Metavue version v7

Manufactured by Molecular Devices

MetaVue version V7.7.8.0 is a software application developed by Molecular Devices for imaging and analysis of cellular and molecular samples. The core function of this software is to provide users with tools for image acquisition, processing, and analysis across a range of microscopy techniques.

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Lab products found in correlation

Ix70 microscope, by Olympus (3 mentions) Synergy 2 microplate reader, by Agilent Technologies (1 mentions) Oca 15 plus, by Dataphysics (1 mentions) Glass microscope slide, by Thermo Fisher Scientific (1 mentions) Synergy 2 plate reader, by Agilent Technologies (1 mentions) Du 520uv vis spectrophotometer, by Beckman Coulter (1 mentions)

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MetaVue (18 citations)

3 protocols using metavue version v7

Multimodal Imaging and Fluorometry

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Fluorescence microscopy images were acquired using an Olympus IX70 microscope and analyzed using the Metavue version V7.7.8.0 software package (Molecular Devices). Top-down scanning electron micrographs were acquired using a LEO-1550 VP field-emission SEM operating with an accelerating voltage of 2.00 kV. Samples were coated with a thin layer of gold using a Hummer Junior Sputtering (Technics) system operating at 10 mA under a vacuum pressure of 70 mTorr for 60 s prior to imaging. Fiber diameters were measured using the ImageJ version 1.49r software package. Solution fluorescence was measured using a Jobin Yvon FluoroMax-3 fluorometer with DataMax version 2.2 software. A Biotek Synergy 2 microplate reader with Gen5 software was used to measure the absorbance and fluorescence of bacterial cultures.

Kratochvil M.J., Yang T., Blackwell H.E, & Lynn D.M. (2017). Non-Woven Polymer Nanofiber Coatings that Inhibit Quorum Sensing in Staphylococcus aureus: Toward New Non-Bactericidal Approaches to Infection Control. ACS infectious diseases, 3(4), 271-280.

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Analysis of Surface Properties by RP-HPLC and Microscopy

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Reversed-phase
high-performance
liquid chromatography (RP-HPLC) was performed using a Shimadzu system
equipped with an SLC-10Avp controller, an LC-10AT pump, a FCV-ALvp
solvent mixer, and a SPC-10MAvp UV/vis diode array detector. An analytical
Phenomenex Gemini C18 column (5 μm, 4.6 × 250 mm², 110 Å) was used for analytical RP-HPLC work. A semipreparative
Phenomenex Gemini C18 column (5 μm, 10 × 250 mm², 110 Å) was used for preparative RP-HPLC work. Contact angle
measurements were made using a Dataphysics OCA 15 Plus instrument
with an automatic liquid dispenser at ambient temperature. Advancing
and receding contact angles were measured using 5 μL droplets
of deionized water (18 MΩ). Fluorescence microscopy images were
acquired using an Olympus IX70 microscope and analyzed using the Metavue
version V7.7.8.0 software package (Molecular Devices, LLC). Scanning
electron micrographs were acquired using a LEO 1530 scanning electron
microscope (SEM) at an accelerating voltage of 3 kV. SEM samples were
coated with a thin layer of gold using a gold sputterer operating
at 45 mA under a vacuum pressure of 50 mTorr for 40 s prior to imaging.
All statistical comparisons were made using Student’s t test.

Kratochvil M.J., Tal-Gan Y., Yang T., Blackwell H.E, & Lynn D.M. (2015). Nanoporous Superhydrophobic Coatings that Promote the Extended Release of Water-Labile Quorum Sensing Inhibitors and Enable Long-Term Modulation of Quorum Sensing in Staphylococcus aureus. ACS Biomaterials Science & Engineering, 1(10), 1039-1049.

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Synthesis and Characterization of PVDMA

Cited 1 time

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All chemicals were purchased from Sigma-Aldrich, unless indicated otherwise, and used without further purification. 2-Vinyl-4,4-dimethylazlactone (VDMA) was a gift from Dr. Steven M. Heilmann (3M Corporation, Minneapolis, MN). Poly(2-vinyl-4,4-dimethylazlactone) (PVDMA) was synthesized as described previously.^{66 (link)} Glass microscope slides were purchased from Fisher Scientific (Pittsburgh, PA). Dimethyl-2-aminobenzamidazole (DMABI)⁶⁰ and compounds E22^{67 (link)} and V-06-018³⁹ were synthesized as previously reported. Compressed air used to dry samples was filtered through a 0.2 µm membrane syringe filter. UV/vis measurements were made using a Beckman Coulter DU520 UV/vis spectrophotometer (Fullerton, CA). Fluorescence microscopy images were acquired using an Olympus IX70 microscope and analyzed using the Metavue version V7.7.8.0 software package (Molecular Devices). Absorbance measurements in biological assays were made using a Biotek Synergy 2 plate reader running Gen 5 software (version 1.05).

Kratochvil M.J., Welsh M.A., Manna U., Ortiz B.J., Blackwell H.E, & Lynn D.M. (2016). Slippery Liquid-Infused Porous Surfaces that Prevent Bacterial Surface Fouling and Inhibit Virulence Phenotypes in Surrounding Planktonic Cells. ACS infectious diseases, 2(7), 509-517.

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