Igrov 1

Manufactured by Merck Group

Sourced in United States

IGROV-1 is a laboratory cell line. It is an ovarian cancer cell line derived from a human ovarian carcinoma.

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7 protocols using igrov 1

Ovarian Cancer Cell Line Characterization

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The ovarian cancer cell lines (OCCLs) A2780, IGROV-1, OVCAR-8, and SK-OV-3 were used in this work. OVCAR-8 and SK-OV-3 were acquired from the American Type Culture Collection (ATCC), A2780 from the European Collection of Authenticated Cell Cultures (ECACC), and IGROV-1 from Merck Millipore, Burlington, MA, USA. IGROV-1 and A2780 were cultured in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco). SK-OV-3 and OVCAR-8 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were incubated at 37 °C in a 5% CO₂ atmosphere. The presence of mycoplasma was routinely checked with the MycoAlert kit (Lonza, Basel, Switzerland), and only mycoplasma-free cells were used in the experiments.

Ovejero-Sánchez M., Rubio-Heras J., Vicente de la Peña M.D., San-Segundo L., Pérez-Losada J., González-Sarmiento R, & Herrero A.B. (2022). Chloroquine-Induced DNA Damage Synergizes with Nonhomologous End Joining Inhibition to Cause Ovarian Cancer Cell Cytotoxicity. International Journal of Molecular Sciences, 23(14), 7518.

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Cultivation and Characterization of Ovarian and Multiple Myeloma Cell Lines

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The ovarian cancer cell lines (OCCLs) IGROV-1, OVCAR-8, SK-OV-3 and A2780 were acquired from American Type Culture Collection (ATCC) (OVCAR-8, SK-OV-3), European Collection of Authenticated Cell Cultures (ECACC) (A2780) and Merck Millipore (IGROV-1). Multiple myeloma cell line JJN3-HR was previously constructed in our group [12] (link). IGROV-1, A2780 and JJN3-HR were cultured in RPMI 1640 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Gibco). SK-OV-3 and OVCAR-8 were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were incubated at 37°C in a 5% CO₂ atmosphere. The presence of mycoplasma was routinely checked with MycoAlert kit (Lonza) and only mycoplasma-free cells were used in the experiments.

Ovejero-Sánchez M., González-Sarmiento R, & Herrero A.B. (2021). Synergistic effect of Chloroquine and Panobinostat in ovarian cancer through induction of DNA damage and inhibition of DNA repair. Neoplasia (New York, N.Y.), 23(5), 515-528.

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Ovarian Cancer Cell Line Cultivation

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Ovarian cancer cell lines (OCCLs) IGROV-1, OVCAR-8, SK-OV-3, and A2780 were used in this work. OVCAR-8 and SK-OV-3 were obtained from the American Type Culture Collection (ATCC), A2780 from the European Collection of Authenticated Cell Cultures (ECACC), and IGROV-1 from Merck Millipore. A2780 and IGROV-1 were cultured in RPMI 1640 medium (Gibco, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin (Gibco, Waltham, MA, USA). OVCAR-8 and SK-OV-3 were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Waltham, MA, USA) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were incubated at 37 °C in a 5% CO₂ atmosphere. The presence of mycoplasma was routinely checked with the MycoAlert kit (Lonza, Basel, Switzerland) and only mycoplasma-free cells were used in the experiments.

Ovejero-Sánchez M., Asensio-Juárez G., González M., Puebla P., Vicente-Manzanares M., Pélaez R., González-Sarmiento R, & Herrero A.B. (2022). Panobinostat Synergistically Enhances the Cytotoxicity of Microtubule Destabilizing Drugs in Ovarian Cancer Cells. International Journal of Molecular Sciences, 23(21), 13019.

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Ovarian Cancer Cell Line Culture Protocol

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Ovarian cancer cell lines (COV-413B, IGROV-1, and SK-OV-3) were purchased from Sigma-Aldrich (St. Louis, MO, USA) OVCAR-3 was purchased from ATCC and ADR-RES was purchased from EZBiosystem and grown as recommended in RPMI-1640 or Dulbecco’s Modified Eagle Medium containing 10% FBS at 37 °C in 5% CO₂. The DOT1L inhibitor Pinometostat (EPZ-5676), EPZ004777 and SGC0946 were purchased from Medchemexpress (MCE). Additional information is provided in Supplementary Table 8.

Chava S., Bugide S., Edwards Y.J, & Gupta R. (2021). Disruptor of telomeric silencing 1-like promotes ovarian cancer tumor growth by stimulating pro-tumorigenic metabolic pathways and blocking apoptosis. Oncogenesis, 10(7), 48.

