LOX-1, CRP, IL-6, CXCL2, and oxLDL concentrations in mouse and human BALF were determined using ELISA, per manufacturer’s instructions (R&D Systems; sLOX-1, CRP, IL-6, and CXCL2) (CUSABIO; murine oxLDL) (Mercodia; human oxLDL). LDH was measured using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) per manufacturer’s instructions. Additional cytokines were measured using a mouse magnetic Luminex assay (R&D Systems) and read on a LiquiChip 200 workstation (Qiagen).
Liquichip 200 workstation
Manufactured by Qiagen
Sourced in United States, Germany
The LiquiChip 200 workstation is a laboratory instrument designed for automated liquid handling and sample processing. It provides precise and consistent pipetting, mixing, and incubation capabilities for various applications in life science research and diagnostics.
Automatically generated - may contain errors
Lab products found in correlation
Mouse magnetic luminex assay, by R&D Systems (1 mentions)
Cytotox 96 non radioactive cytotoxicity assay, by Promega (1 mentions)
Liquichip analyser software, by Qiagen (1 mentions)
Bio plex pro mouse cytokine th17 panel a 6 plex kit, by Bio-Rad (1 mentions)
Serum gel z tubes, by Sarstedt (1 mentions)
Bio plex manager software, by Bio-Rad (1 mentions)
3 protocols using liquichip 200 workstation
1
Comprehensive Biomarker Profiling in Respiratory Disease
2
Cytokine Quantification in Supernatants
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Supernatants were kept at −20 °C and transported to Leiden, the Netherlands, where cytokine responses were quantified using Luminex cytokine kits (Biosource, Camarillo, CA, USA) and a Liquichip 200 Workstation (Qiagen, Venlo, the Netherlands) equipped with Liquichip analyser software (Qiagen, Venlo, the Netherlands). Cytokine levels below the assay’s detectable range were replaced by half the detection limit. The detection limits were 2.26 pg/mL for TNF, 0.68 pg/mL for IFN-γ, 0.16 pg/mL for IL-5, 0.44 pg/mL for IL-13, 0.60 pg/mL for IL-17, and 0.78 pg/mL for IL-10.
3
Multiplex Cytokine Analysis in Mouse Serum
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Blood was collected from sacrificed mice via inferior vena cava using a 22 G needle and transferred to serum‐gel Z tubes (Sarstedt, Germany), allowed to clot for 30 min at room temperature and centrifuged at 10 000 g for 5 min. The serum was collected and stored frozen until use. To detect interleukin‐1 beta (Il‐1β), interleukin‐6 (Il‐6), and tumour necrosis factor‐alpha (Tnf‐α) simultaneously, Bio‐Plex Pro™ mouse cytokine Th17 panel A 6‐Plex kit (Bio‐Rad, Germany) was used according to the manufacturer's instructions. Samples were diluted at 1:2, and the fluorescence measurement of the beads was performed with the Qiagen LiquiChip 200 workstation (Hilden, Germany). Cytokine concentrations were calculated using Bio‐Plex Manager software (Bio‐Rad, Hercules, CA, USA).
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