Ski 2

Manufactured by Bio-Techne

Sourced in United Kingdom

SKI-II is a lab equipment product offered by Bio-Techne. It is a device used for the separation and purification of proteins and other biomolecules through a process called chromatography.

Automatically generated - may contain errors

Lab products found in correlation

Sphingosine 1 phosphate (s1p), by Avanti Polar Lipids (2 mentions) Tubulin antibody, by Santa Cruz Biotechnology (1 mentions) Anti akt1, by Santa Cruz Biotechnology (1 mentions) P c jun ser73, by Cell Signaling Technology (1 mentions) Sphk2, by Santa Cruz Biotechnology (1 mentions) Sphingosine 1 phosphate (s1p), by Cayman Chemical (1 mentions) C jun, by Santa Cruz Biotechnology (1 mentions) Sp600125, by Selleck Chemicals (1 mentions) Cisplatin, by Merck Group (1 mentions) 5 fluorouracil 5 fu, by Merck Group (1 mentions) Sphingosine 1 phosphate (s1p), by Bio-Techne (1 mentions) Clodronate and control liposomes, by Liposoma (1 mentions) Tamoxifen, by Merck Group (1 mentions) Corn oil, by Merck Group (1 mentions) Ccl22, by R&D Systems (1 mentions) Efluor 670, by Thermo Fisher Scientific (1 mentions) Rhosin, by Bio-Techne (1 mentions) Ccl21, by R&D Systems (1 mentions) Ccl19, by R&D Systems (1 mentions) U0126, by Bio-Techne (1 mentions)

7 protocols using ski 2

Ceramide and S1P Signaling Inhibitors

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ABC294640 was purchased from DC Chemicals (Shanghai, China). SKI-II (4-[[4-(4-Chlorophenyl)-2-thiazolyl]amino]phenol) was purchased from Tocris Bioscience (Ellisville, Mo). Cell permeable short-chain C6 ceramide was obtained from Avanti Polar Lipids, Inc. (Alabaster, AL). S1P was purchased from Cayman Chemical Co. (Ann Arbor, MI). 5-fluorouracil (5-FU) and cisplatin were purchased from Sigma (Shanghai, China). SP600125 and JNK inhibitor II (JNKi-II) were obtained from Selleck (Shanghai, China). Antibodies against phospho (p)-AKT (Ser 473), p-AKT (Thr 308), p-ribosomal protein S6 kinase 1 (S6K1) (Thr-389), p-JNK1/2 (Thr 183/Tyr 185) and p-c-Jun (Ser73) were purchased form Cell Signaling Tech (Denver MA). Anti-AKT1, SphK2, c-Jun and tubulin antibodies were obtained from Santa Cruz (Santa Cruz, CA).

Xun C., Chen M.B., Qi L., Tie-Ning Z., Peng X., Ning L., Zhi-Xiao C, & Li-Wei W. (2015). Targeting sphingosine kinase 2 (SphK2) by ABC294640 inhibits colorectal cancer cell growth in vitro and in vivo. Journal of Experimental & Clinical Cancer Research : CR, 34(1), 94.

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CHIKV Plasmid Cloning and Propagation

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CHIKV plasmids GFP-nsP1/2/3/4 and GFP-fused capsid were kindly provided by Dr Subhash G Vasudevan (Duke-NUS Graduate Medical School, Singapore). The pCMV6/Myc-DDK SK2 was purchased from OriGene (Rockville, MD, USA). SKI-II and S1P were purchased from Tocris and Cayman Chemical, respectively. ABC294640 was kindly provided by Dr Charles D Smith (MUSC, Charleston, SC, USA). CHIKV (clone 181/25) was propagated from a USAMRIID collection.

Reid S.P., Tritsch S.R., Kota K., Chiang C.Y., Dong L., Kenny T., Brueggemann E.E., Ward M.D., Cazares L.H, & Bavari S. (2015). Sphingosine kinase 2 is a chikungunya virus host factor co-localized with the viral replication complex. Emerging Microbes & Infections, 4(10), e61-.

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Lipid Biosynthesis and Modification Assay

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D-erythro-sphingosine (C18, 860490) and D-Galactosyl-β1-1’-N-Nervonoyl-D-erythro-sphingosine (C24:1, β-D-Galactosyl Ceramide, 110759), were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Glycoceramidase (E9030-100MUN), sphingomyelinase (S8633-25UN), Cy3-linked DBCO (777366-1 mg) and DAPI (D9542) were purchased from Millipore Sigma (Darmstadt, Germany). Recombinant mouse Asah2 (3558-AH) was purchased from R&D Systems (Minneapolis, MN, USA). The sphingosine kinase inhibitor SKI-II (CAS # 312636-16-1; Cat # 2097) was obtained from Tocris Bioscience (Bristol, United Kingdom) and tamoxifen (CAS # 10540-29-1, Cat # T5648-5G) and cornoil (CAS # 8001-30-7, Cat # C8267-500ML) from Millipore Sigma. Clodronate and control liposomes were purchased from Liposoma (CP-005-005, Amsterdam, Netherlands). Clickable ω-azido-sphingosine ((2S,3R,E)-2-amino-18-azidooctadec-4-ene-1,3-diol) for click chemistry^{42 (link)} staining was synthesized in-house by Julian Fink.

Lang J., Bohn P., Bhat H., Jastrow H., Walkenfort B., Cansiz F., Fink J., Bauer M., Olszewski D., Ramos-Nascimento A., Duhan V., Friedrich S.K., Becker K.A., Krawczyk A., Edwards M.J., Burchert A., Huber M., Friebus-Kardash J., Göthert J.R., Hardt C., Probst H.C., Schumacher F., Köhrer K., Kleuser B., Babiychuk E.B., Sodeik B., Seibel J., Greber U.F., Lang P.A., Gulbins E, & Lang K.S. (2020). Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease. Nature Communications, 11, 1338.

