Pires2 egfp expressing plasmid

Manufactured by Takara Bio

Sourced in United States

The PIRES2-EGFP expressing plasmid is a laboratory tool designed for the expression of enhanced green fluorescent protein (EGFP) in cells. This plasmid contains the PIRES2 promoter, which drives the expression of the EGFP gene. The PIRES2-EGFP plasmid can be used for various applications, such as monitoring gene expression or as a reporter for transfection efficiency.

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PIRES2-EGFP (75 citations) PIRES2-EGFP plasmid (11 citations)

3 protocols using pires2 egfp expressing plasmid

SARS-CoV-2 S Variant Plasmid Cloning

Cited 2 times

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The plasmids encoding the different SARS-CoV-2 S variants (D614G, Delta, BA.1 and BA.2) were previously described [15 (link),16 (link),27 (link),32 (link)]. The pIRES2-EGFP expressing plasmid was purchased from Clontech, Mountain View, CA, USA (Cat# 6029-1).

Marchitto L., Chatterjee D., Ding S., Gendron-Lepage G., Tauzin A., Boutin M., Benlarbi M., Medjahed H., Sylla M., Lanctôt H., Durand M., Finzi A, & Tremblay C. (2023). Humoral Responses Elicited by SARS-CoV-2 mRNA Vaccine in People Living with HIV. Viruses, 15(10), 2004.

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Generating SARS-CoV-2 Spike Variant Plasmids

Cited 1 time

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The plasmids encoding the SARS-CoV-2 D614G, BA.1, BA.4/5, and BQ.1.1 Spike variants were previously described [10 (link),20 (link),33 (link)]. The pNL4.3 R-E-Luc plasmid was obtained from the NIH AIDS Reagent Program (cat# 3418). The pIRES2-EGFP-expressing plasmid was purchased from Clontech, Mountain View, CA, USA (cat# 6029-1). The plasmids encoding XBB, XBB.1.5, CH.1.1, and FD.1.1 Spikes were generated by overlapping PCR. Sequences were verified by Sanger sequencing.

Tauzin A., Beaudoin-Bussières G., Benlarbi M., Nayrac M., Bo Y., Gendron-Lepage G., Medjahed H., Perreault J., Gokool L., Arlotto P., Morrisseau C., Tremblay C., Kaufmann D.E., Martel-Laferrière V., Levade I., Côté M., Bazin R, & Finzi A. (2023). Humoral Responses Elicited after a Fifth Dose of SARS-CoV-2 mRNA Bivalent Vaccine. Viruses, 15(9), 1926.

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SARS-CoV-2 Spike Variant Plasmids

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The plasmids encoding the SARS-CoV-2 D614G and BQ.1.1 Spike variants were previously described [4 (link)]. The pNL4.3 R-E-Luc plasmid was obtained from the NIH AIDS Reagent Program (Cat# 3418). The pIRES2-EGFP expressing plasmid was purchased from Clontech, Mountain View, CA, USA (Cat# 6029-1).

Tauzin A., Benlarbi M., Medjahed H., Grégoire Y., Perreault J., Gendron-Lepage G., Gokool L., Morrisseau C., Arlotto P., Tremblay C., Kaufmann D.E., Martel-Laferrière V., Levade I., Côté M., De Serres G., Bazin R, & Finzi A. (2023). Humoral Responses against BQ.1.1 Elicited after Breakthrough Infection and SARS-CoV-2 mRNA Vaccination. Vaccines, 11(2), 242.

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