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Culturing Ovarian and Neuroblastoma Cell Lines

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SKOV-3
(ATCC, HTB77), OVCAR-3 (ATCC, HTB-161),
OVCAR-8 (inherited from Laurent Brad’s previous laboratory),
and CAOV-3 (ATCC, HTB75) ovarian cancer cells were grown in complete
DMEM media (Gibco, 11965). IGROV-1 (Sigma, SCC203) and 2008 (kindly
provided by Dr. François X. Claret, University of Texas M.D.
Anderson Cancer Center) ovarian cancer cells were grown in complete
RPMI medium (Gibco, 22400). ES2 (ATCC, CRL-1978) was grown in McCoy’s
5A complete medium (ATCC, 30-2007). BE(2)C (ATCC, CRL-2268), SH-EP1
(ATCC, CRL-2269), SH-SY5Y (ATCC, CRL-2266), KELLY (Sigma, 92110411),
SK-N-AS (Sigma, 94092302), and LAN-5 (COG, http://www.cogcell.org) neuroblastoma
cell-lines were maintained in RPMI1640 media (Gibco, 11875) supplemented
with 10% heat-inactivated FBS. TH-MYCN+/+ cells were derived by mechanical
dissociation of tumors obtained from TH-MYCN homozygous mice^{51 (link)−53 (link)} and were maintained in RPMI1640 media (Gibco, 11875) supplemented
with 20% heat-inactivated FBS, 10–5 mM 2-mercaptoethanol, 1
mM sodium pyruvate, and 1× nonessential amino acids (Gibco, 11140076).

Khazan N., Kim K.K., Hansen J.N., Singh N.A., Moore T., Snyder C.W., Pandita R., Strawderman M., Fujihara M., Takamura Y., Jian Y., Battaglia N., Yano N., Teramoto Y., Arnold L.A., Hopson R., Kishor K., Nayak S., Ojha D., Sharon A., Ashton J.M., Wang J., Milano M.T., Miyamoto H., Linehan D.C., Gerber S.A., Kawar N., Singh A.P., Tabdanov E.D., Dokholyan N.V., Kakuta H., Jurutka P.W., Schor N.F., Rowswell-Turner R.B., Singh R.K, & Moore R.G. (2022). Identification of a Vitamin-D Receptor Antagonist, MeTC7, which Inhibits the Growth of Xenograft and Transgenic Tumors In Vivo. Journal of Medicinal Chemistry, 65(8), 6039-6055.

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Comparative Study of OC Cell Lines

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The present study was performed on 3 OC cell lines, namely the Igrov-1 (Institut Gustave Roussy), SKOV-3 and Ovcar-3 (both American Type Culture Collection) cell lines. SKOV-3 and Igrov-1 cells were cultured in 4.5 g/l DMEM supplemented with 10% FBS and 1% penicillin-streptomycin (PS; Sigma-Aldrich; Merck KGaA) according to the manufacturer's protocol, whereas Ovcar-3 cells was cultured in F12 medium supplemented with 20% FBS and 1% PS (Sigma-Aldrich; Merck KGaA). All cells were incubated in a humidified incubator at 37°C with 5% CO₂. CDDP (Sigma-Aldrich; Merck KGaA) was freshly dissolved in 0.9% NaCl solution, whereas PTX (Sigma-Aldrich; Merck KGaA) was prepared in DMSO and stored at −20°C.

Antoun S., Atallah D., Tahtouh R., Assaf M.D., Moubarak M., Ayoub E.N., Chahine G, & Hilal G. (2019). Glucose restriction combined with chemotherapy decreases telomere length and cancer antigen-125 secretion in ovarian carcinoma. Oncology Letters, 19(2), 1338-1350.

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Diverse Ovarian Cancer Cell Lines

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HEK293T, OV90, HCT116, HT-29, ZR75-30, HCC1954 and BT549 were purchased from American Type Culture Collection (ATCC). OVKATE was purchased from JCRB Cell Bank. OV56, A2780 and IGROV1 were purchased from Sigma. COLO678 was purchased from Leibniz Institute DSMZ. Human ovarian PARP inhibitor and cisplatin-resistant cancer cell lines, PEO1/ABT-888 and OVCAR-1/Cisplatin and their parental cell lines, were gifts from Prof. Scott Kaufmann (Department of Oncology, Mayo Clinic). Cells were maintained under recommended culture conditions. PEO1/ABT-888 and OVCAR-1/Cisplatin were cultured in the media supplemented with 10 μM ABT-888 and 0.5 μg cisplatin, respectively. Cells were regularly tested for mycoplasma contamination.

Zhang C., Guo Q., Chen L., Wu Z., Yan X.J., Zou C., Zhang Q., Tan J., Fang T., Rao Q., Li Y., Shen S., Deng M., Wang L., Gao H., Yu J., Li H., Zhang C., Nowsheen S., Kloeber J., Zhao F., Yin P., Teng C., Lin Z., Song K., Yao S., Yao L., Wu L., Zhang Y., Cheng X., Gao Q., Yuan J., Lou Z, & Zhang J.S. (2023). A ribosomal gene panel predicting a novel synthetic lethality in non-BRCAness tumors. Signal Transduction and Targeted Therapy, 8, 183.

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