MCF7/Par and T47D/Par cells were lysed using RIP assay buffer (Millipore, Billerica, MA, USA), and then incubated with magnetic beads coated with anti-Ago2. Normal mouse IgG was served as a negative control. Finally, purified RNA was subjected to qRT-PCR analysis to determine the presence of the binding targets.
Rip assay buffer
Manufactured by Merck Group
Sourced in United States
The RIP assay buffer is a laboratory reagent used for the detection and quantification of protein-RNA interactions in cell and tissue samples. It serves as a key component in the RNA Immunoprecipitation (RIP) assay, a widely used technique to study the association between RNA-binding proteins and their target RNAs. The buffer supports the efficient lysis of cells, the solubilization of protein-RNA complexes, and the preservation of these interactions for downstream analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rip assay buffer
1
Identification of Ago2-Bound Transcripts
2
Investigating miR-328-3p and FOXO4 Interaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Magna RNA immunoprecipitation kit (Millipore, Billerica, MA, USA) was applied to investigate the interaction between miR-328-3p and FOXO4 in accordance with the protocols of the manufacturer. HUVECs transfected with miR-NC or miR-328-3p were lysed using RIP assay buffer (Millipore), then cell extracts were coimmunoprecipitated with anti-Ago2, and normal rabbit IgG was used as a negative control. Finally, the enrichment of FOXO4 was extracted and analyzed.
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