L929 cell line derived from murine fibroblasts (ATCC CCL-1™) and U-2 OS cell line derived from human osteosarcoma (ATCC HTB-96™) were maintained in MEM (Minimal Essential Medium, Sigma, M0446) and DMEM (Dulbecco’s Modified Eagle’s Medium, Sigma, D0819), respectively with 10% FBS at standard conditions of 37 °C, 5% CO2 and 100% relative humidity. Cells were used from the 3rd passage after thawing and only till the 20th passage. Cells were passaged regularly using trypsin–EDTA solution (Sigma, T4049) which was stopped by addition of the medium with FBS with no subsequent centrifugation.
Htb 96
Manufactured by Merck Group
The HTB-96 is a high-throughput microplate reader designed for a variety of life science applications. It features a compact and efficient design, allowing for rapid and accurate data acquisition. The core function of the HTB-96 is to measure absorbance, fluorescence, and luminescence in 96-well microplates, supporting a wide range of assays and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta solution, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by InvivoGen (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
X tremegene hp transfection reagent, by Roche (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
L glutamine, by InvivoGen (1 mentions)
Geneticin, by InvivoGen (1 mentions)
Fetal bovine serum (fbs), by InvivoGen (1 mentions)
Crl 1690, by American Type Culture Collection (1 mentions)
Mem non essential amino acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Gentamicin, by Merck Group (1 mentions)
F 12 ham, by Merck Group (1 mentions)
4 protocols using htb 96
1
Culturing Murine and Human Cell Lines
2
Generation of Stable GFP-AURKA Cell Lines
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U2OS cells free from mycoplasma were purchased from American Type Culture Collection (ATCC, HTB-96) and were grown in Dulbecco's modified Eagle's medium (DMEM, Sigma-Aldrich) supplemented with 10% fetal bovine serum (Life Technologies, Thermo Fisher Scientific), 1% L-glutamine (Life Technologies, Thermo Fisher Scientific) and 1% penicillinstreptomycin (Life Technologies, Thermo Fisher Scientific). Cells were cultivated at 37°C and 5% CO2. The generation of GFP-AURKA and GFP-AURKA-mCherry stable cell lines were generated by transfecting U2OS cells with X-tremeGENE HP transfection reagent (Roche), following the manufacturer's indications. Stable clones were selected in DMEM supplemented with 10% fetal bovine serum, 1% L-glutamine, 1% penicillin-streptomycin and 500 μg/ml Geneticin (Invivogen). Stable and transient transfections were performed with X-tremeGENE HP transfection reagent (Roche) according to the manufacturer's instructions.
3
Culturing Glioblastoma, Astrocyte, and Osteosarcoma Cells
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The T98G glioblastoma multiforme cell line (ATCC CRL-1690) was obtained from ATCC and cultured based on the specifications provided by ATCC. The human astrocyte cell line (ScienCell 1800) was purchased from ScienCell and was similarly cultured based upon the directions provided by ScienCell. GM00637 normal human fibroblasts, SV-40 transformed (Coriell) and U2OS human bone osteosarcoma epithelial cells (ATCC HTB-96) were cultured in DMEM (Sigma D5796) containing 10% FBS (Gemini 100-106) and 1% penicillin/streptomycin (Gibco 15070063).
4
Culturing U2OS Osteosarcoma Cells
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The U2OS human bone osteosarcoma cell line (DSMZ, Braunschweig Germany) was obtained from ATCC (cat. HTB-96) and grown in Dulbecco's modified Eagle's medium nutrient mixture F-12 HAM (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (Sigma-Aldrich), MEM nonessential amino acids (Sigma-Aldrich), and gentamicin (Sigma-Aldrich) at 37 °C in a humidified atmosphere supplemented with 5% CO 2 .
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