tutylated hydroxytoluene (BHT). NovaSyn TGR resin (0.25 mmol/g loading) was purchased from EMD Millipore. Sartorius ultrapure water (18.2 MΩ•cm) was used in all preparations.
Wei Z, & Matsui H. (2014). Rational strategy for shaped nanomaterial synthesis in reverse micelle reactors. Nature communications, 5, 3870.
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5

Synthesis of Peptoid-Lysine Conjugate

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PPS1D1 was synthesized on NovaSyn TGR resin (EMD Millipore, MA). First, Fmoc-Lys(Fmoc)-OH was coupled overnight as the central linker, and both Fmoc groups were removed simultaneously, allowing two copies of the sequence to be built on two amine groups of this central Lys. Three amino acids, Fmoc-Met-OH, Fmoc-D-Lys(Boc)-OH and Fmoc-Lys(Boc)-OH, were loaded onto the resin after Fmoc removal each time. The 5-mer peptoid region containing Boc-diaminobutane, 4-methoxybenzylamine, (R)-methylbenzylamine, piperonylamine and (R)-methylbenzylamine was synthesized using the microwave-assisted peptoid synthesis protocol. At the end of synthesis, the beads were washed 10 times with 2 ml DCM, the compound was cleaved with 2.5 ml of cleavage cocktail containing 95% TFA, 2.5% water, and 2.5% TIS on the shaker for 2 h, and the compound was purified using HPLC. Synthesis was confirmed using MALDI-TOF MS (Voyager DePro, AB Systems, MA).
Desai T.J, & Udugamasooriya D.G. (2017). A comprehensive lipid binding and activity validation of a cancer-specific peptide-peptoid hybrid PPS1. Biochemical and biophysical research communications, 486(2), 545-550.
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6

Automated Synthesis of C70PLY4 Peptide

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C70PLY4 was synthesized via a solid phase method using an automated peptide synthesizer (model PS-3 from Protein Technologies, Inc.), employing the fluorenylmethoxycarbonyl (Fmoc) group for α-amino group protection. The resin used was derived from a NovaSyn TGR resin with the modified Rink linker (Merck, Darmstadt, Germany). Tryptophan and lysine residues were protected with tert-butoxycarbonyl (tBoc), and arginine residues were protected with the 2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl (Pbf) group. The final de-blocking step was performed with a mixture of TFA/triisopropylsilane/water (94:3:3). The crude peptides were recovered by precipitation with diethyl ether as the non-solvent and then characterized using analytical reversed-phase HPLC. Mass spectrometry analyses were performed on an Agilent 1100 Series LC/MSD high-performance ion trap mass spectrometer to ensure that the target peptide was obtained. C70PLY4 was synthesized by Almac Sciences, UK.
Chang S.F., Chen C.N., Lin J.C., Wang H.E., Mori S., Li J.J., Yen C.K., Hsu C.Y., Fung C.P., Chong P.C., Leng C.H., Ding Y.J., Chang F.Y, & Siu L.K. (2020). Truncated Pneumolysin from Streptococcus pneumoniae as a TLR4-Antagonizing New Drug for Chronic Inflammatory Conditions. Cells, 9(5), 1183.
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7

Synthesis of Gold and Palladium Nanoparticles

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Sodium tetrachloropalladate(II) (Na2PdCl4, 99.995%), gold(III) chloride hydrate (HAuCl4, ≥49% Au basis), sodium hydroxide (NaOH, ≥98%), sodium borohydride (NaBH4, ≥99%) cetyltrimethylammonium bromide (CTAB, ≥99%), ascorbic acid (≥99.0%), 1-hydroxybenzotriazole hydrate (HOBt, 99%), N,N’-diisopropylcarbodiimide (DIC, 99%), acetic anhydride (Ac2O, 99.5%), ethanol (≥99.5%), methanol (MeOH, ≥99.5%), 1-octanol (≥99%), dichloromethane (CH2Cl2, ≥99.9%), ethyl acetate (EtOAc, 99.8%), tetrahydrofuran (THF, ≥99.9%), ethylene glycol (EG, ≥99%), N,N-dmethylformamide (DMF, ≥99%), pyridine (≥99.9%), iodobenzene (98%), 4-iodotoluene (≥99%); 4-iodobenzoic acid (98%), biphenyl-4-carboxamide (96%), phenylboronic acid (PhB(OH)2, >98%), deuterated chloroform (CDCl3, 99.8%), deuterated dimethyl sulfoxide (DMSO-d6, 99.9%), trifluoroacetic acid (TFA, ≥99.0%), potassium carbonate (K2CO3, ≥99.0%) and magnesium sulfate (MgSO4, ≥99.5%) were all purchased from Sigma-Aldrich, and used as received without further purification except THF which was distilled to remove tutylated hydroxytoluene (BHT). NovaSyn TGR resin (0.25 mmol/g loading) was purchased from EMD Millipore. Sartorius ultrapure water (18.2 MΩ•cm) was used in all preparations.
Wei Z, & Matsui H. (2014). Rational strategy for shaped nanomaterial synthesis in reverse micelle reactors. Nature communications, 5, 3870.
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