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2 protocols using ingeo biopolymer 2500hp

1

PLA Polymer Degradation Study

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In order to find a correlation between the polymer’s structure and susceptibility to degradation, different grades of PLA were chosen as the objects of this investigation, namely: Ingeo Biopolymer 2500HP, Ingeo Biopolymer 3100HP, Ingeo Biopolymer 3260HP, and Ingeo Biopolymer 4032D, all of which were supplied by Nature Works (Minnetonka, MN, USA). The chosen properties of the resins are presented in Table 1.
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2

Fabrication of Fluorescent Polymer Composites

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Polycaprolactone (PCL) (70,000–90,000 g mol−1), Polyvinylpyrrolidone (PVP) (360,000 g mol−1), dichloromethane (DCM) and dimethyl sulfoxide (DMSO) were obtained from Sigma–Aldrich (Switzerland), Polylactic acid (PLLA) (Ingeo™ Biopolymer 2500HP) from Natureworks, N,N- Dimethylformamide (DMF) from VWR Chemicals, Ethanol (without additive, ≥99.8%) and o-xylene from Fluka Analytical, chloroform (CHCl3, ≥99%) from Fischer Scientific and methanol (CH3OH, ≥99%) from Fluka. PtTFPP was from Frontier Scientific (USA). Calcein Green AM, cell growth medium, acetone, Na2SO3, KH2PO4, phosphate-buffered saline (PBS) and all the other reagents were from Sigma-Aldrich (Dun Laoghaire, Ireland). All materials were used without any further purification.
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