Rspo2
The RSPO2 is a laboratory equipment product manufactured by Merck Group. It is a device used for the detection and analysis of RNA-binding proteins. The core function of the RSPO2 is to facilitate the identification and study of these proteins, which play a crucial role in various cellular processes.
Lab products found in correlation
2 protocols using rspo2
Generation of RSPO2 expression constructs
Immunohistochemical Analysis of Lubricin, RSPOs, and LGRs
Lubricin, RSPOs and LGRs were immune stained using
the two-step immunohistochemistry procedure according
to the manufacturer’s protocol (Santa Cruz Biotechnology,
USA). In short, the tissue sections were treated with
rabbit polyclonal antibody for lubricin (Santa Cruz Immunohistochemistry
Lubricin, RSPOs and LGRs were immune stained using
the two-step immunohistochemistry procedure according
to the manufacturer’s protocol (Santa Cruz Biotechnology,
USA). In short, the tissue sections were treated with
rabbit polyclonal antibody for lubricin (Santa Cruz Biotechnology, USA), RSPO1 (Sigma-Aldrich, USA),
RSPO2 (Sigma-Aldrich, USA), LGR5 (Sigma-Aldrich,
USA), LGR6 (Abcam, England) and β-catenin (Santa
Cruz Biotechnology, USA) of 1:500 dilutions at 4˚C.
The slides were washed thrice with phosphate-buffered
saline (PBS, Wel Gene, Korea) and goat anti-rabbit
immunoglobulin G (IgG, Santa Cruz Biotechnology,
USA) was treated at room temperature for 30 minutes.
Western blot bands were developed for visualization with
3, 3′-diaminobenzidine as the chromogen. Each section
was photographed at ×10 magnification by a Zeiss Axio
Cam digital camera.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!