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Sphingosine-1-Phosphate Signaling Modulation

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S1P was from Avanti polar lipids (Alabaster, AL). Recombinant murine CCL2, CCL5, CCL19, CCL21, CCL22, CXCL10, CXCL12, IL-6, and TNFα were from R&D Systems (Minneapolis, MN). S1PR antagonists W146 (S1PR1), JTE013 (S1PR2), CYM 50358 hydrochloride (S1PR4), ERK inhibitor U0126, sphingosine kinase (Sphk) inhibitor Ski II, ROCK selective inhibitor Y-27632 and Rho selective inhibitor Rhosin were purchased from Tocris (Bristol, UK). Carboxyfluorescein diacetate succinimidyl ester (CFSE) and efluor670 were purchased from Molecular Probes (Eugene, OR). VCAM-1-Ig and ICAM-1-Ig were purchased from Sino Biological (Beijing, China). Anti-S1P mAb (clone LT1002) was kindly supplied by Dr. Roger A. Sabbadini (Lpath, Inc., San Diego, CA). All of the antibodies resources are indicated in Table S1.

Xiong Y., Piao W., Brinkman C.C., Li L., Kulinski J.M., Olivera A., Cartier A., Hla T., Hippen K.L., Blazar B.R., Schwab S.R, & Bromberg J.S. (2019). CD4 T cell sphingosine 1-phosphate receptor (S1PR)1 and S1PR4 and endothelial S1PR2 regulate afferent lymphatic migration. Science immunology, 4(33), eaav1263.

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Icaritin Protocol for HCC Studies

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Icaritin (purity 98%) was purchased from Shenogen Pharma Group (Beijing, China). Icaritin was dissolved in dimethyl sulfoxide (DMSO) for in vitro studies. The final concentration of DMSO in the culture media was maintained at 0.1%, which showed no cytotoxicity to HCC cells. For in vivo studies, icaritin was dissolved in the SX-1292 oral vehicle [1% sodium carboxymethyl cellulose, 0.5% sodium lauryl sulfate (SLS), and 0.05% antifoam; Eli Lilly, Shanghai, China] and administered by gastric lavage. D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP), FTY720 and SP600125 were obtained from Sigma (St. Louis, MO); SKI-II (4-[[4-(4-Chlorophenyl)-2-thiazolyl]amino]phenol) and sorafenib were purchased from Tocris Bioscience (Ellisville, Mo). Sphingosine 1-phosphate (S1P) and C6 ceramide were obtained from Avanti Polar Lipids, Inc. (Alabaster, AL). All antibodies utilized in this study were purchased from Cell Signaling Technology (Beverly, MA).

Lu P.H., Chen M.B., Liu Y.Y., Wu M.H., Li W.T., Wei M.X., Liu C.Y, & Qin S.K. (2017). Identification of sphingosine kinase 1 (SphK1) as a primary target of icaritin in hepatocellular carcinoma cells. Oncotarget, 8(14), 22800-22810.

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Apoptosis and Necrosis Inhibitors

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The following inhibitors were used in the study: 20 μM Z-VAD-FMK (Invivogen), 1 μM GSK’872 (Selleck Chemicals), 5 μM Necrosulfonamide (Calbiochem), 50 µM blebbistatin (Sigma-Aldrich), 30 µM Y-27632 (StemCell), 15 µM thiazovivin (StemCell), 50 µM ML-141 (Sigma-Aldrich), 30 µM ML-7 (Tocris), 200 µM NSC-23766 (Tocris), 20 µM JTE-013 (Sigma-Aldrich), and 30 µM SKI-II (Tocris). All inhibitors were dissolved in DMSO and diluted to 10× concentration in OptiMEM, then added directly to the cells to achieve 1× concentration at least 1 h before imaging.

Shkarina K., Hasel de Carvalho E., Santos J.C., Ramos S., Leptin M, & Broz P. (2022). Optogenetic activators of apoptosis, necroptosis, and pyroptosis. The Journal of Cell Biology, 221(6), e202109038.

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Assessing A549 Cell Proliferation

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) were stained by means of carboxyfluorescein diacetate succinimidyl ester (CFSE; 1µM; proliferation Dye, eBioscience Inc., San Diego, CA; Cat#65-0850-85) to perform proliferation assay. CFSE flow cytometry data was analysed by means of ModFit4.0 software (BD Pharmingen). A549 cells were treated for 48 hours with sphingosine-1-phosphate (S1P 10 nM; Sigma-Aldrich, Merck Life Science S.r.l., Milan, Italy; Cat#S9666) or unmethylated Cytosine phosphate Guanosine-oligodeoxynucleotides, CpG-ODN (CpG 1 μg/ml; Vincibiochem, Milan, Italy) in presence of specific pharmacological inhibitors, such as TY52156, a S1PR3 receptor antagonist (S1PR3a 10 µM; Tocris Bioscience, Ellisville, MO; Cat#5328), anti-Spinster homolog 2 antibody (αSPNS2 10 ng/ml; Abcam, Milan, Italy; Cat#ab82629), SKI II, a selective inhibitor of sphingosine kinases (SKI II 10 µM; Tocris Bioscience, Ellisville, MO; Cat#2097).

Terlizzi M., Colarusso C., Ferraro G., Monti M.C., Rosa I., Somma P., Salvi R., Pinto A, & Sorrentino R. (2021). Intracellular Sphingosine-1-Phosphate Receptor 3 Contributes to Lung Tumor Cell Proliferation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 55(5).

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